Comparative analysis of in vitro anther- and isolated microspore culture in hexaploid Triticale (X Triticosecale Wittmack) for androgenic parameters
- 399 Downloads
Two haploid induction media (190-0 and W14mi) were tested in isolated microspore culture of two triticale (X Triticosecale Wittmack) genotypes. The W14mi medium proved superior for the production of green plantlets in both genotypes. This basic medium (W14) was used to compare two doubled haploid production methods (isolated microspore culture and anther culture) with the same genotypes. The induction of androgenesis was more effective in isolated microspore culture than in anther culture. The number of embryo-like structures was 9.2 times higher in microspore culture (511.0/100 anthers) compared to anther culture (55.5/100 anthers) and the number of regenerant plantlets was also 3.4 times higher (anther culture—20.15/100 anthers; isolated microspore culture—67.6/100 anthers). However, the regenerant plantlets from isolated microspore culture were mainly albinos while predominantly green plantlets were regenerated from anther culture. The production of green plantlets from anther culture (16.8/100 anthers) was 2.9 times higher than from isolated microspore culture (5.8/100 anthers). The efficiency of anther culture was tested with eight winter triticale genotypes. The phenomenon of albinism did not hinder the green plant production in anther culture. Mean green plantlet production was 10.87/100 anthers. This value was two times higher than the number of albinos (5.01/100 anthers) and higher than previously published reports. The anther culture protocol described in this study is an efficient tool for the production of microspore-derived green plantlets in triticale.
KeywordsAndrogenesis Anther culture Haploid Microspore culture Triticale X Triticosecale Wittmack
The authors thank the conscientious work for Szilvia Palaticki, Ferenc Markó and Ádám Bordé. We would like to acknowledge valuable comments and grammatical corrections made by Sue Broughton (Perth, Australia). This research was realized in the frames of TÁMOP 4.2.4. A/1-11-1-2012-0001 “National Excellence Program—Elaborating and operating an inland student and researcher personal support system” The project was subsidized by the European Union and co-financed by the European Social Fund (Project Number: A1-MZPD-12-0070). This research has been interlocked with GOP-2011-1.1.1 project. The authors thank the suppertment for National Development Agency and Hungarian Economic Development Centre (Project Number: GOP-1.1.1-11-2012-0044).
- Karsai I, Bedõ Z (1997) Effect of carbohydrate content on the embryoid and plant production in triticale anther culture. Certif Res Commun 20:109–116Google Scholar
- Krzewska M, Czyczylo-Mysza I, Dubas E, Golebiowska-Pikana G, Golemiec E, Stojalowski S, Chrupek M, Zur I (2012) Quantitative trait loci associated with androgenic responsiveness in triticale (X Triticosecale Wittm.) anther culture. Plant Cell Rep 31:2099–2108PubMedCentralPubMedCrossRefGoogle Scholar
- Ouyang JW, Jia SE, Zhang C, Chen X, Fen G (1989) A new synthetic medium (W14) for wheat anther culture. Annual Report, Institute of Genetics, Academia Sinica, Beijing, pp 91–92Google Scholar
- Pauk J, Mihály R, Monostori T, Puolimatka M (2003) Protocol of triticale (X Triticosecale Wittmack) microspore culture. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants: a manual book. Kluwer Academic Publishers, Dordrecht, pp 129–134CrossRefGoogle Scholar
- Ponitka A, Slusarkiewicz-Jarzina A, Wêdzony M, Marciñska I, WoŸna J (1999) The influence of various in vitro culture conditions on androgenetic embryo induction and plant regeneration from hexaploid triticale (X Triticosecale Wittm.). J Appl Genet 40:165–174Google Scholar
- Sozinov A, Lukijanjuk S, Ignatova S (1981) Anther cultivation and induction of haploid plants in triticale. Z Pflanzenzuecht 86:272–285Google Scholar
- Sun CS, Wang CC, Chu CC (1973) Cytological studies on the androgenesis of Triticale. Acta Bot Sin 15:145–154Google Scholar
- Wang YY, Sun CS, Wang CC, Chen NF (1973) The induction of pollen plantlets of Triticale and Capsicum annum from anther culture. Scientia Sin 16:147–151Google Scholar