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Euphytica

, 214:80 | Cite as

Signatures of divergent selection for cold tolerance in maize

  • Elisabetta Frascaroli
  • Pierangelo Landi
Article
  • 173 Downloads

Abstract

Divergently selected genotypes can be used for detecting the genomic regions affecting the selected trait (selection signature). Moreover, the genetic distances (GDs) among divergently selected lines can be correlated with the agronomic performances of the crosses among them. Using as source the maize F2 of B73 × IABO78, we previously conducted four cycles of divergent recurrent selection and three cycles of divergent selection in inbreeding for cold tolerance at germination. We finally obtained 10 lines selected for low (L) and 10 lines selected for high (H) cold tolerance, which exhibited a notable divergence for both the selected and associated traits. Herein, we investigated the 20 lines and the 28 single diallel crosses among eight random lines (four L and four H); the main objectives were to identify the putative regions controlling the selected and associated traits and to study the relationships between crosses performances and GDs among their parental lines. Allele frequencies at 932 recombination blocks based on 19,220 polymorphic SNPs were obtained for the two lines’ groups; the F ST calculated across sliding windows indicated 18 regions highly divergent between groups. The increasing alleles for cold tolerance were contributed by both parents, consistently with the transgressive segregations previously found. Several regions associated to DG also affected various agronomic traits. The cross performances showed some relationships with the genetic distances among parental lines for traits affected by dominance, provided that all crosses were considered, while these relationships vanished when only L × H crosses were examined.

Keywords

Cold tolerance Divergent selection Genetic distance Selection signature 

Abbreviations

DG

Difference in germination (G9.5–G25)

F1P

Crosses performance

FE

Field emergence

G25

Germination at 25 °C

G9.5

Germination at 9.5 °C

GCA

General combining ability

GD

Genetic distance

GY

Grain yield

H

Lines selected for high cold tolerance

KM

Kernel moisture

L

Lines selected for low cold tolerance

LASSO

Least Absolute Shrinkage and Selection Operator

MP

Mean of parental lines

MPH

Midparent heterosis

MRD

Modified Rogers’ Distance

PFW

Plot fresh weight

PH

Plant height

PS

Pollen shedding date

SCA

Specific combining ability

SFW

Seedling fresh weight

SNP

Single nucleotide polymorphism

UPGMA

Unweighted pair group method arithmetic mean

Notes

Acknowledgements

This study was conducted with the financial support of the University of Bologna, Grant RFO (2009–2014).

Supplementary material

10681_2018_2163_MOESM1_ESM.docx (738 kb)
Supplementary material 1 (DOCX 738 kb)

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© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Agricultural and Food Sciences - DISTALUniversity of BolognaBolognaItaly

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