Advertisement

Euphytica

, Volume 203, Issue 3, pp 673–681 | Cite as

Mapping QTLs related to rice seed storability under natural and artificial aging storage conditions

  • Ngo Thi Hang
  • Qiuyun Lin
  • Linglong Liu
  • Xi Liu
  • Shijia Liu
  • Wenyan Wang
  • Linfang Li
  • Niqing He
  • Zhou Liu
  • Ling Jiang
  • Jianmin Wan
Article

Abstract

Seed aging or deterioration in rice (Oryza sativa L.) is a major problem for agronomic production and germplasm preservation. Deciphering the genetic mechanism involved in seed aging and improving seed storability is therefore a vital goal for rice breeding. A set of 85 backcross inbred lines derived from the backcross ‘Sasanishiki’ (japonica cv.)/‘Habataki’ (an indica cv. with strong seed storability)//‘Sasanishiki’ was used here to detect quantitative trait locus (QTL) controlling seed storability. Seeds were treated under natural (conventional storage for 48 months) and artificial aging storage conditions (i.e. increased temperature and relative humidity) and germination rate was used to evaluate seed storability. A total of thirteen QTLs for seed storability were identified on chromosomes 1, 2, 3, 4, 5, 7, 11 and 12, respectively. Among them, two QTLs, viz. qSSh-2-1 and qSSh-2-2, were repeatedly detected in both treatment conditions. In contrast, four (qSSh-4, qSSs-5-1, qSSs-5-2 and qSSh-12) and seven QTLs (qSSh-1, qSSh-3-1, qSSh-3-2, qSSh-3-3, qSSh-7-1, qSSh-7-2 and qSSh-11) were detected only once in natural and artificial aging treatments, respectively. The ‘Habataki’-derived alleles were observed to increase seed storability at all the loci except qSSs-5-1 and qSSs-5-2. The existence of QTLs qSSh-1, qSSh-3-1, qSSh-3-2, qSSh-3-3, qSSh-4, qSSh-7-1, qSSh-7-2 and qSSh-11 was confirmed using Habataki chromosome segment substitution lines in a Sasanishiki genetic background. These results provide an opportunity for map-based cloning of major QTLs for seed storability, thereby gaining an understanding of seed storability in rice and possibilities for its improvement.

Keywords

Rice Seed storability Natural aging Artificial aging Backcross inbred lines Chromosome segment substitution line Quantitative trait locus 

Abbreviations

QTL

Quantitative trait locus

BIL

Backcross inbred line

CSSL

Chromosome segment substitution line

qSSh

Quantitative trait loci of Habataki allele enhancing seed storability

qSSs

Quantitative trait loci of Sasanishiki allele enhancing seed storability

Notes

Acknowledgments

The research described in this paper was financially supported by a project (No. 2014ZX08001-006) from Ministry of Agriculture of China for transgenic research, Key Laboratory of Biology, Genetics and Breeding of Japonica Rice in Mid-lower Yangtse River, Ministry of Agriculture, P.R.China and Jiangsu Qinglan Project.

References

  1. Ando T, Yamamoto T, Shimizu T, Ma XF, Shomura A, Takeuchi Y, Lin SY, Yano M (2008) Genetic dissection and pyramiding of quantitative traits for panicle architecture by using chromosomal segment substitution lines in rice. Theor Appl Genet 116:881–890CrossRefPubMedGoogle Scholar
  2. Chen L (1994) Physiological character of hybrid rice seed and its storage tolerance. Seed 4:19–21Google Scholar
  3. Contreras S, Bennett MA, Metzger JD, Tay D (2008) Maternal light environment during seed development affects lettuce seed weight, germinability, and storability. HortScience 43:845–852Google Scholar
  4. Gan R, Min P, Weijiang T, Caoguo X, Yongzhong X (2005) QTL associated with seed aging in rice. Acta Agronomica Sinica 3:183–187Google Scholar
  5. Kameswara N, Jackson M (1996a) Seed longevity of environment and storage longevity of japonica rices (Oryza sativa L.). Seed Sci Res 6:17–21Google Scholar
  6. Kameswara N, Jackson M (1996b) Seed longevity of rice cultivars and strategies for their conservation in genebanks. Ann Botany 77:251–260CrossRefGoogle Scholar
  7. Li LF, Lin QY, Liu SJ, Liu X, Wang WY, Hang NT, Liu F, Zhao ZG, Jiang L, Wan J (2012) Identification of quantitative trait loci for seed storability in rice (Oryza sativa L). Plant Breeding 131:739–743CrossRefGoogle Scholar
  8. Likhatchev BS, Zelensky GV, Kiashko YG, Shevchenko ZN (1984) Modeling of seed ageing. Seed Science and Technology 12:385–393Google Scholar
  9. Lincoln S, Daly M, Lander E (1993) Constructing genetic linkage maps with MAPMAKER, Version 3: A tutorial and reference manual. Release, Whitehead Institute for Biomedical Research, Cambridge, MAGoogle Scholar
  10. McCouch SR, Teytelman L, Xu YB, Lobos KB, Clare K, Walton M, Fu BY, Maghirang R, Li ZK, Xing YX, Zhang QF, Kono I, Yano M, Fjellstrom R, DeClerck G, Schneider D, Cartinhour S, Ware D, Stein L (2002) Development and mapping of 2240 new SSR marker for rice (Oryza sativa L). DNA Res 9:199–207CrossRefPubMedGoogle Scholar
  11. McCouch SR, Cho YG, Yano M, Paul E, Blinstrub M (1997) Report on QTL nomenclature. Rice Genet Newsl 14:11–13Google Scholar
  12. Miura K, Lin SH, Yano M, Nagamine T (2002) Mapping quantitative trait loci controlling seed longevity in rice (Oryza sativa L.). Theor Appl Genet 104:981–986CrossRefPubMedGoogle Scholar
  13. Oka HI, Tsai KH (1955) Dormancy and longevity of rice seed with regard to their variations among varieties. (in Japanese with English summary). Jpn. J. Breed 5:90–94CrossRefGoogle Scholar
  14. Rajjou L, Debeaujon I (2008) Seed longevity: survival and maintenance of high germination ability of dry seeds. C R Biol 331:796–805CrossRefPubMedGoogle Scholar
  15. Rajjou L, Lovigny Y, Groot SPC, Belghazi M, Job C, Job D (2008) Proteome-wide characterization of seed aging in Arabidopsis: a comparison between artificial and natural aging protocols. Plant Physiol 148:620–641CrossRefPubMedCentralPubMedGoogle Scholar
  16. Sasaki K, Fukuta K, Sato T (2005) Mapping of quantitative trait loci controlling seed longevity of rice (Oryza sativa L.) after various periods of seed storage. Plant Breeding 124:361–366CrossRefGoogle Scholar
  17. Schwember AR, Bradford KJ (2010) Quantitative trait loci associated with longevity of lettuce seeds under conventional and controlled deterioration storage conditions. J Exp Bot 61:4423–4436CrossRefPubMedCentralPubMedGoogle Scholar
  18. Shen S, Zhuang J, Wang S, Yang G, Xia Y (2005) Analysis of QTL with main and epistatic effect of seeds storability in rice. Mol Plant Breed 3:323–328Google Scholar
  19. Specht CE, Keller RJ, Freytag U, Hammer K, Boerner A (1997) Survey of seed germinability after long- term storage in the Gatersleben genebank. Bull. Des Res Phytogenetiques 111:64–68Google Scholar
  20. Specht CE, Freytag U, Hammer K, Boerner A (1998) Survey of seed germinability after long- term storage in the Gatersleben genebank (part 2). Plant Genet Res Newsl 115:39–43Google Scholar
  21. Wang S, Basten C, Zeng Z (2007) Windows QTL cartographer 2.5. Department of Statistics, North Carolina State University, RaleighGoogle Scholar
  22. Wang Z, Wang J, Bao J, Wang F, Zhang H (2010) Quantitative trait loci analysis for rice seed vigor during the germination stage. J Zhejiang Univ Sci B 11:958–964CrossRefPubMedCentralPubMedGoogle Scholar
  23. Xue Y, Zhang S, Yao Q, Peng R, Xiong A, Li X, Zhu W, Zhu Y, Zha D (2008) Identification of quantitative trait loci for seed storability in rice (Oryza sativa L.). Euphytica 164:739–744CrossRefGoogle Scholar
  24. Yamauchi M, Winn T (1996) Rice seed vigor and seeding establishment in anaerobic soil. Crop Sci 36:680–686CrossRefGoogle Scholar
  25. Zeng DL, Qiao Q, Yasukumi K, Teng S, Hiroshi F (2002) Study on storability and morphological index in rice (Oryza sativa L.) under artificial aging. Acta Agron Sin 28:551–554Google Scholar
  26. Zeng DL, Guo LB, Xu YB, Yasukumi K, Zhu LH, Qian Q (2006) QTL analysis of seed storability in rice. Plant Breeding 125:57–60CrossRefGoogle Scholar
  27. Zhang WM, Ni AL, Wang CC (1998) Study on seed vigor of hybrid rice. Seed 2:7–10Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Ngo Thi Hang
    • 1
  • Qiuyun Lin
    • 1
  • Linglong Liu
    • 1
  • Xi Liu
    • 1
  • Shijia Liu
    • 1
  • Wenyan Wang
    • 1
  • Linfang Li
    • 1
  • Niqing He
    • 1
  • Zhou Liu
    • 1
  • Ling Jiang
    • 1
  • Jianmin Wan
    • 1
    • 2
  1. 1.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene EngineeringNanjing Agricultural UniversityNanjingChina
  2. 2.Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijingChina

Personalised recommendations