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Mapping and evaluating quantitative trait loci for blast resistance under natural infection conditions using an advanced backcross population in rice

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Abstract

Blast (caused by Magnaporthe oryzae B. Couch) is an economically important and mutable disease of rice (Oryza sativa L.). To analyze the genetic mechanism of blast resistance in rice cultivar CR185; we used CR185 as a donor parent to establish a BC3F1 and derived BC3F2 backcross population in the Zhenshan 97B (ZS97B) background. By challenging the population with natural infection in 2008 and 2009, 11 blast resistance quantitative trait loci (QTLs) were identified. Among them, four major QTLs, qBR-1a, qBR-4d, qBR-6a, and qBR-12a, had large effects during four observation times. QTL effect analyses suggested that resistance increased with the number of QTLs. The major QTL, qBR-6a, which had the largest effect, was fine-mapped to a 100-kb DNA fragment that encompassed the Pi2/Pi9 locus. Sequence comparisons revealed that the Pi allele in CR185 was different from the known alleles of Pi2/Pi9. Our results revealed that accumulation of several QTLs is an effective way to develop highly resistant cultivars.

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Acknowledgments

This work was partially supported by Grants from the National High Technology Research (2012AA101102, 2012AA10A303), the National Program on Research and Development of Transgenic Plants of China (2011ZX08001-002), the Foundation of the Ministry of Agriculture (CARS-01-03, 2011-Z66), and the National Natural Science Foundation of China (31171617) and the Bill & Melinda Gates Foundation.

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    Authors and Affiliations

    1. National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), and National Center of Crop Molecular Breeding, Huazhong Agricultural University, Wuhan, 430070, China

      Haichao Jiang, Bin Yan, Yibo Li, Guanjun Gao, Qinglu Zhang, Jinghua Xiao, Caiguo Xu & Yuqing He

    2. The Enshi Academy of Agricultural Science, Enshi, 445000, Hubei, China

      Taipin Duan

    3. College of Life Science, Heilongjiang University, Harbin, 150080, China

      Gonghao Jiang

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    1. Haichao Jiang
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    Correspondence to Yuqing He.

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    Haichao Jiang and Bin Yan contributed equally to this work.

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    Jiang, H., Yan, B., Duan, T. et al. Mapping and evaluating quantitative trait loci for blast resistance under natural infection conditions using an advanced backcross population in rice. Euphytica 204, 121–133 (2015). https://doi.org/10.1007/s10681-014-1347-2

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