Abstract
Miniature Ping (mPing) is the first active miniature inverted-repeat transposable element to be identified in rice, and its mobilization is activated by stress treatments. We have examined the mobilization of mPing in four NERICA (New Rice for Africa) lines and 13 interspecific lines. All 17 lines are inbred progenies derived from crosses between Oryza sativa variety WAB56-104 as the recurrent parent and the O. glaberrima variety CG14 as the donor parent. We found that 16 of the 17 lines studied inherited mPing together with its autonomous partner, Pong, from WAB56-104. Transposon display of mPing disclosed polymorphic banding patterns among these lines. Most importantly, seven of the lines displayed clear polymorphic banding patterns for mPing, indicating that mPing might have been mobilized in these lines. Locus-specific PCR analysis also confirmed the mobilization of mPing. These results signify that interspecific hybridization may activate the transposition of mPing. Based on these results, we discuss the potential use of the mPing system as an efficient tool for gene tagging in interspecific hybrid rice.
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Acknowledgments
We thank the WARDA for providing the plant materials of the NERICA and interspecific lines. This study was supported by grants from the Ministry of Education, Culture, Sports and Technology of Japan in the form of Grants-in-Aid for Scientific Research, 21380004.
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Yasuda, K., Tsukiyama, T., Karki, S. et al. Mobilization of the active transposon mPing in interspecific hybrid rice between Oryza sativa and O. glaberrima . Euphytica 192, 17–24 (2013). https://doi.org/10.1007/s10681-012-0810-1
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DOI: https://doi.org/10.1007/s10681-012-0810-1