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Euphytica

, Volume 206, Issue 1, pp 149–158 | Cite as

Relationship between CMS-specific mitochondrial structures and pollen abortive phenotype in rice CMS lines

  • G. Q. Zhu
  • X. L. Tan
  • Y. Zhao
  • Q. Y. Zi
  • Y. Z. Zheng
  • C. Q. Yan
  • T. T. He
  • C. H. Sun
  • D. J. Huang
  • Y. L. Tan
  • J. Xu
  • J. C. Wen
Article
  • 267 Downloads

Abstract

Cytoplasmic male sterile (CMS) lines varied at pollen abortive phenotypes (PAP) have been used effectively for production of hybrid seeds in rice. To investigate the relationship between PAP of CMS lines and CMS-specific mitochondrial genes or structures in rice, CMS lines varied at PAP developed on 18 cytoplasmic sources were examined with seven PCR primers specific to three CMS-specific mitochondrial regions. The 18 CMS lines were classified into stained abortive (SA) and typical abortive (TA) types according to their PAP. Two CMS cytoplasms-specific PCR fragments, by which genotypes between the CMS lines and their maintainers were distinguished, were identified with primer LD29 or LD30. Furthermore, it was discovered that phenotypes of SA-CMS and TA-CMS were related to CMS-specific mitochondrial genes or structures, LD-orf310, atp6-orf79-like, and orf288-like structures. The results suggested that CMS phenotypes were mainly controlled by cytoplasmic genes, and it could be speculated that the SA-CMS phenotype was related to LD-orf310 and atp6-orf79-like structure, while TA-CMS phenotype was related to WA352.

Keywords

Cytoplasmic male sterility (CMS) CMS-specific mitochondrial structure Stained pollen abortion Typical pollen abortion Rice 

Notes

Acknowledgments

We thank Dr. X.C. Zhao for critically reviewing the manuscript. This research was financially supported by China National Foundation (Project No. 31060058).

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standard

The authors note that this research is performed and reported in accordance with ethical standards of the scientific conduct. We confirm to have the authority to publish this work and that the manuscript has not been published before and is not under consideration for publication elsewhere.

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • G. Q. Zhu
    • 1
    • 2
  • X. L. Tan
    • 1
    • 2
  • Y. Zhao
    • 1
    • 2
  • Q. Y. Zi
    • 1
    • 2
  • Y. Z. Zheng
    • 1
    • 2
  • C. Q. Yan
    • 1
    • 2
  • T. T. He
    • 1
    • 2
  • C. H. Sun
    • 1
    • 2
  • D. J. Huang
    • 1
    • 2
  • Y. L. Tan
    • 1
    • 2
  • J. Xu
    • 1
    • 2
  • J. C. Wen
    • 1
    • 2
  1. 1.Rice Research Institute, Yunnan Agricultural University (RRI-YAU)KunmingChina
  2. 2.Yunnan Engineering Research Center for Japonica Hybrid RiceKunmingChina

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