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Euphytica

, Volume 202, Issue 2, pp 307–316 | Cite as

Genotype-specific changes associated to early synthesis of autotetraploids in wild potato species

  • Riccardo Aversano
  • Maria-Teresa Scarano
  • Giovanna Aronne
  • Immacolata Caruso
  • Vincenzo D’Amelia
  • Veronica De Micco
  • Carlo Fasano
  • Pasquale Termolino
  • Domenico Carputo
Article

Abstract

Polyploidy is an important factor in plant evolution that may trigger drastic genome reorganization and phenotypic differentiation. In the last decade, extensive studies have been carried to understand the consequences of allopolyploidization, where the effects of ploidy change may be confounded by interspecific hybridization. By contrast, less is known on autopolyploidization, which only involves doubling of homologous chromosomes. This study was undertaken to assess leaf anatomical modifications and gene expression changes occurring after doubling the somatic chromosome complement of diploid (2n = 2x = 24) potato species Solanum commersonii Dunal and S. bulbocastanum Dunal. Polyploidization did not induce qualitative changes in leaf structure and, for several leaf traits, anatomic modifications were stochastic. In addition, in both species a diploid superiority was generally observed, suggesting the occurrence of a high-ploidy syndrome. Expression change study was carried out on eight important cell cycle-regulatory genes in plant. It revealed a strong alteration of the expression patterns in the 4x genotypes with respect to the 2x parents. Changes often exceed the twofold, with no consistent trend towards up- or down regulation when comparing 2x vis-à-vis 4x. We discuss the possible relevance of epigenetic changes in controlling the expression of duplicated genes.

Keywords

Chromosome doubling Morpho-anatomical traits Solanum commersonii Solanum bulbocastanum Cell cycle-regulatory genes 

Notes

Acknowledgments

This research was carried out in the frame of the PRIN project “Effects of the ploidy level on gene expression and genome structure in alfalfa and potato” funded by MiUR.

Supplementary material

10681_2014_1338_MOESM1_ESM.docx (15 kb)
Supplementary material 1 (DOCX 14 kb)
10681_2014_1338_MOESM2_ESM.docx (15 kb)
Supplementary material 2 (DOCX 15 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Riccardo Aversano
    • 1
  • Maria-Teresa Scarano
    • 2
  • Giovanna Aronne
    • 1
  • Immacolata Caruso
    • 1
  • Vincenzo D’Amelia
    • 1
  • Veronica De Micco
    • 1
  • Carlo Fasano
    • 1
  • Pasquale Termolino
    • 2
  • Domenico Carputo
    • 1
  1. 1.Department of Agricultural SciencesUniversity of Naples Federico IIPorticiItaly
  2. 2.CNR, Institute of Biosciences and BioResources (IBBR), UOS PorticiPorticiItaly

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