Comparative analysis of in vitro anther- and isolated microspore culture in hexaploid Triticale (X Triticosecale Wittmack) for androgenic parameters
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Two haploid induction media (190-0 and W14mi) were tested in isolated microspore culture of two triticale (X Triticosecale Wittmack) genotypes. The W14mi medium proved superior for the production of green plantlets in both genotypes. This basic medium (W14) was used to compare two doubled haploid production methods (isolated microspore culture and anther culture) with the same genotypes. The induction of androgenesis was more effective in isolated microspore culture than in anther culture. The number of embryo-like structures was 9.2 times higher in microspore culture (511.0/100 anthers) compared to anther culture (55.5/100 anthers) and the number of regenerant plantlets was also 3.4 times higher (anther culture—20.15/100 anthers; isolated microspore culture—67.6/100 anthers). However, the regenerant plantlets from isolated microspore culture were mainly albinos while predominantly green plantlets were regenerated from anther culture. The production of green plantlets from anther culture (16.8/100 anthers) was 2.9 times higher than from isolated microspore culture (5.8/100 anthers). The efficiency of anther culture was tested with eight winter triticale genotypes. The phenomenon of albinism did not hinder the green plant production in anther culture. Mean green plantlet production was 10.87/100 anthers. This value was two times higher than the number of albinos (5.01/100 anthers) and higher than previously published reports. The anther culture protocol described in this study is an efficient tool for the production of microspore-derived green plantlets in triticale.
KeywordsAndrogenesis Anther culture Haploid Microspore culture Triticale X Triticosecale Wittmack
The authors thank the conscientious work for Szilvia Palaticki, Ferenc Markó and Ádám Bordé. We would like to acknowledge valuable comments and grammatical corrections made by Sue Broughton (Perth, Australia). This research was realized in the frames of TÁMOP 4.2.4. A/1-11-1-2012-0001 “National Excellence Program—Elaborating and operating an inland student and researcher personal support system” The project was subsidized by the European Union and co-financed by the European Social Fund (Project Number: A1-MZPD-12-0070). This research has been interlocked with GOP-2011-1.1.1 project. The authors thank the suppertment for National Development Agency and Hungarian Economic Development Centre (Project Number: GOP-1.1.1-11-2012-0044).
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