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Genetic analysis of the fiber quality and yield traits in G. hirsutum background using chromosome segments substitution lines (CSSLs) from Gossypium barbadense

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Abstract

Developing chromosome segments substitution lines (CSSLs) is an effective method for broadening the cotton germplasm resource, and improving the fiber quality and yield traits. In this study, the 1054 F2 individual plants and 116 F2:3 lineages were generated from the two parents of MBI9749 and MBI9915 selected from BC5F3:5 lines which originated from hybridization of CCRI36 and Hai1, and advanced backcrossing and repeated selfing. Genotypes of the parents and F2 population were analyzed. The results showed that 19 segments were introgressed for MBI9749 and 12 segments were introgressed for MBI9915, distributing on 17 linkage groups. The average background recovery rate to the recurrent parent CCRI36 was 96.70% for the two parents. An average of 16.46 segments was introgressed in F2 population. The average recovery rate of 1054 individual plants was 96.85%, and the mean length of sea island introgression segments was 157.18 cM, accounting for 3.15% of detection length. QTL mapping analysis detected 22 QTLs associated with fiber quality and yield traits in the F2 and F2:3 populations. These QTLs distributed on seven chromosomes, and the phenotypic variation was explained ranging from 1.20 to 14.61%. Four stable QTLs were detected in F2 and F2:3 populations, simultaneously. We found that eight QTLs were in agreement with the previous research. Six QTL-clusters were identified for fiber quality and yield traits, in which five QTL-clusters were on chromosome20. The results indicated that most of QTL-clusters always improve the fiber quality and have negative additive effect for yield related traits. This study demonstrated that CSSLs provide basis for fine mapping of the fiber quality and yield traits in future, and could be efficiently used for pyramiding favourable alleles to develop the new germplasms for breeding by molecular marker-assisted selection.

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Acknowledgements

This work was funded by the National Natural Science Foundation of China (31101188), the National Key R&D Program for Crop Breeding (2016YFD0100300), the National Agricultural Science and Technology Innovation Project for CAAS and the Project of Director (161016201733).

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  1. Lixue Guo and Yuzhen Shi have contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, 455000, China

    Lixue Guo, Yuzhen Shi, Juwu Gong, Aiying Liu, Yunna Tan, Wankui Gong, Junwen Li, Tingting Chen, Haihong Shang, Qun Ge, Quanwei Lu & Youlu Yuan

  2. College of Agriculture, The Key Laboratory of Oasis Eco-agriculture of Xingjiang Production and Construction Group, Shihezi University, Shihezi, 832000, China

    Lixue Guo & Jie Sun

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  1. Lixue Guo
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  2. Yuzhen Shi
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  3. Juwu Gong
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  4. Aiying Liu
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  12. Jie Sun
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  13. Youlu Yuan
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Guo, L., Shi, Y., Gong, J. et al. Genetic analysis of the fiber quality and yield traits in G. hirsutum background using chromosome segments substitution lines (CSSLs) from Gossypium barbadense. Euphytica 214, 82 (2018). https://doi.org/10.1007/s10681-018-2158-7

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