, Volume 195, Issue 1, pp 69–81 | Cite as

Grain yield and grain protein percentage of common wheat lines with wild emmer chromosome-arm substitutions

  • E. Millet
  • J.-K. Rong
  • C. O. Qualset
  • P. E. Mcguire
  • M. Bernard
  • P. Sourdille
  • M. Feldman


Wild emmer wheat, Triticum turgidum subsp. dicoccoides, (2n = 4× = 28; genome BBAA), the progenitor of domesticated wheat, is genetically closely related to durum and common wheat. This wild taxon has characteristics that would be valuable if transferred to domesticated wheat. A series of chromosome-arm substitution lines (CASLs) of wild emmer wheat were produced in the background of an Israeli common wheat cultivar. These CASLs were evaluated in a pot experiment and in field trials in Israel and California for their grain yield (GY) and its components and for grain protein percentage. In addition, the extent of genetic interactions (epistatic effects) between “wild” and “domesticated” alleles, within and between homoeologous groups 1 and 7 as expressed in grain and protein yields and other quantitative traits, were determined. The research has shown that wild emmer wheat harbors genetic variability for quantitative traits and that the “wild” genes interact among themselves in a non-additive way in the common genetic background. Several chromosome arms improve GY and protein percentage in common wheat, but their effects will be presumably enhanced when combination of genes from several “wild” arms are integrated into a single “domesticated” genotype. Hence, the interaction between these genes and those in the recipient common wheat must be accounted for when higher yield or protein content is desired. The results of this study indicate on the potential of this material for breeding and genetic analysis, and support the idea of pyramiding genes from a wild species.


Common wheat Durum wheat Grain protein content Grain yield Yield components Triticum turgidum subsp. dicoccoides 



This work was supported by United States—Israel Binational Agricultural Research and Development Fund (BARD, Grant No. IS-2578-95) and by the Franco-Israeli Research Cooperation (AFFIRST).


  1. Avivi L (1977) High grain protein content in wild wheat. Can J Gent Cytol 19:569–570Google Scholar
  2. Avivi L (1978) High grain protein content in wild tetraploid Triticum dicoccoides Korn. In: Proceedings of the 5th International Wheat Genetics Symposium, New Delhi, p 372–380Google Scholar
  3. Avivi L (1979) Utilization of Triticum dicoccoides for the improvement of grain protein quantity and quality in cultivated wheats. Monogr Genet Agr 4:27–38Google Scholar
  4. Avivi L, Levy AA, Feldman M (1983) Studies on high protein durum wheat derived from crosses with the wild tetraploid wheat Triticum turgidum var. dicoccoides. In: Proceedings of the 6th International Wheat Genetics Symposium, Kyoto, Japan, p 199–204Google Scholar
  5. Bavec M, Bavec F, Varga B, Kovačević V (2002) Relationships among yield, it’s quality and yield components in winter wheat (Triticum aestivum L.) cultivars affected by seeding rates. Die Bodenkultur 53:143–151Google Scholar
  6. Cantrell RG, Joppa LR (1991) Genetic analysis of quantitative traits in wild emmer (Triticum turgidum L. var. dicoccoides). Crop Sci 31:645–649CrossRefGoogle Scholar
  7. Chao S, Sharp PJ, Worland AJ, Warham EJ, Koebner RMD, Gale MD (1989) RFLP-based genetic map of wheat homoeologous group-7 chromosomes. Theor Appl Genet 78:495–504PubMedCrossRefGoogle Scholar
  8. Chee PW, Elias EM, Anderson JA, Kianian SF (2001) Evaluation of a high grain protein QTL from Triticum turgidum L. var. dicoccoides in an adapted durum wheat background. Crop Sci 41:295–301CrossRefGoogle Scholar
  9. Dubcovsky J, Dvorak J (2007) Genome plasticity a key factor in the success of polyploid wheat under domestication. Science 316:1862–1866PubMedCrossRefGoogle Scholar
  10. Feldman M (1977) Historical aspects and significance of the discovery of wild wheats. Stadler Symposium 9:121–146Google Scholar
  11. Feldman M, Millet E (1991) Utilization of wild tetraploid wheat, Triticum turgidum var dicoccoides, for the increase in yield and protein in cultivated tetraploid and hexaploid wheats. Meeting of the Cereal Section of EUCARPIA, Schwerin (Germany) June 24–27. Vortr Pflanzenzuchtg 20:14–21Google Scholar
  12. Feldman M, Millet E (1995) Methodologies for identification, allocation and transfer of quantitative genes from wild emmer into cultivated wheat. In: Proceedings of the 8th International Wheat Genetics Symposium, Beijing, China, p 19–27Google Scholar
  13. Feldman M, Sears ER (1981) The wild gene resources of wheat. Sci Am 244:102–112CrossRefGoogle Scholar
  14. Feldman M, Avivi L, Levy AA, Zaccai M, Avivi Y, Millet E (1990) High protein wheat. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 6., Crops IISpringer, Berlin, pp 593–614Google Scholar
  15. Feldman M, Millet E, Abbo S (1994) Exploitation of wild emmer wheat to increase yield and protein content in durum and common wheat. In: Proceedings of the EUCARPIA meeting of the Genetic Resources Section, March 15–18, 1994 Clermont-Ferrand, FranceGoogle Scholar
  16. Goldman IL, Paran I, Zamir D (1995) Quantitative trait locus analysis of a recombinant inbred line population derived from a Lycopersicon esculentum × Lycopersicon cheesmanii cross. Theor Appl Genet 90:925–932PubMedCrossRefGoogle Scholar
  17. Haudry A, Cenci A, Ravel C, Bataillon T, Brunel D, Poncet C, Hochu I, Poirier S, Santoni S, Glémin S, David J (2007) Grinding up wheat: a massive loss of nucleotide diversity since domestication. Mol Biol Evol 24:1506–1517PubMedCrossRefGoogle Scholar
  18. Joppa LR (1993) Chromosome engineering in tetraploid wheat. Crop Sci 33:908–913CrossRefGoogle Scholar
  19. Joppa LR, Cantrell RG (1990) Chromosomal location of genes for grain protein content of wild tetraploid wheat. Crop Sci 30:1059–1064CrossRefGoogle Scholar
  20. Joppa LR, Hareland GA, Cantrell RG (1991) Quality characteristics of the Langdon durum-dicoccoides chromosome substitution lines. Crop Sci 31:1513–1517CrossRefGoogle Scholar
  21. Levy AA, Feldman M (1988) Ecogeographical distribution of HMW glutenin alleles in populations of the wild tetraploid wheat Triticum turgidum var. dicoccoides. Theor Appl Genet 75:651–658CrossRefGoogle Scholar
  22. Levy AA, Feldman M (1989) Location of genes for high grain protein percentage and other quantitative traits in wild wheat Triticum turgidum var. dicoccoides. Euphytica 41:113–122CrossRefGoogle Scholar
  23. Levy AA, Galili G, Feldman M (1988) Polymorphism and genetic control of high molecular weight glutenin subunits in wild tetraploid wheat Triticum turgidum var. dicoccoides. Heredity 61:63–72CrossRefGoogle Scholar
  24. Mesfin A, Frohberg RC, Anderson JA (1999) RFLP markers associated with high grain protein from Triticum turgidum L. var. dicoccoides introgressed into hard red spring wheat. Crop Sci 39:508–513CrossRefGoogle Scholar
  25. Millet E, Rong JK, Feldman M (1998) Production of wild emmer recombinant substitution lines in a modern bread wheat cultivar and their use in wheat mapping. Proceedings of the 9th International Wheat Genetics Symposium, vol 1, p 127–130Google Scholar
  26. Millet E, Rong JK, Qualset C, McGuire P, Bernard M, Sourdille P, Feldman M (2012) Production of chromosome-arm substitution lines of wild emmer in common wheat. Euphytica 190:1–17CrossRefGoogle Scholar
  27. Millet E, Rong JK, Qualset C, McGuire P, Bernard M, Sourdille P, Feldman M (2013) Production of wild emmer recombinant substitution lines in a modern common wheat cultivar and their exploitation for wheat improvement. To be submitted to EuphyticaGoogle Scholar
  28. Olmos S, Distelfeld A, Chicaiza O, Schlatter AR, Fahima T, Echenique V, Dubcovsky J (2003) Precise mapping of a locus affecting grain protein content in durum wheat. Theor Appl Genet 107:1243–1251PubMedCrossRefGoogle Scholar
  29. Reif JC, Zhang P, Dreisigacker S, Warburton ML, van Ginkel M, Hoisington D, Bohn M, Melchinger AE (2005) Wheat genetic diversity trends during domestication and breeding. Theor Appl Genet 110:859–864PubMedCrossRefGoogle Scholar
  30. Rong JK, Millet E, Feldman M (1998) A powdery mildew resistance gene from wild emmer transferred into common wheat and tagged by molecular markers. In: Proceedings 9th International Wheat Genetics Symposium vol 3, p 148–15Google Scholar
  31. Rong JK, Millet E, Manisterski J, Feldman M (2000) A new powdery mildew resistance gene: introgession from wild emmer into common wheat and RFLP-based mapping. Euphytica 115:121–126CrossRefGoogle Scholar
  32. SAS User’s Guide (1985) Basics, version 5, edition, Gary NC: SAS Institute, Inc., p 1290Google Scholar
  33. Schwarzbach E (1984) A new approach in the evaluation of field trials: the determination of the most likely genetic ranking of varieties. Vortr. Pfanzenzüchtung, 6:249–259 (Proc Eucarpia Cereal Section Meeting 28. 2–1. 3, Weihhenstephhan)Google Scholar
  34. Slafer GA, Calderini DF, Miralles GJ. 1996. Yield components and compensation in wheat: opportunities for future increasing yield potential. In: Reynolds MP, Rajaram S (eds) Increasing yield potential in wheat: breaking the barriers. Proc. Workshop held in CIMMYT, Ciudad Obregón, Sonora, Mexico, CIMMYT, p 101–133Google Scholar
  35. Uauy C, Distelfeld A, Fahima T, Blechl A, Dubcovsky J (2006) A NAC gene regulating senescence improves grain protein, zinc, and iron content in wheat. Science 314:1298–1301PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • E. Millet
    • 1
    • 2
  • J.-K. Rong
    • 1
    • 3
  • C. O. Qualset
    • 4
  • P. E. Mcguire
    • 4
  • M. Bernard
    • 5
  • P. Sourdille
    • 5
  • M. Feldman
    • 1
  1. 1.Department of Plant SciencesThe Weizmann Institute of ScienceRehovotIsrael
  2. 2.Institute for Cereal Crops ImprovementTel Aviv UniversityTel AvivIsrael
  3. 3.School of Agriculture and Food ScienceZhejiang A & F UniversityHangzhouChina
  4. 4.Department of Plant SciencesUniversity of California DavisDavisUSA
  5. 5.Station d’Amelioration des Plantes, INRAClermont-FerrandFrance

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