, Volume 207, Issue 1, pp 213–224 | Cite as

Root traits contributing to drought tolerance of synthetic hexaploid wheat in a greenhouse study

  • Steven R. Becker
  • Patrick F. Byrne
  • Scott D. Reid
  • William L. Bauerle
  • John K. McKay
  • Scott D. Haley


Drought stress imposes major limits on wheat (Triticum aestivum L.) yield and is predicted to increase in frequency due to climate change. The aim of this study was to explore the potential of synthetic hexaploid wheat (SHW) to improve productivity of winter wheat under drought stress. Six SHW lines and four winter wheat cultivars from the U.S. Great Plains were evaluated in 1 m × 10 cm plastic tubes under drought-stressed and well-watered conditions in a greenhouse study. Root morphology, biomass, stomatal attributes, plant water relations, and the response of these traits to drought stress were measured. Traits significantly (P < 0.05) correlated with a drought tolerance index included root biomass in the bottom third of the tubes, length of the longest root, stomatal conductance, and production of small diameter roots. Plasticity for root biomass allocation to greater depths showed a strong association with maintenance of plant water status. Synthetic line SYN-201 ranked highest for deep root biomass and length of the longest root under stress, and demonstrated plasticity by shifting root biomass production from the upper third to the bottom third of the tubes when stressed. Digital analysis of root morphology indicated that SYN-201, SYN-290, and cultivar Byrd produced large amounts of small diameter roots at depth. SYN-396 showed high stomatal density and reduced stomatal aperture while maintaining leaf growth when stressed despite a lack of deep roots. Trait variation in the SHW lines may contribute beneficial drought tolerance to Great Plains-adapted cultivars through introgression of novel allelic diversity.


Drought tolerance Root morphology Root tubes Trait plasticity Triticum aestivum 



Funding for this research was provided by U.S. Department of Agriculture-National Institute of Food and Agriculture grants 2008-55100-04509 and 2011-68002-30029. We appreciate technical assistance provided by Ms. Jessica McGowan and Ms. Jennifer Matsuura and valuable comments on this manuscript by Dr. Greg McMaster.

Supplementary material

10681_2015_1574_MOESM1_ESM.docx (20 kb)
Supplementary material 1 (DOCX 19 kb)


  1. Baenziger PS, Beecher B, Graybosch RA, Baltensperger DD, Nelson L, Krall JM, Mcvey DV, Watkins JE, Hatchett JH, Chen M (2004) Registration of Goodstreak wheat. Crop Sci 44:1473–1474CrossRefGoogle Scholar
  2. Barrs HD, Weatherley PE (1962) A re-examination of relative turgidity technique for estimating water deficits in leaves. Austr JBiol Sci 15:413–428Google Scholar
  3. Blum A (2009) Effective use of water (EUW) and not water-use efficiency (WUE) is the target of crop yield improvement under drought stress. Field Crops Res 112:119–123CrossRefGoogle Scholar
  4. Blum A (2011) Plant breeding for water-limited environments. Springer Science + Business Media, LLC, New YorkCrossRefGoogle Scholar
  5. Cavanagh CR, Chao S, Wang S, Huang BE, Stephen S, Kiani S, Forrest K, Saintenac C, Brown-Guedira GL, Akhunova A, See D, Bai G, Pumphrey M, Tomar L, Wong D, Kong S, Reynolds M, da Silva ML, Bockelman H, Talbert L, Anderson JA, Dreisigacker S, Baenziger S, Carter A, Korzun V, Morrell PL, Dubcovsky J, Morell MK, Sorrells ME, Hayden MJ, Akhunov E (2013) Genome-wide comparative diversity uncovers multiple targets of selection for improvement in hexaploid wheat landraces and cultivars. Proc Natl Acad Sci USA 110:8057–8062PubMedCentralCrossRefPubMedGoogle Scholar
  6. Chenu K, Cooper M, Hammer GL, Mathews KL, Dreccer MF, Chapman SC (2011) Environment characterization as an aid to wheat improvement: interpreting genotype-environment interactions by modelling water-deficit patterns in North-Eastern Australia. J Exp Bot 62:1743–1755CrossRefPubMedGoogle Scholar
  7. Dai A (2013) Increasing drought under global warming inobservations and models. Nature Climate Change 3:52–58CrossRefGoogle Scholar
  8. Dreisigacker S, Kishii M, Lage J, Warburton M (2008) Use of synthetic hexaploid wheat to increase diversity for CIMMYT bread wheat improvement. Austr JAgric Res 59:413–420CrossRefGoogle Scholar
  9. Ehdaie B, Layne AP, Waines JG (2012) Root system plasticity to drought influences grain yield in bread wheat. Euphytica 186:219–232CrossRefGoogle Scholar
  10. Ekanayake IJ, O’Toole JC, Garrity DP, Masajo TM (1985) Inheritance of root characters and their relations to drought resistance in rice. Crop Sci 25:927–933CrossRefGoogle Scholar
  11. Franks PJ, Drake PL, Beerling DJ (2009) Plasticity in maximum stomatal conductance constrained by negative correlation between stomatal size and density: an analysis using Eucalyptus globulus. Plant Cell Environ 32:1737–1748CrossRefPubMedGoogle Scholar
  12. Gill KS, Lubbers EL, Gill BS, Raupp WJ, Cox TS (1991) A genetic linkage map of Triticum tauschii (DD) and its relationship to the D genome of bread wheat (AABBDD). Genome 34:362–374CrossRefGoogle Scholar
  13. Gregory PJ, Bengough AG, Grinev D, Schmidt S, Thomas WTB, Wojciechowski T, Young IM (2009) Root phenomics of crops: opportunities and challenges. Funct Plant Biol 36:922–929CrossRefGoogle Scholar
  14. Haley SD, Quick JS, Johnson JJ, Peairs FB, Stromberger JA, Clayshulte SR, Clifford BL, Rudolf JB, Seabourn BW, Chung OK, Jin Y, Kolmer JA (2005) Registration of ‘Hatcher’ wheat. Crop Sci 45:2654–2655CrossRefGoogle Scholar
  15. Haley SD, Johnson JJ, Peairs FB, Quick JS, Stromberger JA, Clayshulte SR, Butler JD, Rudolph JB, Seabourn BW, Bai G, Jin Y, Kolmer J (2007) Registration of ‘Ripper’ Wheat. J Plant Reg 1:1–6CrossRefGoogle Scholar
  16. Haley SD, Johnson JJ, Peairs FB, Stromberger JA, Hudson EE, Seifert SA, Kottke RA, Valdez VA, Rudolph JB, Bai G, Chen X, Bowden RL, Jin Y, Kolmer JA, Chen M-S, Seabourn BW (2012) Registration of ‘Byrd’ Wheat. JPlant Reg 6:302–305CrossRefGoogle Scholar
  17. Hetherington AM, Woodward FI (2003) The role of stomata in sensing and driving environmental change. Nature 424:901CrossRefPubMedGoogle Scholar
  18. Hopkins WG, Hüner NPA (2008) Introduction to plant physiology. John Wiley & Sons, HobokenGoogle Scholar
  19. Lopes MS, Reynolds MP (2010) Partitioning of assimilates to deeper roots is associated with cooler canopies and increased yield under drought in wheat. Funct Plant Biol 37:147–156CrossRefGoogle Scholar
  20. Lopes MS, Reynolds MP (2011) Drought adaptive traits and wide adaptation in elite lines derived from resynthesized hexaploid wheat. Crop Sci 51:1617–1626CrossRefGoogle Scholar
  21. Manschadi AM, Christopher J, Devoil P, Hammer GL (2006) The role of root architectural traits in adaptation of wheat to water-limited environments. Funct Plant Biol 33:823–837CrossRefGoogle Scholar
  22. McFadden ES, Sears ER (1944) The artificial synthesis of Triticum spelta. Rec Genet Soc Amer 13:26–27Google Scholar
  23. Mujeeb-Kazi A, Hettel GP (eds) (1995) Utilizing wild grass biodiversity in wheat improvement: 15 years of wide cross research at CIMMYT. CIMMYT Research Report No. 2, MexicoGoogle Scholar
  24. Narayanan S, PrasadPV Vara (2014) Characterization of a spring wheat association mapping panel for root traits. Agron J106:1593–1604CrossRefGoogle Scholar
  25. Narayanan S, Mohan A, Gill KS, Vara Prasad PV (2014) Variability of root traits in spring wheat germplasm. PLoS ONE 9:e100317PubMedCentralCrossRefPubMedGoogle Scholar
  26. Palta JA, Chen X, Milroy SP, Rebetzke GJ, Dreccer MF, Watt M (2011) Large root systems: are they useful in adapting wheat to dry environments? Funct Plant Biol 38:347–354CrossRefGoogle Scholar
  27. Praba ML, Cairns JE, Babu RC, Lafitte HR (2009) Identification of physiological traits underlying cultivar differences in drought tolerance in rice and wheat. J Agron Crop Sci 195:30–46CrossRefGoogle Scholar
  28. Reynolds M, Dreccer F, Trethowan R (2007) Drought-adaptive traits derived from wheat wild relatives and landraces. J Exp Bot 58:177–186CrossRefPubMedGoogle Scholar
  29. Sayar R, Khemira H, Kharrat M (2007) Inheritance of deeper root length and grain yield in half-diallel durum wheat (Triticum durum) crosses. Ann Appl Biol 151:213–220CrossRefGoogle Scholar
  30. Trethowan RM, Mujeeb-Kazi A (2008) Novel germplasm resources for improving environmental stress tolerance of hexaploid wheat. Crop Sci 48:1255–1265CrossRefGoogle Scholar
  31. van Ginkel M, Ogbonnaya F (2007) Novel genetic diversity from synthetic wheats in breeding cultivars for changing production conditions. Field Crops Res 104:86–94CrossRefGoogle Scholar
  32. WHEAT (2014) Wheat: vital grain of civilization and food security 2013 Annual Report, CGIAR Research Program on Wheat, MexicoGoogle Scholar
  33. Ytting NK, Andersen SB, Thorup-Kristensen K (2014) Using tube rhizotrons to measure variation in depth penetration rate among modern North-European winter wheat (Triticum aestivum L.) cultivars. Euphytica 199:233–245CrossRefGoogle Scholar
  34. Zadoks JC, Chang TT, Konzak CF (1974) A decimal code for the growth stages of cereals. Weed Res 14:415–421CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Steven R. Becker
    • 1
    • 2
  • Patrick F. Byrne
    • 1
  • Scott D. Reid
    • 1
  • William L. Bauerle
    • 3
  • John K. McKay
    • 4
  • Scott D. Haley
    • 1
  1. 1.Department of Soil and Crop SciencesColorado State UniversityFort CollinsUSA
  2. 2.Beck’s Superior HybridsAtlantaUSA
  3. 3.Department of Horticulture and Landscape ArchitectureColorado State UniversityFort CollinsUSA
  4. 4.Department of Bioagricultural Sciences and Pest ManagementColorado State UniversityFort CollinsUSA

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