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Euphytica

, Volume 211, Issue 1, pp 91–101 | Cite as

Validation and diagnostic marker development for a genetic region associated with wheat yellow mosaic virus resistance

  • Jin Xiao
  • Xueluan Chen
  • Zhitian Xu
  • Jiao Guo
  • Zhenzhen Wu
  • Haiyan Wang
  • Xiaobiao Zhu
  • Mingjuan Nie
  • Tongde Bie
  • Shunhe Cheng
  • TingTing Zhu
  • Mingcheng Luo
  • Frank M. You
  • Xiue Wang
Article
  • 366 Downloads

Abstract

Wheat yellow mosaic disease (WYM) became more serious in China in the recent years. Cultivation of resistant varieties is the major strategy for disease control. To identify WYM resistant genes in Chinese wheat cultivar ‘Yining Xiaomai’, a population of 106 recombinant inbred lines (RIL) from a cross between Yining Xiaomai and Zhen 9523 (WYM-susceptible) was generated. Phenotypic data of WYM resistance of the RILs were collected over three successive years in a naturally infected field nursery. A linkage map consisting of 29 genetic linkage groups involving 144 SSR or EST markers was constructed, using the JoinMap 4.0 software. By composite interval mapping using IciMapping 4.0, a stable major QTL associated with WYM resistance, designated QYm.nau-2D, was mapped to chromosome 2D which explained up to 93.2 % of the phenotypic variance. More linkage markers including 11 SNP and 11 STS markers were developed to saturate the QYm.nau-2D region. The presence of WYM resistance was further validated using a resistant RIL derived F2 population consisting 318 lines. A high resolution map at QYm.nau-2D region was re-constructed which established a basis for map-based cloning of the resistant gene. Four markers were validated to be diagnostic markers to discriminate 195 breeding lines with known pedigree. These markers could be used in marker-assisted selection to breeding WYM resistance.

Keywords

Disease resistance Linkage map Molecular marker Quantitative trait locus Wheat yellow mosaic 

Notes

Acknowledgments

The research was supported by National Natural Science Foundation of China (Grant No. 31171541), the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), the Technology Support Program of Jiangsu Province (Grant No. BE2015352), the Program of Introducing Talents of Discipline to Universities (Grant No. B08025), Project Development of Seed Industry for Shanghai Agricultural World (2012), Fundamental Research Funds for the Central Universities (Grant No. KYZ201202), high-level talents in six industries of Jiangsu Province and the Project No. 7 from Science and High-Tech Based Major Program of Agriculture Committee of Shanghai Municipal Administration (Grant No. 20127). We are grateful to Dr. Robert McIntosh for his useful comments about the research and the critical reading and revise of the manuscript.

Authors’ contributions

XJ, WXE, WHY and CXH designed experimental plan. GJ, CXL, WZZ and XZT performed experiments. NMJ, BTD, ZXB evaluated WYM resistance. ZTT, LMC and FY designed the SNP markers. XJ, WXE, GJ and XZT wrote the manuscript. All authors have read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10681_2016_1731_MOESM1_ESM.pdf (954 kb)
Supplementary Fig. 1: RT-PCR detection of WSSMV by primer pairs WMVCPF/WMVCPR (a) and Wp1(+)/Wp2(−) (b) and of WSSMV by (W2(+)/W9(−) (c) and WSSMVF/Oligo-d(T)Not1) (d), using the tubulin gene (e) for normalization of the cDNA concentration Supplementary Fig. 2: Linkage maps for the Yining Xiaomai × Zhen 9523 F2:8 RIL population. Numbers on the left are genetic distances in centiMorgans (cM). Names of the molecular markers are shown on the right Supplementary Fig. 3: Chromosome bin assignment of marker Xcfd267 using Chinese Spring nulli-tetrasomic and deletion lines (PDF 953 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Jin Xiao
    • 1
  • Xueluan Chen
    • 1
  • Zhitian Xu
    • 1
  • Jiao Guo
    • 1
  • Zhenzhen Wu
    • 1
  • Haiyan Wang
    • 1
  • Xiaobiao Zhu
    • 1
  • Mingjuan Nie
    • 1
  • Tongde Bie
    • 2
  • Shunhe Cheng
    • 2
  • TingTing Zhu
    • 3
  • Mingcheng Luo
    • 3
  • Frank M. You
    • 4
  • Xiue Wang
    • 1
  1. 1.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics InstituteNanjing Agricultural University/JCIC-MCPNanjingChina
  2. 2.Department of Wheat BreedingAgricultural Sciences Institute of the Lixiahe DistrictYangzhouChina
  3. 3.Department of Plant SciencesUniversity of CaliforniaDavisUSA
  4. 4.Agriculture and Agri-Food CanadaMordenCanada

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