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Euphytica

, Volume 192, Issue 2, pp 259–266 | Cite as

The detection of QTLs controlling bacterial wilt resistance in tobacco (N. tabacum L.)

  • Yi-liang Qian
  • Xin-sheng Wang
  • Da-zhou Wang
  • Li-na Zhang
  • Chao-long Zu
  • Zheng-liang Gao
  • Hong-jun Zhang
  • Zhi-yong Wang
  • Xue-yong Sun
  • Da-nian Yao
Article

Abstract

The bacterial tobacco wilt caused by Ralstonia solanacearum is one of the most destructive soil-borne diseases worldwide. One strategy to improve the resistance to bacterial wilt is to make use of plant varieties expressing wilt resistance genes. To characterize the genetics of wilt resistance and to identify relevant molecular markers for use in plant breeding, quantitative trait loci (QTLs) affecting tobacco bacterial wilt resistance were mapped in the F2:3 and F2:4 progeny produced from two crosses between the wilt-resistant breeding lines Enshu and Yanyan97 and the susceptible cultivar TI448A. A linkage map containing 118 loci in 24 linkage groups was constructed for 236 lines from the Enshu×TI448A cross, and a linkage map containing 96 loci in 24 linkage groups was constructed for 264 lines from the Yanyan97×TI448A cross. The wilt resistance of the progeny was examined in field trials conducted in Xuancheng, China, in 2010, 2011, and 2012. The disease severity was assessed on stems using separate rating scales. Mapmaker/EXP 3.0 and Mapmaker/QTL 1.1 were used to identify the qBWR-3a, qBWR-3b, qBWR-5a and qBWR-5b QTLs in linkage group 3 and 5; these four loci were strongly associated with resistance and explain 9.00, 19.70, 17.30, and 17.40 % of the variance in resistance, respectively. The close linkage of the markers PT20275 and PT30229 was detected in both the TI448A×Enshu and TI448A×Yanyan97 crosses, and this linkage group could be used to select individual resistant plants. These findings suggest that one strategy to combat bacterial wilt could be to exploit the resistance genes of the Enshu and Yanyan97 strains.

Keywords

Tobacco Bacterial wilt resistance Quantitative trait loci SSR 

Abbreviations

RLH

Ratio of lesion length to plant height

BWR

Bacterial wilt resistance

MPV

Mid-parent value

QTL

Quantitative trait locus

IM

Interval mapping

SSR

Simple sequence repeats

BSA

Bulk segregation analysis

Notes

Acknowledgments

We would like to thank the Anhui Provincial Corporation of the Chinese National Tobacco Corporation for the financial support of the Tobacco High-Quality-and-Resistance Pyramid Breeding and Bacterial-Wilt-Resistance Genes Analysis project (Grant No. 20100551003).

References

  1. Bindler G, van der Hoeven R, Gunduz I, Plieske J, Ganal M, Rossi L, Gadani F, Donini P (2007) A microsatellite marker based linkage map of tobacco. Theor Appl Genet 114:341–349PubMedCrossRefGoogle Scholar
  2. Danesh D, Aarons S, McGill GE, Young ND (1994) Genetic dissection of oligogenic resistance to bacterial wilt in tomato. Mol Plant-Microbe Interact 7:464–471PubMedCrossRefGoogle Scholar
  3. Fang S, Gu G, Zhang R, Lin H (1997) Preliminary study of new tobacco variety Yanyan97 for bacterial wilt. Fujian Agric Sci Technol 3:16–17Google Scholar
  4. Gillham H (1977) Disease resistant flue-cured tobacco breeding lines for north Queensland. Aust J Exp Agric Animal Husb 17:659–663CrossRefGoogle Scholar
  5. Hayward AC (1991) Biology and epidemiology of bacterial wilt caused by pseudomonas solanacearum. Annu Rev Phytopathol 29:65–87PubMedCrossRefGoogle Scholar
  6. Herselman L, Thwaites R, Kimmins FM, Courtois B, van der Merwe PJA, Seal SE (2004) Identification and mapping of AFLP markers linked to peanut (Arachis hypogaea L.) resistance to the aphid vector of groundnut rosette disease. Theor Appl Genet 109:1426–1433PubMedCrossRefGoogle Scholar
  7. Jesse M, Nagpala PG, Destéfano-Beltrán L, Huang JH, Kim J, Denny T, Cetiner S (1993) Expression of a Cecropin B lytic peptide analog in transgenic tobacco confers enhanced resistance to bacterial wilt caused by pseudomonas solanacearum. Plant Sci 89:43–53CrossRefGoogle Scholar
  8. Kawamura Y, Hase S, Takenaka S, Kanayama Y, Yoshioka H, Kamoun S, Takahashi H (2009) INF1 elicitin activates jasmonic acid and ethylene-mediated signalling pathways and induces resistance to bacterial wilt disease in tomato. J Phytopathol 157:287–297CrossRefGoogle Scholar
  9. La L, Honduras (1964) Biological and physiological aspects of bacterial wilt caused by pseudomonas solanacearum. Annu Rev Phytopathol 2:203–230CrossRefGoogle Scholar
  10. Li Z, Wu S, Bai X, Liu Y, Lu J, Liu Y, Xiao B, Lu X, Fan L (2011) Genome sequence of the tobacco bacterial. J Bacteriol 193:6088PubMedCrossRefGoogle Scholar
  11. Margin B, Thouqet P, Olivier J, Grimsley NH (1999) Temporal and multiple quantitative trait loci analysis of resistance to bacterial wilt in tomato permit resolution of linked loci. Genetics 151:1165–1172Google Scholar
  12. Matsuda T (1977) Fundamental studies on the breeding of bacterial wilt resistant varieties in tobacco (in Japanese). Bull Utsunomiya Tob Exp Stn 15:1–95Google Scholar
  13. Nishi T, Tajima T, Noguchi S, Ajisaka H, Negishi H (2003) Identification of DNA markers of tobacco linked to bacterial wilt resistance. Theor Appl Genet 106:765–770PubMedGoogle Scholar
  14. Pradeepkumar T, Bastian D, Joy M, Radhakrishnan N, Aipe K (2001) Genetic variation in tomato for yield and resistance to bacterial wilt. J Trop Agric 39:157–158Google Scholar
  15. Saghai Maroof MA, Biyashev RM, Yang GP, Zhang Q, Allard RW (1994) Extraordinarily polymorphic microsatellite DNA in barely species diversity, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 91:5466–5570PubMedCrossRefGoogle Scholar
  16. Stephen E, Mark J, Eric S (1993a) Constructing genetic linkage maps with MAPMAKER/EXP Version 3.0. A Whitehead Institute for Bio-medical Research Technical ReportGoogle Scholar
  17. Stephen E, Mark J, Eric S (1993b) Mapping genes controlling quantitative traits using MAPMAKER/QTL Version 1.1. A Whitehead Institute for Bio-medical Research Technical ReportGoogle Scholar
  18. Sun X, Zu C, Gao Z, Ji X, Zhou Y, Yang H, Lili J, Li T (2011) Influence of high plant density on morphology characters and efficacy of resistant identification to bacterial wilt in tobacco. J China Tob 17:1004–5708Google Scholar
  19. Thoquet P, Olivier J, Sperisen C, Rogowsky P, Laterrot H, Grimsley N (1996) Quantitative trait loci determining resistance to bacterial wilt in tomato cultivar Hawaii7996. Mol Plant-Microbe Interact 9:826–836CrossRefGoogle Scholar
  20. Timmerman GM, Frew TJ, Miller AL, Weeden NF, Jermyn WJ (2002) QTL mapping of partial resistance to field epidemics of Ascochyta blight of pea. Crop Sci 42:2100–2111CrossRefGoogle Scholar
  21. Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78PubMedCrossRefGoogle Scholar
  22. Wang JF, Olivier J, Thoquet P, Mangin B, Sauviac L, Grimsley NH (2000) Resistance of tomato line Hawaii7996 to Ralstonia solanacearum Pss4 in Taiwan is controlled mainly by a major strain-specific locus. Mol Plant-Microbe Interact 13:6–13PubMedCrossRefGoogle Scholar
  23. Zhang Q, Sheng BZ, Dai XK, Mei MH, Saghai Marioof MA, Li ZB (1994) Using bulked extremes and recessive class to map genes for photoperiod-susceptible genetic male sterility in rice. Proc Natl Acad Sci 91:8675–8679PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Yi-liang Qian
    • 1
    • 2
  • Xin-sheng Wang
    • 3
  • Da-zhou Wang
    • 3
  • Li-na Zhang
    • 3
  • Chao-long Zu
    • 2
  • Zheng-liang Gao
    • 2
  • Hong-jun Zhang
    • 4
  • Zhi-yong Wang
    • 5
  • Xue-yong Sun
    • 2
  • Da-nian Yao
    • 1
  1. 1.Agricultural College of Anhui Agricultural UniversityHefeiChina
  2. 2.Tobacco Research Institute of Anhui Academy of Agricultural ScienceHefeiChina
  3. 3.Anhui Provincial Corporation of China National Tobacco CorporationHefeiChina
  4. 4.Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural SciencesBeijingChina
  5. 5.Oklahoma State UniversityStillwaterUSA

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