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Euphytica

, 213:84 | Cite as

Genotyping-by-sequencing of waxy and glossy near-isogenic broccoli lines

  • Sandra E. Branham
  • Mark W. Farnham
Article
  • 253 Downloads

Abstract

Wild-type Brassica oleracea L. have matte blue-green leaves caused by an interaction between leaf pigmentation and a waxy bloom coating the surface. Glossy mutants have reduced and/or altered epicuticular wax giving the leaves a shiny green appearance and have been identified in most B. oleracea crop varieties, including cauliflower, kale, broccoli, Brussels sprouts, cabbage, and collard. The genetic basis of glossy mutants has not been studied in B. oleracea. Glossiness can confer resistance to multiple herbivores mediated by modification of herbivore feeding behavior and foraging efficiency of their predators. The USDA-ARS-U.S. Vegetable Laboratory released two pairs of near-isogenic broccoli lines (NILs) that visibly differ only for glossiness, providing ideal germplasm for the genetic study of epicuticular wax in B. oleracea. Genotyping-by-sequencing resulted in hundreds of polymorphisms between each pair of NILs. Polymorphisms were identified in or near three different wax synthesis genes suggesting the two glossy mutants were caused by alteration of different steps of the pathway.

Keywords

Glossy Wax Brassica oleracea Broccoli GBS NIL 

Notes

Acknowledgements

This study was funded by the United States Department of Agriculture, CRIS Project No. 6080-21000-018-00D.

Supplementary material

10681_2017_1873_MOESM1_ESM.xlsx (48 kb)
Supplementary material 1 (XLSX 47 kb)
10681_2017_1873_MOESM2_ESM.xlsx (44 kb)
Supplementary material 2 (XLSX 44 kb)

References

  1. Anstey TH, Moore JF (1954) Inheritance of glossy foliage and cream petals in Green Sprouting broccoli. J Heredity 45:39–41CrossRefGoogle Scholar
  2. Bradbury PJ, Zhang Z, Kroon DE et al (2007) TASSEL: software for association mapping of complex traits in diverse samples. Bioinformatics 23:2633–2635CrossRefPubMedGoogle Scholar
  3. Chatterton N, Hannah W, Powell J, Lee D (1975) Photosynthesis and transpiration of bloom and bloomless sorghum. Can J Plant Sci 55(2):641–643CrossRefGoogle Scholar
  4. Eigenbrode SD, Kabalo NN (1999) Effects of Brassica oleracea waxblooms on predation and attachment by Hippodamia convergens. Entomol Exp Appl 91:125–130CrossRefGoogle Scholar
  5. Eigenbrode SD, Stoner KA, Shelton AM, Kain WC (1991) Characteristics of leaf waxes of Brassica oleracea associated with resistance to diamondback moth. J Econ Entomol 83:1609–1618CrossRefGoogle Scholar
  6. Elshire RJ, Glaubitz JC, Sun Q et al (2011) A robust, simple genotyping-by-sequencing (GBS) approach for high diversity species. PLoS ONE 6:e19379CrossRefPubMedPubMedCentralGoogle Scholar
  7. Farnham M (2010) Glossy and nonglossy near-isogenic lines USVL115-GL, USVL115-NG, USVL188-GL, and USVL188-NG of broccoli. HortScience 45:660–662Google Scholar
  8. Farnham MW, Elsey KD (1995) Recognition of Brassica oleracea L. resistance against the silverleaf whitefly. HortScience 30:343–347Google Scholar
  9. Gentry GL, Barbosa P (2006) Effects of leaf epicuticular wax on the movement, foraging behavior, and attack efficacy of Diaeretiella rapae. Entomol Exp Appl 121:115–122CrossRefGoogle Scholar
  10. Glaubitz JC, Casstevens TM, Lu F et al (2014) TASSEL-GBS: a high capacity genotyping by sequencing analysis pipeline. PLoS ONE 9:e90346CrossRefPubMedPubMedCentralGoogle Scholar
  11. Hall DM, Matus AI, Lamberton J, Barber HN (1965) Infra-specific variation in wax on leaf surfaces. Aust J Biol Sci 18:323–332CrossRefGoogle Scholar
  12. Hariprasad KV, van Emden HF (2010) Mechanisms of partial plant resistance to diamondback moth (Plutella xylostella) in Brassicas. Int J Pest Manag 56:15–22CrossRefGoogle Scholar
  13. Jenks MA, Joly RJ, Peters PJ et al (1994) Chemically induced cuticle mutation affecting epidermal conductance to water vapor and disease susceptibility in Sorghum bicolor (L.) Moench. Plant Physiol 105:1239–1245CrossRefPubMedPubMedCentralGoogle Scholar
  14. Jenks MA, Tuttle HA, Eigenbrode SD, Feldmann KA (1995) Leaf epicuticular waxes of the eceriferum mutants in Arabidopsis. Plant Physiol 108:369–377CrossRefPubMedPubMedCentralGoogle Scholar
  15. Jenks MA, Rashotte AM, Tuttle HA, Feldmann KA (1996) Mutants in Arabidopsis thaliana altered in epicuticular wax and leaf morphology. Plant Physiol 110:377–385CrossRefPubMedPubMedCentralGoogle Scholar
  16. Jordan WR, Shouse PJ, Blum A, Miller FR, Monk RL (1984) Environmental physiology of sorghum. II. Epicuticular wax load and cuticular transpiration. Crop Sci 24:1168–1173CrossRefGoogle Scholar
  17. Joubès J, Raffaele S, Bourdenx B et al (2008) The VLCFA elongase gene family in Arabidopsis thaliana: phylogenetic analysis, 3D modelling and expression profiling. Plant Mol Biol 67:547–566CrossRefPubMedGoogle Scholar
  18. Li H, Durbin R (2009) Fast and accurate short read alignment with Burrows-Wheeler Transform. Bioinformatics 25:1754–1760CrossRefPubMedPubMedCentralGoogle Scholar
  19. Li H, Durbin R (2010) Fast and accurate long-read alignment with Burrows-Wheeler Transform. Bioinformatics 26(5):589–595CrossRefPubMedPubMedCentralGoogle Scholar
  20. Li F, Wu X, Lam P et al (2008) Identification of the wax ester synthase/acyl-coenzyme A: diacylglycerol acyltransferase WSD1 required for stem wax ester biosynthesis in Arabidopsis. Plant Physiol 148:97–107CrossRefPubMedPubMedCentralGoogle Scholar
  21. Macey MJK (1974) Wax synthesis in Brassica oleracea as modified by trichloroacetic acid and glossy mutations. Phytochemistry 13:1353–1358CrossRefGoogle Scholar
  22. Macey MJK, Barber HN (1970) Chemical genetics of wax formation on leaves of Brassica oleracea. Phytochemistry 9:13–23CrossRefGoogle Scholar
  23. McNevin JP, Woodward W, Hannoufa A, Feldmann KA, Lemieux B (1993) Isolation and characterization of eceriferum (cer) mutants induced by T-DNA insertions in Arabidopsis thaliana. Genome 36:610–618CrossRefPubMedGoogle Scholar
  24. Millar AA, Kunst L (1997) Very-long-chain fatty acid biosynthesis is controlled through the expression and specificity of the condensing enzyme. Plant J 12:121–131CrossRefPubMedGoogle Scholar
  25. North C, Priestley WG (1962) A glossy-leaved mutant of Brussels Sprout. HortResearch 1:95–99Google Scholar
  26. Parkin IAP, Koh C, Tang H et al (2014) Transcriptome and methylome profiling reveals relics of genome dominance in the mesopolyploid Brassica oleracea. Genome Biol 15:R77CrossRefPubMedPubMedCentralGoogle Scholar
  27. Rowland O, Zheng H, Hepworth SR et al (2006) CER4 encodes an alcohol-forming fatty acyl-coenzyme A reductase involved in cuticular wax production in Arabidopsis. Plant Physiol 142:866–877CrossRefPubMedPubMedCentralGoogle Scholar
  28. Stoner KA (1990) Glossy leaf wax and plant resistance to insects in Brassica oleracea under natural infestation. Environ Entomol 19:730–739CrossRefGoogle Scholar
  29. Stoner KA (1992) Density of imported cabbageworms (Lepidoptera: Pieridae), cabbage aphids (Homoptera: Aphididae), and flea beetles (Coleoptera: Chrysomelidae) on glossy and trichome-bearing lines of Brassica oleracea. J Econ Entomol 85:1023–1030CrossRefGoogle Scholar
  30. Swarts K, Li H, Romero Navarro JA, et al (2014) Novel methods to optimize genotypic imputation for low-coverage, next-generation sequence data in crop plants. The Plant Genome 7:0Google Scholar
  31. Thompson KF (1963) Resistance to the cabbage aphid (Brevicoryne brassicae) in Brassica plants. Nature 198:209CrossRefGoogle Scholar
  32. Ulmer B, Gillott C, Woods D, Erlandson M (2002) Diamondback moth, Plutella xylostella (L.), feeding and oviposition preferences on glossy and waxy Brassica rapa (L.) lines. Crop Prot 21:327–331CrossRefGoogle Scholar
  33. Wang M, Farnham M, Nannes J (1999) Ploidy of broccoli regenerated from microspore culture versus anther culture. Plant Breed 118:249–252CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht (outside the USA) 2017

Authors and Affiliations

  1. 1.USDA-ARSU.S. Vegetable LaboratoryCharlestonUSA

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