Skip to main content

Response to selection for improved nitrogen fixation in common bean (Phaseolus vulgaris L.)

Abstract

Breeding for high symbiotic nitrogen (N) fixation (SNF) in common bean (Phaseolus vulgaris L.) is expected to contribute to reduced application of chemical fertilizers in cropping systems involving common bean. The magnitude of variation and the genetic and phenotypic correlation among seed yield, SNF, estimated as the percentage of nitrogen derived from atmosphere, and related traits were studied in a population of 140 F4-derived F5 recombinant inbred lines, developed from a cross between low- and high-SNF bean genotypes ‘Sanilac’ and ‘Mist’, respectively. The experiment was conducted in a total of five location-years in Ontario, Canada, from 2011 to 2013. These location-years were grouped into stress- and optimum moisture test sites, based on the total precipitation during the growing season. In each test site two nitrogen supply management strategies, SNF-dependent and N fertilizer-dependent, were simulated separately in the field by inoculating the seed with a commercial Rhizobium leguminosarum bv. phaseoli and by application of N fertilizers at 100 kg ha−1, respectively. The genetic variation was significant for seed yield, SNF and related traits. The heritability of the traits ranged from 14 to 71% and 4 to 25% in optimum moisture and in stress environments, respectively. No significant correlation between SNF and seed yield indicated that selection for high SNF does not necessarily lead to greater seed yield and that selection for both traits should be performed simultaneously.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2

References

  • Allard RW (1960) Principles of plant breeding. Wiley, New York

    Google Scholar 

  • Antipchuk AF, Kantselyaruk RM, Rangelova VN, Skochinskaya NN, Tantsyurenko EV (1990) Relation between the photo-assimilation activity indices of leguminous plants and their symbiotic nitrogen fixation. http://eurekamag.com/research/007/737/007737288.php#close. Accessed 13 Nov 2014

  • Appiah FK, Tufuor JK, Amoako-Andoh F (2015) Nitrogen fixation and yield potential of some early-maturing cowpea (Vigna unguiculata (L.) Walp.) lines. J Biol Agr Health Care 5:2224–3208

    Google Scholar 

  • Bänziger M, Betrán FJ, Lafitte HR (1997) Efficiency of high nitrogen selection environment for improving maize for low-nitrogen target environments. Crop Sci 37:1103–1109

    Article  Google Scholar 

  • Bethlenfalvay GJ, Abu Shakra SS, Fisbeck K, Phillips DA (1978) The effect of source-sink manipulations on nitrogen fixation in peas. Physiol Plant 43:31–34

    CAS  Article  Google Scholar 

  • Beversdorf WD (1984) OAC RICO field bean. Can J Plant Sci 64:753–755

    Article  Google Scholar 

  • Bliss FA (1993) Breeding common bean for improved biological nitrogen fixation. Plant Soil 152:71–79

    Article  Google Scholar 

  • Ceccarelli S (1987) Yield potential and drought tolerance of segregating populations of barley in contrasting environments. Euphytica 36:265–273

    Article  Google Scholar 

  • Ceccarelli S (1996) Adaptation to low/high input cultivation. Euphytica 92:203–214

    Article  Google Scholar 

  • Ceccarelli S, Grando S, Impiglia A (1998) Choice of selection strategy in breeding barley for stress environments. Euphytica 103:307–318

    Article  Google Scholar 

  • Díaz-Batalla L, Widholm JM, Fahey-JR GC, Castaňo-Astano-Tostado Paredes-Loäpez EO (2006) Chemical components with health implications in wild and cultivated Mexican common bean seeds (Phaseolus vulgaris L). J Agric Food Chem 54:2045–2052

    Article  PubMed  Google Scholar 

  • Dinh HT, Kaewpradit W, Jogoly S, Vorasoot N, Patanothai A (2013) Biological nitrogen fixation of peanut genotypes with different levels of drought tolerance under mid-season. SABRAO J Breed Genet 45:491–503

    Google Scholar 

  • Eid MH (2009) Estimation of heritability and genetic advance of yield traits in wheat (Triticum aestivum L.) under drought condition. Int J Genet Mol Biol 1:115–120

    Google Scholar 

  • Eskandari M (2012) Identification and localization of quantitative trait loci (QTL) and genes associated with oil concentration in soybean [Glycine max (L.) Merrill.] seed. Dissertation, University of Guelph

  • Fageria NK, Baligar VC, Jones CA (2011) Growth and mineral nutrition of field crops. CRC Press, Boca Raton

    Google Scholar 

  • Fageria NK, Melo LC, Ferreira EPB, Oliveira JP, Knupp AM (2014) Dry matter, grain yield, and yield components of dry bean as influenced by nitrogen fertilization and rhizobia. Commun Soil Sci Plan 45:111–125

    CAS  Article  Google Scholar 

  • Farid M, Navabi A (2015) N2 fixation ability of different dry bean genotypes. Can J Plant Sci 95:1243–1257

    CAS  Article  Google Scholar 

  • Farid M, Earl HJ, Navabi A (2016) Yield stability of dry bean genotypes across nitrogen fixation-dependent and fertilizer-dependent management systems. Crop Sci 56:173–182

    CAS  Article  Google Scholar 

  • Farquhar GD, Ehleringer JR, Hubick KT (1989) Carbon isotope discrimination and photosynthesis. Annu Rev Plant Physiol Plant Mol Biol 40:503–537

    CAS  Article  Google Scholar 

  • Global Biodiversity Information Facilities (2015) Free and open access to biodiversity data. http://www.gbif.org. Accessed 15 June 2015

  • Graham PH (1981) Some problems of nodulation and symbiotic fixation in Phaseolus vulgaris L. A review. Field Crops Res 4:93–112

    Article  Google Scholar 

  • Güler S, Özçelik H (2007) Relationships between leaf chlorophyll and related yield characters in dry bean (Phaseolus vulgaris L.). Asian J Plant Sci 6:700–703

    Article  Google Scholar 

  • Hafeez FY, Ahmad T, Hameed S, Danso SKA, Malik KA (1998) Comparison of direct and indirect methods of measuring nitrogen fixation in field green chickpea genotypes. Pak J Bot 30:199–207

    Google Scholar 

  • Hefni M, Veronica O, Mohamed T, Cornelia W (2010) Folate content in foods commonly consumed in Egypt. J Food Chem 121:540–545

    CAS  Article  Google Scholar 

  • Holland JB (2006) Estimating genetic correlations and their standard errors using multivariate restricted maximum likelihood estimation with SAS Proc MIXED. Crop Sci 46:642–654

    Article  Google Scholar 

  • Holland JB, Nyquist WE, Cervants-Martínez T (2003) Estimating and interpreting heritability for plant breeding. an update Plant Breeding. Rev 22:9–112

    Google Scholar 

  • Jinks JL, Connolly V (1973) Selection for specific and general response to environmental differences. Heredity 30:33–40

    Article  Google Scholar 

  • Johnson SS, Geadelmann JL (1989) Influence of water stress on grain yield response to recurrent selection in maize. Crop Sci 29:558–565

    Article  Google Scholar 

  • Kamfwa K, Cichy KA, Kelly JD (2015) Genome-wide association analysis of symbiotic nitrogen fixation in common bean. Theor Appl Genet 128:1999–2017

    CAS  Article  PubMed  Google Scholar 

  • Kibite S, Evans LE (1984) Causes of negative correlations between grain yield and grain protein concentration in common wheat. Euphytica 33:801–810

    Article  Google Scholar 

  • Knight JD, Verhees F, Van Kessel C, Slinkard AE (1993) Does carbon isotope discrimination correlate with biological nitrogen fixation? Plant Soil 153:151–153

    CAS  Article  Google Scholar 

  • Korkovelos AE, Goulas CK (2011) Divergent mass selection for leaf chlorophyll content measured using chlorophyll meter readings in a maize composite population. Crop Sci 51:1437–1443

    Article  Google Scholar 

  • Kumar Rao JVDK, Dart PJ (1987) Nodulation, nitrogen fixation and nitrogen uptake in pigeonpea [Cajanus cajan (L.) Millsp.] of different maturity groups. Plant Soil 99:255–266

    Article  Google Scholar 

  • Kumarasinghe KS, Kirda C, Mohamed ARAG, Zapata F, Danso SK (1992) Carbon 13 isotope discrimination correlates with biological nitrogen fixation in soybean [Glycine max (L.) Merrill.]. Plant Soil 139:145–147

    CAS  Article  Google Scholar 

  • Liang XQ, Li L, Chen YX, Li H, Liu J, He MM, Ye YS, Tian GM, Lundy M (2013) Dissolved phosphorus losses by lateral seepage from swine manure amendments for organic rice production. Soil Sci Soc Am J 77:765–773

    CAS  Google Scholar 

  • Luthra YP, Seoran IS, Singh R (1983) Ontogenetic interactions between photosynthesis and symbiotic nitrogen fixation in pigeon pea. Ann Appl Biol 103:549–556

    CAS  Article  Google Scholar 

  • Martínez- Roméro E (2003) Diversity of Rhizobium-Phaseolus vulgaris symbiosis: overview and perspectives. Plant Soil 252:11–23

    Article  Google Scholar 

  • Miranda BD, Bliss FA (1991) Selection for increased seed nitrogen accumulation in common bean: implications for improving di-nitrogen fixation and seed yield. Plant Breed 106:301–311

    CAS  Article  Google Scholar 

  • Mural V, Chikkalingaiah R, Hittalmani S (2012) Correlation study for protein content, grain yield and yield contributing traits in quality protein maize (QPM) (Zea mays L.). Electron J Plant Breed 3:649–651

    Google Scholar 

  • Mutlu N, Miklas P, Reiser Coyne JD (2005) Backcross breeding for improved resistance to common bacterial blight in pinto bean (Phaseolus vulgaris L). Plant Breed 124:282–287

    Article  Google Scholar 

  • Nodari RO, Tsai SM, Guzman P, Gilbertson RL, Gepts P (1993) Toward an integrated linkage map of common bean III Mapping genetic factors controlling host-bacteria interactions. Genetics 134:341–350

    CAS  PubMed  PubMed Central  Google Scholar 

  • Park SJ, Buttery BR (1988) Nodulation mutants of white bean (Phaseolus vulgaris L.) induced by ethyl-methane sulphonate. Can J Plant Sci 68:199–202

    CAS  Article  Google Scholar 

  • Peoples MB, Boddy RM, Herridge DF (2002) Quantification of nitrogen fixation. In: Leigh GJ (ed) Nitrogen fixation at the millennium. Elsevier Science, Amsterdam, pp 357–389

    Chapter  Google Scholar 

  • Ramaekers L, Galeano CH, GarzÓn N, Vanderleyden J, Blair MW (2013) Identifying quantitative trait loci for symbiotic nitrogen fixation capacity and related traits in common bean. Mol Breed 31:163–180

    CAS  Article  Google Scholar 

  • Raun WR, Johnson GV (1999) Improving nitrogen use efficiency for cereals production. Agron J 91:357–363

    Article  Google Scholar 

  • Rennie RJ, Kemp GA (1983) N2-fixation in field beans quantified by 15N isotope dilution. II. Effect of cultivars of beans. Agron J 75:645–649

    CAS  Article  Google Scholar 

  • Ribeiro ND, Maziero SM, Prigol M, Nogueira CW, Rosa DP, Possobom MTDF (2012) Mineral concentrations in the embryo and seed coat of common bean cultivars. J Food Compos Anal 26:89–95

    CAS  Article  Google Scholar 

  • Robinson HF, Comstocakn RE, Harvey PH (1949) Estimates of heritability and the degree of dominance in corn. Agron J 41:353–359

    Article  Google Scholar 

  • Scott ME, Michaels TE (1992) Xanthomonas resistance of Phaseolus interspecific cross selections confirmed by field performance. HortScience 27:348–350

    Google Scholar 

  • Shearer G, Kohl DH (1986) N2-fixation in field settings: estimations based on natural δ15N abundance. Aust J Plant Physiol 13:699–756

    CAS  Google Scholar 

  • Souza AA, Boscariol RL, Moon DH, Camargo LEA, Tsai SM (2000) Effects of Phaseolus vulgaris QTL in controlling host-bacteria interactions under two levels of nitrogen fertilization. Gen Mol Biol 23:155–161

    CAS  Article  Google Scholar 

  • Sutton MA, Reis S, Riddick SN, Dragosits U, Nemitz E, Theobald MR, Tang YS, Braban CF, Vieno M, Dore AJ, Mitchell RF, Wanless S, Daunt F, Fowler D, Blackall TD, Milford C, Flechard CR, Loubet B, Massad RS, Cellier P, Personne E, Coheur PF, Clarisse L, Van Damme M, Ngadi Y, Clerbaux C, Skjøth CA, Geels C, Hertel O, Wichink Kruit RJ, Pinder RW, Bash JO, Walker JT, Simpson D, Horvath L, Misselbrook TH, Bleeker A, Dentener F, De Vries W (2013) Towards a climate dependent paradigm of ammonia emission and deposition. Philos Trans R Soc Lond B. doi:10.1098/rstb.2013.0166

    Google Scholar 

  • Thomas CV, Waines JG (1984) Fertile backcross and allotetraploid plants from crosses between tepary beans and common beans. Heredity 75:93–98

    Article  Google Scholar 

  • Van Ginkel M (1994) Bread wheat breeding for yield under drought conditions. In: Rajaram S, Hettel GP (eds) Wheat breeding at CIMMYT: Commemorating 50 years of research in Mexico for Global Wheat Improvement International Maize and Wheat Improvement Center. CIMMYT, Ciudad Obregon, pp 22–29

    Google Scholar 

  • Van Kessel C, Hartley C (2000) Agricultural management of grain legumes: has it led to an increase in nitrogen fixation? Field Crops Res 65:165–181

    Article  Google Scholar 

  • Whitehead WF, Allen FL (1990) High- vs. low-stress yield test environments for selecting superior soybean lines. Crop Sci 30:912–918

    Article  Google Scholar 

  • Yan W, Rajcan I (2002) Biplot analysis of test sites and trait relations of soybean in Ontario. Crop Sci 42:11–20

    Article  PubMed  Google Scholar 

  • Yu K, Park SJ, Poysa V (2000) Marker-assisted selection of common beans for resistance to common bacterial blight: efficacy and economics. Plant Breed 119:411–415

    CAS  Article  Google Scholar 

Download references

Acknowledgements

Technical assistance of Tom Smith, Terry Rupert, BaiLing Zhang, Kathleen Keenan, Josh Good, Melanie Wolters, Anastasia Chechulina, Alison Core, Melinda Drummond, Alison Core, Kristina Dydensborg and financial support of the project by the Ontario Bean Growers, Agriculture and Agri-Food Canada, Agriculture Adaptation Council, and the Ontario Ministry of Research and Innovation are duly acknowledged.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Alireza Navabi.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Farid, M., Earl, H.J., Pauls, K.P. et al. Response to selection for improved nitrogen fixation in common bean (Phaseolus vulgaris L.). Euphytica 213, 99 (2017). https://doi.org/10.1007/s10681-017-1885-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s10681-017-1885-5

Keywords

  • Common bean
  • Symbiotic nitrogen fixation
  • Genetic gain
  • Heritability
  • Response to selection