, Volume 190, Issue 1, pp 137–144 | Cite as

Reduced height genes and their importance in winter wheat cultivars grown in southern Russia

  • M. G. Divashuk
  • L. A. Bespalova
  • A. V. Vasilyev
  • I. A. Fesenko
  • O. Yu. Puzyrnaya
  • G. I. Karlov


Reduced height genes are the genetic basis of the “green revolution”. Two agronomically important gibberellic acid (GA)-insensitive genes, Rht-B1b (Rht1) and Rht-D1b (Rht2), localised on chromosomes 4BS and 4DS, respectively, and the GA-responsive gene Rht8, localised on chromosome 2DS, were introduced into many cultivars worldwide. An alternative GA-insensitive gene Rht-B1e (=Rht11) was introduced into Russian wheat cultivars. In this study, we investigated the importance of Rht-B1b, Rht-B1e, Rht-D1b and Rht8 in south Russian bread wheat cultivars. The cultivars were divided into five groups: (1) Rht8c; (2) Rht-B1b; (3) Rht-B1b, Rht8; (4) Rht-B1e, Rht8; and (5) Rht-D1b, Rht8. In the Krasnodar region of south Russia 3,222,321 ha were evaluated for estimating the commercial value of each of these genes in 2009–2011. The results showed that coupling Rht-B1e with Rht8 or Rht-B1b with Rht8 was more successful compared with the effects of other genes or their combinations. The average yield of cultivars carrying Rht-B1e exceeded the average yield of cultivars from the other groups. Our study demonstrates that Rht-B1e can be recommended for use in breeding programs and the presence of a molecular marker for this allele simplifies its transfer to elite wheat germplasm.


Genetic value Semi-dwarf wheat Triticum aestivum 



We thank Dr. Robert McIntosh, University of Sydney, for helpful discussions and checking the English of the manuscript. This work was supported by Grant #14.518.11.7043 from the Russian Ministry of Science and Education.


  1. Allan RE (1989) Agronomic comparisons between Rht 1 and Rht 2 semidwarf genes in winter wheat. Crop Sci 29:1103–1108CrossRefGoogle Scholar
  2. Allan RE, Pritchett JA (1980) Registration of 16 lines of club wheat germplasm. Crop Sci 20:832–833CrossRefGoogle Scholar
  3. Bespalova LA (1982) On the inheritance of the plant height by winter wheat hybrids. Sb. nauch. tr. KNIISH, Krasnodar, pp103-120 (In Russian)Google Scholar
  4. Borlaug NE (1968) Wheat breeding and its impact on world food supply. In: Finlay KW, Sheperd KW (eds) Proceedings of the 3rd International Wheat Genetics Symposium. Australian Academy of Science, Canberra, p 5–15Google Scholar
  5. Borner A, Plaschke J, Korzun V, Worland AJ (1996) The relationships between the dwarfing genes of wheat and rye. Euphytica 89:69–75CrossRefGoogle Scholar
  6. Borrell AK, Incoll LD, Dalling MJ (1991) The influence of the Rht1 and Rht2 alleles on the growth of wheat stems and ears. Ann Bot 67:103–110Google Scholar
  7. Botwright TL, Rebetzke GJ, Condon AG, Richards RA (2001) Influence of variety, seed position and seed source on screening for coleoptile length in bread wheat (Triticum aestivum L.). Euphytica 119:349–356CrossRefGoogle Scholar
  8. Butler JD, Byrne PF, Mohammadi V, Chapman PL, Haley DS (2005) Agronomic performance of Rht alleles in spring wheat population across a range moisture levels. Crop Sci 45:939–947CrossRefGoogle Scholar
  9. Chapman SC, Mathews KL, Trethowan RM, Singh RP (2007) Relationships between height and yield in near-isogenic spring wheats that contrast for major reduced height genes. Euphytica 157:391–397CrossRefGoogle Scholar
  10. Chebotar SV (2008) Allelic characteristics of dwarfing genes in the genepool of winter bread wheats of Ukraine. Genetichni Resursi Roslin 6:96–103 (In Russian)Google Scholar
  11. Chebotar SV, Boerner A, Sivolap YuM (2006) Analysis of dwarfing genes in genotypes of Ukrainian wheat varieties. Cytol Genet 40:12–23Google Scholar
  12. Divashuk MG, Vasilyev AV, Bespalova LA, Karlov GI (2012) Identity of the reduced height genes Rht-11 and Rht-B1e. Russ J Genet 48:761–763CrossRefGoogle Scholar
  13. Ellis MH, Spielmeyer W, Gale KR, Rebetzke GJ, Richards RA (2002) ‘‘Perfect’’ markers for the Rht-B1b and Rht-D1b dwarfing genes in wheat. Theor Appl Genet 105:1038–1042PubMedCrossRefGoogle Scholar
  14. Ellis MH, Rebetzke GJ, Chandler P, Bonnett DG, Spielmeyer W, Richards RA (2004) The effect of different height reducing genes on the early growth of wheat. Funct Plant Biol 31:583–589CrossRefGoogle Scholar
  15. Ellis MH, Bonnett DG, Rebetzke GJ (2007) A 192 bp allele at the Xgwm261 locus is not always associated with Rht8 dwarfing gene in wheat (Triticum aestivum L.). Euphytica 157:209–214CrossRefGoogle Scholar
  16. Evans LT (1998) Feeding the ten billion. Plant and population growth. Cambridge University Press, CambridgeGoogle Scholar
  17. Gale MD, Youssefien S (1985) Dwarfing genes in wheat. In: Russell GE (ed) Progress in plant breeding. Butterworths and Co., London, pp 1–35Google Scholar
  18. Ganeva G, Korzun V, Landjeva S, Tsenov N, Atanasova M (2005) Identification, distribution and effects on agronomic traits of the semi-dwarfing Rht alleles in Bulgarian common wheat cultivars. Euphytica 145:305–315CrossRefGoogle Scholar
  19. Guedira M, Brown-Guedira G, Van Sanford D, Sneller C, Souza E, Marshall D (2010) Distribution of Rht genes in modern and historic winter wheat cultivars from the Eastern and Central USA. Crop Sci 50:1811–1822CrossRefGoogle Scholar
  20. Haque MA, Martinek P, Kobayashi S, Kita I, Ohwaku K, Watanabe N, Kuboyama T (2012) Microsatellite mapping of genes for semi-dwarfism and branched spike in Triticum durum Desf. var. ramosoobscurum Jakubz. “Vetvistokoloskaya”. Genet Resour Crop Evol 59:831–837CrossRefGoogle Scholar
  21. Holzapfel J, Voss H, Miedaner T, Korzun V, Häberle J, Schweizer G, Mohler V, Zimmermann G, Hartl L (2008) Inheritance of resistance to Fusarium head blight in three European winter wheat populations. Theor Appl Genet 117:1119–1128PubMedCrossRefGoogle Scholar
  22. Kertesz Z, Flintham JE, Gale MD (1991) Effects of Rht dwarfing genes on wheat grain yield and its components under Eastern European conditions. Cereal Res Commun 19:297–304Google Scholar
  23. Korzun V, Röder MS, Ganal MW, Worland AJ, Law CN (1998) Genetic analysis of the dwarfing gene (Rht8) in wheat. Part I. Molecular mapping of Rht8 on the short arm of chromosome 2D of bread wheat (Triticium aestivum L.). Theor Appl Genet 96:1104–1109CrossRefGoogle Scholar
  24. Landjeva S, Karceva T, Korzun V, Ganeva G (2011) Seedling growth under osmotic stress and agronomic traits in Bulgarian semi-dwarf wheat: comparison of genotypes with Rht8 and/or Rht-B1 genes. Crop Pasture Sci 62:1017–1025CrossRefGoogle Scholar
  25. Lukyanenko PP, Jogin AF (1974) The use of induced dwarf mutants in wheat breeding. Selekcija i Semenovodstvo 1:13 (In Russian)Google Scholar
  26. Mathews KL, Chapman SC, Trethowan R, Singh RP, Crossa J, Pfeiffer W, van Ginkel M, DeLacy I (2006) Global adaptation of spring bread and durum wheat lines near-isogenic for major reduced height genes. Crop Sci 46:603–613CrossRefGoogle Scholar
  27. McIntosh RA, Yamazaki Y, Devos KM, Dubcovsky J, Rogers WJ, Appels R (2003) Catalogue of gene symbols for wheat. Accessed 09 March 2012
  28. Miralles DJ, Slafer GA (1995) Yield, biomass and yield components in dwarf, semi-dwarf and tall isogenic lines of spring wheat under recommended and late sowing dates. Plant Breed 114:392–396CrossRefGoogle Scholar
  29. Pearce S, Saville R, Vaughan SP, Chandler PM, Wilhelm EP, Sparks CA, Al-Kaff N, Korolev A, Boulton MI, Phillips AL, Hedden P, Nicholson P, Thomas SG (2011) Molecular characterization of Rht-1 dwarfing genes in hexaploid wheat. Plant Physiol 157:1820–1831PubMedCrossRefGoogle Scholar
  30. Rebetzke GJ, Richards RA (2000) Gibberellic acid-sensitive dwarfing genes reduce plant height to increase kernel number and grain yield of wheat. Aust J Argic Res 51:235–245CrossRefGoogle Scholar
  31. Rebetzke GJ, Richards RA, Fischer VM, Mickelson BJ (1999) Breeding long coleoptile, reduced height wheats. Euphytica 106:156–168CrossRefGoogle Scholar
  32. Rebetzke GJ, Bonnett DG, Ellis MH (2012) Combining gibberellic acid-sensitive and insensitive dwarfing genes in breeding of higher-yielding, semi-dwarf wheats. Field Crops Res 127:17–25CrossRefGoogle Scholar
  33. Richards RA (1991) Crop improvement for temperate Australia: future opportunities. Field Crops Res 26:141–169CrossRefGoogle Scholar
  34. Rogowsky PM, Guidet FLY, Langridge P, Shepherd KW, Koebner RMD (1991) Isolation and characterization of wheat-rye recombinants involving chromosome arm 1DS of wheat. Theor Appl Genet 82:537–544CrossRefGoogle Scholar
  35. Šíp V, Chrpova J, Zofajova A, Pankova K, Uzık M, Snape JW (2010) Effects of specific Rht and Ppd alleles on agronomic traits in winter wheat cultivars grown in middle Europe. Euphytica 172:221–233CrossRefGoogle Scholar
  36. Srinivasachary, Gosman N, Steed A, Simmonds J, Leverington-Waite M, Wang Y, Snape J, Nicholson P (2008) Susceptibility to Fusarium head blight is associated with the Rht-D1b semi-dwarfing allele in wheat. Theor Appl Genet 116:1145–1153PubMedCrossRefGoogle Scholar
  37. Tošovic-Maric B, KobiljskI B, Obreht D, Vapa L (2008) Evaluation of wheat rht genes using molecular markers. Genetika 40:3138Google Scholar
  38. Welsh JR, Keim DL, Pirasteh B, Richards RD (1973). Genetic control of photoperiodic response in wheat. In: Sears ER, Sears LMS (eds) Proceedings of the 4th International Wheat Genet Symposium, University of Missouri, Columbia, USA, p 879–884Google Scholar
  39. Worland AJ, Law CN (1985) An effect of temperature on the fertility of wheats containing the dwarfing genes Rht1, Rht2, and Rht3. Annual Report, Plant Breeding Institute, Cambridge, UK, 1984, p 69–71Google Scholar
  40. Worland AJ, Law CN (1986) Genetic analysis of chromosome 2D of wheat. The location of genes affecting height, day length insensitivity and yellow rust resistance. Z Pflanzenzuecht 96:331–345Google Scholar
  41. Worland A, Petrovic S (1988) The gibberellic acid insensitive dwarfing gene from the wheat variety Saitama 27. Euphytica 38:55–63CrossRefGoogle Scholar
  42. Worland AJ, Sayers EJ (1995) Rht1 (B.dw), an alternative allelic variant for breeding semi-dwarf wheat varieties. Plant Breed 114:397–400CrossRefGoogle Scholar
  43. Worland AJ, Law CN, Petrovic S (1988). Pleiotropic effects of the chromosome 2D genes Ppd1, Rht8 and Yr16. In: Miller TE, Koebner RMD (eds) Proceedings of the 7th International Wheat Genetics Symposium, Institute of Plant Science Research, Cambridge, UK, p 669–674Google Scholar
  44. Worland AJ, Law CM, Petrovic S (1990) Height reducing genes and their importance to Yugoslavian winter wheat varieties. Savremena Poljoprivreda 38:245–258Google Scholar
  45. Worland AJ, Börner A, Korzun V, Li WM, Petrovic S, Sayers EJ (1998a) The influence of photoperiod genes on the adaptability of European winter wheat. Euphytica 100:385–394CrossRefGoogle Scholar
  46. Worland AJ, Korzun V, Röder MS, Ganal MW, Law CN (1998b) Genetic analysis of the dwarfing gene Rht8 in wheat. Part II. The distribution and adaptive significance of allelic variants at the Rht8 locus of wheat as revealed by microsatellite screening. Theor Appl Genet 96:1110–1120CrossRefGoogle Scholar
  47. Worland AJ, Sayers EJ, Korzun V (2001) Allelic variation at the dwarfing gene Rht8 locus and its significance in international breeding programmes. Euphytica 119:155–159CrossRefGoogle Scholar
  48. Yan W, Li HB, Cai SB, Ma HX, Rebetzke GJ, Liu CJ (2011) Effects of plant height on type I and type II resistance to Fusarium head blight in wheat. Plant Pathol 60:506–512CrossRefGoogle Scholar
  49. Zhang X, Yang S, Zhou Y, He Z, Xia X (2006) Distribution of the Rht-B1b, Rht-D1b and Rht8 reduced height genes in autumn-sown Chinese wheats detected by molecular markers. Euphytica 152:109–116CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • M. G. Divashuk
    • 1
  • L. A. Bespalova
    • 2
  • A. V. Vasilyev
    • 2
  • I. A. Fesenko
    • 1
  • O. Yu. Puzyrnaya
    • 2
  • G. I. Karlov
    • 1
  1. 1.Centre for Molecular BiotechnologyRussian State Agrarian University–Moscow Timiryazev Agricultural AcademyMoscowRussia
  2. 2.Krasnodar Lukyanenko Research Institute of AgricultureKrasnodarRussia

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