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Euphytica

, Volume 189, Issue 2, pp 217–223 | Cite as

QTL mapping of pod tenderness and total soluble solid in yardlong bean [Vigna unguiculata (L.) Walp. subsp. unguiculata cv.-gr. sesquipedalis]

  • Alisa Kongjaimun
  • Prakit Somta
  • Norihiko Tomooka
  • Akito Kaga
  • Duncan A. Vaughan
  • Peerasak Srinives
Article

Abstract

Yardlong bean [Vigna unguiculata ssp. unguiculata cv.-gr. sesquipedalis] is an important vegetable legume, particularly in Asia. Tenderness and sweetness of fresh pods are the key factors in deciding the commercial acceptance of yardlong bean. We report here for the first time quantitative trait loci (QTL) mapping of these traits from crosses between the yardlong bean accession JP81610 and wild cowpea (V. unguiculata ssp. unguiculata var. spontanea) accession JP89083. Two SSR-based linkage maps developed from BC1F1 [(JP81610 × JP89083) × JP81610] and F2 (JP81610 × JP89083) populations were used for QTL analysis of pod tenderness and total soluble solid (TSS) content. Composite interval mapping (CIM) identified three QTLs for pod tenderness with phenotypic variance explained (PVE) of 5.6–50 % and alleles from JP81610 increased the tenderness. CIM detected two QTLs for pod TSS with PVE of 7 and 9 %, and alleles from JP89083 increased TSS. Locations of these QTLs were compared with those of QTLs controlling domestication-related traits identified in the same populations. All QTLs for pod tenderness co-localized with QTLs for pod length. QTLs for pod TSS co-located with QTLs for pod dehiscence and/or pod length. The implications of these QTLs in breeding new yardlong bean and cowpea cultivars are discussed.

Keywords

Yardlong bean Cowpea Pod quality Pod tenderness Total soluble solid 

Notes

Acknowledgments

This study was financially supported by the Royal Golden Jubilee Ph.D. Program of the Thailand Research Fund (TRF).

Supplementary material

10681_2012_781_MOESM1_ESM.docx (17 kb)
Supplementary material 1 (DOCX 17 kb)

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Alisa Kongjaimun
    • 1
  • Prakit Somta
    • 1
  • Norihiko Tomooka
    • 2
  • Akito Kaga
    • 2
  • Duncan A. Vaughan
    • 2
  • Peerasak Srinives
    • 1
  1. 1.Department of Agronomy, Faculty of Agriculture at Kamphaeng SaenKasetsart UniversityNakhon PathomThailand
  2. 2.Genetic Resources CenterNational Institute of Agrobiological SciencesTsukubaJapan

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