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Euphytica

, Volume 198, Issue 3, pp 413–424 | Cite as

The Sg-6 saponins, new components in wild soybean (Glycine soja Sieb. and Zucc.): polymorphism, geographical distribution and inheritance

  • Panneerselvam Krishnamurthy
  • Chigen Tsukamoto
  • Ram J. Singh
  • Jeong-Dong Lee
  • Hong-Sig Kim
  • Seung-Hwan Yang
  • Gyuhwa Chung
Article

Abstract

Hypocotyl saponin composition of 1,198 accessions of wild soybean (Glycine soja) collected from China, Korea, Japan and Russia Far East was analyzed by thin-layer chromatography to determine polymorphic variation and geographical distribution. Eight common distinguishable saponin phenotypes were identified: Aa, Ab, AaBc, AbBc, Aa+α, Ab+α, AaBc+α and AbBc+α. The latter four +α type were new. All eight types were identified in China. Type Ab+α was absent in Korea. Types Ab+α and AbBc+α, and Aa+α and Ab+α were not identified in Japan and Russia far east, respectively. Six new triterpene saponins were detected in +α type via LC-PDA/MS/MS analyses. They were, tentatively, designated as H-αg, H-αa, I-αg, I-αa, J-αg and J-αa. These saponins were inherited together by a single dominant allele. A gene symbol Sg-6 was assigned. Hence, the new saponins were collectively named as Sg-6 saponins. The frequency of Sg-6 allele was 17.6 % in Chinese, 10.0 % in Korean and 1.0 % in Japanese wild soybean. The wild soybeans having Sg-6 saponins can be utilized in soybean breeding programs as well as in saponin biosynthesis studies in soybean.

Keywords

Glycine soja Sieb. and Zucc. Soyasaponin polymorphism Triterpene glycosides Wild soybean Soybean 

Notes

Acknowledgments

We thank Dr. Sang-Mi Sun for maintaining the germplasm collection. This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0013600).

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Panneerselvam Krishnamurthy
    • 1
  • Chigen Tsukamoto
    • 2
  • Ram J. Singh
    • 3
  • Jeong-Dong Lee
    • 4
  • Hong-Sig Kim
    • 5
  • Seung-Hwan Yang
    • 6
  • Gyuhwa Chung
    • 1
  1. 1.Department of BiotechnologyChonnam National UniversityYeosuKorea
  2. 2.Department of Applied Biological ChemistryIwate UniversityMoriokaJapan
  3. 3.USDA/ARS, National Soybean Research LaboratoryUniversity of IllinoisUrbanaUSA
  4. 4.Division of Plant BiosciencesKyungpook National UniversityDaeguKorea
  5. 5.College of Agriculture, Life and Environment SciencesChungbuk National UniversityCheongjuKorea
  6. 6.Center for Nutraceutical and Pharmaceutical MaterialsMyongji UniversityYonginKorea

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