QTL mapping of pod tenderness and total soluble solid in yardlong bean [Vigna unguiculata (L.) Walp. subsp. unguiculata cv.-gr. sesquipedalis]
- 454 Downloads
Yardlong bean [Vigna unguiculata ssp. unguiculata cv.-gr. sesquipedalis] is an important vegetable legume, particularly in Asia. Tenderness and sweetness of fresh pods are the key factors in deciding the commercial acceptance of yardlong bean. We report here for the first time quantitative trait loci (QTL) mapping of these traits from crosses between the yardlong bean accession JP81610 and wild cowpea (V. unguiculata ssp. unguiculata var. spontanea) accession JP89083. Two SSR-based linkage maps developed from BC1F1 [(JP81610 × JP89083) × JP81610] and F2 (JP81610 × JP89083) populations were used for QTL analysis of pod tenderness and total soluble solid (TSS) content. Composite interval mapping (CIM) identified three QTLs for pod tenderness with phenotypic variance explained (PVE) of 5.6–50 % and alleles from JP81610 increased the tenderness. CIM detected two QTLs for pod TSS with PVE of 7 and 9 %, and alleles from JP89083 increased TSS. Locations of these QTLs were compared with those of QTLs controlling domestication-related traits identified in the same populations. All QTLs for pod tenderness co-localized with QTLs for pod length. QTLs for pod TSS co-located with QTLs for pod dehiscence and/or pod length. The implications of these QTLs in breeding new yardlong bean and cowpea cultivars are discussed.
KeywordsYardlong bean Cowpea Pod quality Pod tenderness Total soluble solid
This study was financially supported by the Royal Golden Jubilee Ph.D. Program of the Thailand Research Fund (TRF).
- Chetelat RT, De Verna JW, Bennett AB (1995) Introgression into tomato (Lycopersicon esculentum) of the L. chmielewskii sucrose accumulator gene (sucr) controlling fruit sugar composition. Theor Appl Genet 91(2):327–333Google Scholar
- Kader AA (1996) Maturity, ripening, and quality relationships of fruit-vegetable. Acta Hort 434:249–256Google Scholar
- Kearsey MJ, Pooni HS (1956) The genetical analysis of quantitative traits. Chapman & Hall, LondonGoogle Scholar
- Pérez-Alfocea F, Balibrea ME, Bolarín MC, Cuartero J (1997) Efecto de la salinidad sobre el rendimiento y la calidad del fruto en Lycopersion esculentum, L. pimpinellifolium y en sus híbridos interspecíficos. Acta Hortic 16:243–247Google Scholar
- Pornsuriya P (1994) Comparison and inheritance of pod quality in crosses between yardlong bean and cowpea. MS Thesis, Kasetsart University, BangkokGoogle Scholar
- R Development Core Team (2010) R: A language and environment for statistical computing. R Foundation for statistical computing, ViennaGoogle Scholar
- Singh BB (2005) Cowpea [Vigna unguiculata (L.) Walp.]. In: Singh RJ, Jauhar PP (eds.) Genetic resources chromosome engineering and crop improvement. Taylor & Francis, LLC, pp 117–161Google Scholar
- Somta P, Kaga A, Tomooka N, Kashiwaba K, Isemura T, Chaitieng B, Srinives P, Vaughan DA (2006) Development of an interspecific Vigna linkage map between Vigna umbellata (Thunb.) Ohwi & Ohashi and V. nakashimae (Ohwi) Ohwi & Ohashi and its use in analysis of bruchid resistance and comparative genomics. Plant Breed 125:77–84CrossRefGoogle Scholar
- Steele WM, Mehra KL (1980) Structure, evolution, and adaptation to farming systems and environments in Vigna. In: Summerfield RJ, Bunting AH (eds) Advances in Legume Sciences. Royal Botanic Gardens, Kew, pp 393–404Google Scholar
- Wang S, Basten C, Zeng Z-B (2007) Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, RaleighGoogle Scholar