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Euphytica

, Volume 203, Issue 2, pp 273–283 | Cite as

QTL mapping for salt tolerance based on snp markers at the seedling stage in maize (Zea mays L.)

  • Dezhou Cui
  • Dandan Wu
  • Yamuna Somarathna
  • Chunyan Xu
  • Song Li
  • Peng Li
  • Hua Zhang
  • Huabang Chen
  • Li Zhao
Article

Abstract

Salinity is a major constraint to the sustainability and expansion of maize cultivation. Plant salt tolerance is a quantitative trait controlled by multiple genes. In the present study, we constructed a high density genetic map based on high quality SNP markers from 161 F2:5 recombinant inbred line populations derived from the cross between two maize inbred lines contrasting in salinity tolerance. QTL analysis was conducted in saline field and the hydroponic culture. For saline field, field germination rate and field salt tolerance ranking were used as salinity tolerance indicators to conduct QTL detection. For hydroponic culture, salt tolerance ranking, shoot fresh weight, shoot dry weight, tissue water content, shoot Na+ concentration, shoot K+ concentration, and shoot K+/Na+ ratio were used. Through unconditional QTL analysis, we detected 20 additive and nine epistatic QTLs, of which 12 and two showed significant QTL by treatment (Q × T) interaction effects, respectively. Moreover, the use of conditional analysis model allowed us to detect nine conditional QTLs. The QTLs were mainly clustered on chromosomes 1, 3 and 5. The five unconditional and three conditional QTLs reported here could individually explain more than 20 % of the phenotypic variation. The QTLs identified here could be helpful to improve salt tolerance in maize by marker-assisted selection and shed new light on understanding the genetic basis of salt tolerance in maize.

Keywords

Maize Salt QTL mapping Conditional analysis Seedling stage 

Abbreviations

RIL

Recombinant inbred line

FGR

Field germination rate

FSTR

Field salt tolerance ranking

STR

Salt tolerance ranking

SFW

Shoot fresh weight

SDW

Shoot dry weight

TWC

Tissue water content

SNC

Shoot Na+ concentration

SKC

Shoot K+ concentration

SKN

Shoot K+/Na+ ratio

Q × T

QTL by treatment interaction effects

MAS

Marker-assisted selection

N

Normal treatment

S

160 mM NaCl treatment

Notes

Acknowledgments

The authors thank the China National Science Foundation (Grant No. 31201214) and the National Sci-Tech Support program (Grant No.2013BAD05B01) for providing funds for carrying out this research work. We are very grateful to Dr. Jiping Zhao, Manager-Developmental Biology, Ball Horticultural Company, United States, for thorough English editing of this manuscript.

Supplementary material

10681_2014_1250_MOESM1_ESM.xlsx (34 kb)
Supplementary material 1 (XLSX 33 kb)
10681_2014_1250_MOESM2_ESM.docx (21 kb)
Supplementary material 2 (DOCX 20 kb)
10681_2014_1250_MOESM3_ESM.docx (17 kb)
Supplementary material 3 (DOCX 16 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Dezhou Cui
    • 1
  • Dandan Wu
    • 2
  • Yamuna Somarathna
    • 3
  • Chunyan Xu
    • 1
  • Song Li
    • 1
  • Peng Li
    • 1
  • Hua Zhang
    • 3
  • Huabang Chen
    • 3
  • Li Zhao
    • 3
  1. 1.The State Key Lab of Crop Biology, College of AgricultureShandong Agricultural UniversityTai’anChina
  2. 2.School of Life ScienceShandong UniversityJinanChina
  3. 3.The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijingChina

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