The Sg-6 saponins, new components in wild soybean (Glycine soja Sieb. and Zucc.): polymorphism, geographical distribution and inheritance
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Hypocotyl saponin composition of 1,198 accessions of wild soybean (Glycine soja) collected from China, Korea, Japan and Russia Far East was analyzed by thin-layer chromatography to determine polymorphic variation and geographical distribution. Eight common distinguishable saponin phenotypes were identified: Aa, Ab, AaBc, AbBc, Aa+α, Ab+α, AaBc+α and AbBc+α. The latter four +α type were new. All eight types were identified in China. Type Ab+α was absent in Korea. Types Ab+α and AbBc+α, and Aa+α and Ab+α were not identified in Japan and Russia far east, respectively. Six new triterpene saponins were detected in +α type via LC-PDA/MS/MS analyses. They were, tentatively, designated as H-αg, H-αa, I-αg, I-αa, J-αg and J-αa. These saponins were inherited together by a single dominant allele. A gene symbol Sg-6 was assigned. Hence, the new saponins were collectively named as Sg-6 saponins. The frequency of Sg-6 allele was 17.6 % in Chinese, 10.0 % in Korean and 1.0 % in Japanese wild soybean. The wild soybeans having Sg-6 saponins can be utilized in soybean breeding programs as well as in saponin biosynthesis studies in soybean.
KeywordsGlycine soja Sieb. and Zucc. Soyasaponin polymorphism Triterpene glycosides Wild soybean Soybean
We thank Dr. Sang-Mi Sun for maintaining the germplasm collection. This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0013600).
- Honda N, Tsukamoto C, Maehara Y, Tayama I, Kitamura K, Singh RJ, Chung GH (2009) Proceedings of world soybean research conference VIII, Electronic Press on CD, No Pages, Beijing, ChinaGoogle Scholar
- Krishnamurthy P, Lee CM, Tsukamoto C, Yuya T, Singh RJ, Lee JD, Chung G (2014) Evaluation of genetic structure of Korean wild soybean (Glycine soja) based on saponin allele polymorphism. Genet Resour Crop Evol. doi: 10.1007/s10722-014-0095-4
- Masuda T, Goldsmith PD (2009) World soybean production: area harvested, yield and long-term projections. Int Food Agribus Manag Rev 12:143–162Google Scholar
- Sasama H, Takada Y, Ishimoto M, Kitamura K, Tsukamoto C (2010) Estimation of the mutation site of a soyasapogenol A-deficient soybean [Glycine max (L.) Merr.] by LC-MS/MS profile analysis. In: Cadwallader KR, Chang SKC (eds) Chemistry, texture, and flavor of Soy. Oxford University Press, UK, pp 91–102Google Scholar
- Sayama T, Ono E, Takagi K, Takada Y, Horikawa M, Nakamoto Y, Hirose A, Sasama H, Ohashi M, Hasegawa H, Terakawa T, Kikuchi A, Kato S, Tatsuzaki N, Tsukamoto C, Ishimoto M (2012) The Sg-1 glycosyltransferase locus regulates structural diversity of triterpenoid saponins of soybean. Plant Cell 24:2123–2138PubMedCentralPubMedCrossRefGoogle Scholar
- Sugano M (2006) Soy in health and disease prevention. CRC Press, Taylor and Francis group, New YorkGoogle Scholar
- Takahashi Y, Kon T, Muraoka H, Ishimoto M, Tsukamoto C (2013) The dominant Sg-6 synthesizes saponins with a keton function at oleanane aglycone C-22 position in soybean [Glycine max (L.) Merr.]. 11th international meeting on biosynthesis, function and biotechnology of isoprenoids in terrestrial and marine organisms (TERPNET), Kolymvari, Crete, Greece, p.197Google Scholar
- Tsukamoto C, Yoshiki Y (2006) Soy saponin. In: Sugano M (ed) Soy in health and disease prevention. CRC Press, Taylor and Francis group, New York, pp 155–172Google Scholar