Mobilization of the active transposon mPing in interspecific hybrid rice between Oryza sativa and O. glaberrima
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Miniature Ping (mPing) is the first active miniature inverted-repeat transposable element to be identified in rice, and its mobilization is activated by stress treatments. We have examined the mobilization of mPing in four NERICA (New Rice for Africa) lines and 13 interspecific lines. All 17 lines are inbred progenies derived from crosses between Oryza sativa variety WAB56-104 as the recurrent parent and the O. glaberrima variety CG14 as the donor parent. We found that 16 of the 17 lines studied inherited mPing together with its autonomous partner, Pong, from WAB56-104. Transposon display of mPing disclosed polymorphic banding patterns among these lines. Most importantly, seven of the lines displayed clear polymorphic banding patterns for mPing, indicating that mPing might have been mobilized in these lines. Locus-specific PCR analysis also confirmed the mobilization of mPing. These results signify that interspecific hybridization may activate the transposition of mPing. Based on these results, we discuss the potential use of the mPing system as an efficient tool for gene tagging in interspecific hybrid rice.
KeywordsInterspecific cross Interspecific hybrid rice Mobilization mPing Transposon
We thank the WARDA for providing the plant materials of the NERICA and interspecific lines. This study was supported by grants from the Ministry of Education, Culture, Sports and Technology of Japan in the form of Grants-in-Aid for Scientific Research, 21380004.
- Hegarty MJ, Hiscock SJ (2005) Hybrid speciation in plants: new insights from molecular studies. New Phytol 100:735–741Google Scholar
- Jiang N, Wessler SR (2002) Rice non-autonomous DNA transposon STOWAWAY13_OS. Repbase Rep 2:7Google Scholar
- Jones MP, Mande S, Aluko K (1997a) Diversity and potential of Oryza glaberrima Steud. in upland rice breeding. Breed Sci 47:395–398Google Scholar
- Kim CM, Je BI, Piao HL, Park SJ, Kim MJ, Park SH, Park JY, Park SH, Lee EK, Chon NS, Won YJ, Lee GH, Nam MH, Yun DW, Lee MC, Cha YS, Lee KH, Eun MY, Han CD (2002) Reprogramming of the activity of the activator/dissociation transposon family during regeneration in rice. Mol Cells 14:231–237PubMedGoogle Scholar
- Miyao A, Tanaka K, Murata K, Sawaki H, Takeda S, Abe K, Shinozuka Y, Onosato K, Hirochika H (2003) Target site specificity of the Tos17 retrotransposon shows a preference for insertion within genes and against insertion in retrotransposon-rich regions of the genome. Plant Cell 15:1771–1780PubMedCrossRefGoogle Scholar
- Oki N, Okumoto Y, Tsukiyama T, Naito K, Nakazaki T, Tanisaka T (2007) A novel transposon Pyong in the japonica rice variety Gimbozu. Kinki J Crop Sci Breed 52:39–42Google Scholar
- Sarla N, Swamy BPM (2005) Oryza glaberrima: a source for the improvement of Oryza sativa. Curr Sci 89:955–963Google Scholar
- Semagn K, Ndjiondjop MN, Lorieux M, Jones M, McCouch S (2007) Molecular profiling of an interspecific rice population derived from a cross between WAB56-104 (Oryza sativa) and CG 14 (Oryza glaberrima). Afr J Biotechnol 6:2014–2022Google Scholar
- Somado EA, Guei RG, Keya SO (2008) NERICA: the new rice for Africa—a compendium. Pragati Offset, HyderabadGoogle Scholar
- Yano M, Katayose Y, Ashikari M, Yamanouchi U, Monna L, Fuse T, Baba T, Yamamoto K, Umehara Y, Nagamura Y, Sasaki T (2000) Hd1, a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the Arabidopsis flowering time gene CONSTANS. Plant Cell 12:2473–2483PubMedGoogle Scholar