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Euphytica

, Volume 197, Issue 1, pp 27–37 | Cite as

Comparative analysis of in vitro anther- and isolated microspore culture in hexaploid Triticale (X Triticosecale Wittmack) for androgenic parameters

  • Csaba Lantos
  • Lajos Bóna
  • Krisztina Boda
  • János Pauk
Article

Abstract

Two haploid induction media (190-0 and W14mi) were tested in isolated microspore culture of two triticale (X Triticosecale Wittmack) genotypes. The W14mi medium proved superior for the production of green plantlets in both genotypes. This basic medium (W14) was used to compare two doubled haploid production methods (isolated microspore culture and anther culture) with the same genotypes. The induction of androgenesis was more effective in isolated microspore culture than in anther culture. The number of embryo-like structures was 9.2 times higher in microspore culture (511.0/100 anthers) compared to anther culture (55.5/100 anthers) and the number of regenerant plantlets was also 3.4 times higher (anther culture—20.15/100 anthers; isolated microspore culture—67.6/100 anthers). However, the regenerant plantlets from isolated microspore culture were mainly albinos while predominantly green plantlets were regenerated from anther culture. The production of green plantlets from anther culture (16.8/100 anthers) was 2.9 times higher than from isolated microspore culture (5.8/100 anthers). The efficiency of anther culture was tested with eight winter triticale genotypes. The phenomenon of albinism did not hinder the green plant production in anther culture. Mean green plantlet production was 10.87/100 anthers. This value was two times higher than the number of albinos (5.01/100 anthers) and higher than previously published reports. The anther culture protocol described in this study is an efficient tool for the production of microspore-derived green plantlets in triticale.

Keywords

Androgenesis Anther culture Haploid Microspore culture Triticale X Triticosecale Wittmack 

Abbreviations

DH

Doubled haploid

ELS

Embryo-like structures

Notes

Acknowledgments

The authors thank the conscientious work for Szilvia Palaticki, Ferenc Markó and Ádám Bordé. We would like to acknowledge valuable comments and grammatical corrections made by Sue Broughton (Perth, Australia). This research was realized in the frames of TÁMOP 4.2.4. A/1-11-1-2012-0001 “National Excellence Program—Elaborating and operating an inland student and researcher personal support system” The project was subsidized by the European Union and co-financed by the European Social Fund (Project Number: A1-MZPD-12-0070). This research has been interlocked with GOP-2011-1.1.1 project. The authors thank the suppertment for National Development Agency and Hungarian Economic Development Centre (Project Number: GOP-1.1.1-11-2012-0044).

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Csaba Lantos
    • 1
  • Lajos Bóna
    • 2
  • Krisztina Boda
    • 3
  • János Pauk
    • 1
  1. 1.Department of BiotechnologyCereal Research Non-Profit Limited CompanySzegedHungary
  2. 2.Department of Minor CerealsCereal Research Non-Profit Limited CompanySzegedHungary
  3. 3.Department of Medical Physics and InformaticsUniversity of SzegedSzegedHungary

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