, Volume 191, Issue 3, pp 317–331 | Cite as

QTL mapping for seedling traits under different nitrogen forms in wheat

  • Jin-jie Sun
  • Ying Guo
  • Gui-zhi Zhang
  • Ming-gang Gao
  • Guo-hua Zhang
  • Fan-mei Kong
  • Yan Zhao
  • Si-shen Li


The uptake and utilization of nitrogen (N) by plants are affected by the different forms of N in the soil. In this study, eight morphological traits at the seedling stage were investigated using a set of recombinant inbred lines (RILs). Three hydroponic culture experiments were conducted using three different NO3 /NH4 + ratios (T1 at 50/50 %, T2 at 100/0 % and T3 at 0/100 %). The investigated traits in T2 and T1 were significantly higher than T3 in all three experiments, indicating that NO3 promoted or NH4 + suppressed the production of biomass. Comparing T1 with T2, NO3 significantly increased the values of root fresh weight (RFW), total fresh weight (TFW) and root dry weight (RDW) and significantly decreased shoot dry weight (SDW). A total of 147 quantitative trait loci (QTLs) for the eight traits were detected on 18 chromosomes (except 2A, 3D and 4D). Among them, 16 QTLs (QRfw-1A, QRfw-1D, QSfw-1D, QSfw-2B.1, QTfw-1A, QTfw-1D, QRsfw-2B, QRsfw-3B.1, QRdw-1A.1, QRdw-1A.2, QSdw-6B.1, QSdw-7A.1, QTdw-1A.1, QRsdw-1A.1, QRsdw-5A.1 and QRsdw-7A.1) were detected in more than three of the nine treatment–experiments, and most of the 16 QTLs made large contributions of approximately 15 %. Surprisingly, QRsfw-4A.1 and QRsfw-4A.2 explained as much as 47.9 and 55.5 % of the phenotypic variation, respectively. Thirteen important QTL clusters (C1-C13) with more than four QTLs and involving 66 QTLs (44.9 %) were mapped on chromosomes 1A, 1D, 2B, 2D, 4A, 4B, 5B, 5D, 6B, 7A and 7B.


Triticum aestivum Quantitative trait locus Hydroponic culture Nitrogen (N) Nitrate (NO3Ammonium (NH4+



Root fresh weight


Shoot fresh weight


Total fresh weight




Root dry weight


Shoot dry weight


Total dry weight





This work was supported by the National Key Technologies R&D Program (Grant No. 2011BAD35B03) and the Creation and Utilization of Agriculture–Biology Resources of Shandong Province, China.

Supplementary material

10681_2012_834_MOESM1_ESM.doc (1 mb)
Supplementary material 1 (DOC 1033 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Jin-jie Sun
    • 1
  • Ying Guo
    • 1
    • 2
  • Gui-zhi Zhang
    • 1
  • Ming-gang Gao
    • 1
  • Guo-hua Zhang
    • 1
  • Fan-mei Kong
    • 1
  • Yan Zhao
    • 1
  • Si-shen Li
    • 1
  1. 1.State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop BiologyShandong Agricultural UniversityTai’anChina
  2. 2.Tai’an Academy of Agricultural ScienceTai’anChina

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