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Euphytica

, Volume 205, Issue 3, pp 773–783 | Cite as

Interaction of the mutant genes B, og c , hp and t in the coloring of tomato fruit

  • Thiago Matos Andrade
  • Wilson Roberto Maluf
  • Celso Mattes de Oliveira
  • Luiz Antônio Augusto Gomes
  • Daniela Costa Santos
  • Regis de Castro Carvalho
  • Ranoel José de Sousa Gonçalves
  • Álvaro Carlos Gonçalves Neto
Article

Abstract

The aim of this study was to assess the effect of the mutant genes B, og c , hp, and t on the fruit color of tomato genotypes. Two tomato lines (TOM-498 and TOM-499) with BB genotype composition (high β-carotene homozygous) were used in hybrid combinations with 7 tomato lines with different genotype compositions in loci og c , hp, or t—Floradade, TOM-596, TOM-544, NC-8276, NC-2Y, Florida 7775, and Florida 7781. A randomized complete block design with 24 treatments and four replications was used. Both external and internal fruit color were assessed with a Minolta CR-400 colorimeter in the CIE L*, a* and b* mode. Hue and chroma readings were taken at four different points (epidermis, pericarp, placenta, and columella) of the fruit. For high β-carotene heterozygous hybrids B + /B, hue angle indicated an orange color (since these hybrids did not come to equal the genotypes of normal coloring, red), even when genes promoting lycopene synthesis (og c , hp) were deployed as heterozygous genotypes. Fruit of high β-carotene heterozygous hybrids, without t, og c , or hp, had chroma values similar to fruit of homozygous high β-carotene lines. The use of the og c allele in heterozygosity led to higher chroma values in high β-carotene heterozygous genotypes in relation to the values found in high β-carotene heterozygous genotypes not bearing the og c gene. The og c homozygous lines had hue values that did not differ from those in normal genotypes in the epidermis and pericarp, but showed a significant shift towards red in the placenta and in the columella.

Keywords

Solanum lycopersicum Enriched food Lycopene Beta-carotene Plant breeding 

Notes

Acknowledgments

The authors thank the following Brazilian institutions that provide support for this research Project: FAPEMIG-Fundação de Amparo à Pesquisa do Estado de Minas Gerais, CNPq-Conselho Nacional de Desenvolvimento Científico e Tecnológico, FINEP—Financiadora de Estudos e Projetos/MCT-Ministério de Ciência e Tecnologia/FNDCT—Fundo Nacional de Desenvolvimento Científico e Tecnológico, CAPES-Coordenação de Aperfeiçoamento de Pessoal de Nível Superior/MEC-Ministério da Educação Brasileiro, UFLA-Universidade Federal de Lavras, HortiAgro Sementes S.A., FUNDECC-Fundação para o Desenvolvimento Científico e Tecnológico, and FAEPE-Fundação de Apoio ao Ensino, Pesquisa e Extensão.

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Thiago Matos Andrade
    • 1
  • Wilson Roberto Maluf
    • 2
  • Celso Mattes de Oliveira
    • 2
  • Luiz Antônio Augusto Gomes
    • 2
  • Daniela Costa Santos
    • 2
  • Regis de Castro Carvalho
    • 3
  • Ranoel José de Sousa Gonçalves
    • 4
  • Álvaro Carlos Gonçalves Neto
    • 5
  1. 1.Departamento de Engenharia AgronômicaUniversidade Federal de SergipeSão CristóvãoBrazil
  2. 2.Departamento de AgriculturaUFLALavrasBrazil
  3. 3.Departamento de BiologiaUFLALavrasBrazil
  4. 4.Centro de Desenvolvimento Sustentável do SemiáridoUFCGSuméBrazil
  5. 5.Centro de Ciências Humanas, Sociais e AgráriasUFPBBananeirasBrazil

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