, Volume 190, Issue 1, pp 87–97 | Cite as

Association of staygreen trait with canopy temperature depression and yield traits under terminal heat stress in wheat (Triticum aestivum L.)

  • Maya Kumari
  • R. N. Pudake
  • V. P. Singh
  • Arun K. Joshi


The presence or absence of the staygreen trait was screened for 3 consecutive years in 963 wheat lines from various sources, including Indian and CIMMYT germplasm. Staygreen was assessed at the late dough stage by visual scoring (0–9 scale) and the leaf area under greenness (LAUG) measurement. Around 5.5 % of the lines were staygreen, 10.5 % were moderately staygreen, and the remaining lines showed little or no expression of the trait. One hundred lines showing diversity for the staygreen trait were sown under three different sowing dates (timely, late and very late) for 3 consecutive years in three replications to determine the association of staygreen with heat tolerance. There was a decline in yield, biomass, grain filling duration (GFD) and 1,000 grain weight (TGW) under late and very late sowing conditions owing to terminal stress at anthesis and later stages. However, the decline was relatively less in staygreen genotypes compared to the non-staygreen (NSG) ones. The correlation study showed that LAUG and canopy temperature depression (CTD) were strongly correlated. LAUG and CTD were also significantly associated with grain yield, GFD and biomass. To further confirm the association of the staygreen trait with terminal heat stress, individual F2-derived F7 progenies from the cross of the ‘staygreen’ lines with NSG were evaluated for yield and yield traits at the three sowing dates. In each cross, the staygreen progenies showed a significantly smaller decline in yield and TGW under heat stress than the NSG progenies. These results appear to suggest an association between the staygreen trait and terminal heat stress and, thereby, that the staygreen trait could be used as a morphological marker in wheat to screen for heat tolerance.


Staygreen CTD Terminal heat stress Wheat Triticum aestivum 



The financial support provided by the Council of Scientific and Industrial Research (CSIR), New Delhi, India that enabled this research work to be carried out is gratefully acknowledged. We thank Dr. J. Crossa, Head, Biometrics and Statistics Unit, International Maize and Wheat Improvement Center (CIMMYT), Mexico and Dr. Rajender Prasad, Indian Statistical Research Institute, New Delhi, India for their help in the data analysis.

Supplementary material

10681_2012_780_MOESM1_ESM.docx (139 kb)
Supplementary material 1 (DOCX 139 kb)


  1. Ahlawat S, Chhabra AK, Behl RK, Bisht SS (2008) Genotypic divergence analysis for stay green characters in wheat (Triticum aestivum L. em. Thell). South Pac J Nat Sci 26(1):):73–81CrossRefGoogle Scholar
  2. Al-Khatib K, Paulsen GM (1990) Photosynthesis and productivity during high temperature stress of wheat genotypes from major world regions. Crop Sci 30:1127–1132CrossRefGoogle Scholar
  3. Amani I, Fischer RA, Reynolds MP (1996) Canopy temperature depression association with yield of irrigated spring wheat cultivars in hot climate. J Agron Crop Sci 176:119–129CrossRefGoogle Scholar
  4. Borrel AK, Tao YZ, McIntyre CL (2000) Physiological basis, QTL and MAS of staygreen drought resistance trait in grain sorghum. In: Ribaut JM, Polard D (eds) Molecular approaches for the genetic improvement of cereals for stable production in water limited environments. A strategic planning workshop. CIMMYT, EI Batan, pp 142–146Google Scholar
  5. Ceppi D, Sala M, Gentinetta E, Verderio A, Motto M (1987) Genotypic dependent leaf senescence in maize. Plant Physiol 85:720–725PubMedCrossRefGoogle Scholar
  6. Chen J, Liang Y, Hu X, Wang X, Tan F, Zhang H, Ren Z, Luo P (2010) Physiological characterization of ‘stay green’ wheat cultivars during the grain filling stage under field growing conditions. Acta Physiol Plant 32(5):875–882CrossRefGoogle Scholar
  7. Christopher JT, Manschadi AM, Hammer GL, Borrell AK (2008) Staygreen wheat for Australia’s changing dry environment. In: Appels R, Eastwood R, Lagudah E, Langridge P, Mackay M, McIntyre L, Sharp P (eds) 11th International wheat genetics symposium 2008—Proceedings, vol 1. Sydney University Press, Sydney, pp 119–120Google Scholar
  8. Duvick DN (1984) Genetic contribution to yield gains of US hybrid maize 1930–1980. In: Fehr WR (ed) Genetic contributions to yield gains of five major crop plants (CSSA special publication 7). Crop Science Society of America, Madison, pp 15–45Google Scholar
  9. Evangelista CC, Tangonan NG (1990) Reaction of 31 non-senescent sorghum genotypes to stalk rot complex in Southern Philippines. Trop Pest Manag 36:214–215CrossRefGoogle Scholar
  10. Fischer RA, Byerlee DB (1991) Trends of wheat production in the warmer areas: major issues and economic considerations. In: Wheat for the Non-traditional Warm Areas. Proceeding conference. CIMMYT, Iguazu, pp 3–27Google Scholar
  11. Fischer RA, Rees D, Sayre KD, Lu ZM, Condon AG, Saavedra AL (1998) Wheat yield progress associated with higher stomatal conductance and photosynthetic rate, and cooler canopies. Crop Sci 38:1467–1475CrossRefGoogle Scholar
  12. Gentinetta E, Ceppi D, Lepori C, Perico G, Motto M, Salamini F (1986) A major gene for delayed senescence in maize. Pattern of photosynthates accumulation and inheritance. Plant Breed 97:193–203CrossRefGoogle Scholar
  13. Gong YH, Zhang J, Gao JF, Lu JY, Wang JR (2005) Slow export of photoassimilate from stay-green leaves during late grain-filling stage in hybrid winter wheat (Triticum aestivum L.). J Agron Crop Sci 191(4):292–299CrossRefGoogle Scholar
  14. Gorny AG, Garczynski S (2002) Genotypic and nutrition-dependent variation in water use efficiency and photosynthetic activity of leaves in winter wheat (Triticum aestivum L.). J Appl Genet 43(2):145–160PubMedGoogle Scholar
  15. Gregersen PL, Holm PB, Krupinska K (2008) Leaf senescence and nutrient remobilisation in barley and wheat. Plant Biol 10(1):37–49PubMedCrossRefGoogle Scholar
  16. Ismail AM, Hall AE, Ehlers JD (2000) Delayed leaf senescence and heat tolerance traits mainly are independently expressed in cowpea. Crop Sci 40:1049–1055CrossRefGoogle Scholar
  17. Jiang GH, He CG, Xu CG, Li XH, Zhang Q (2004) The genetic basis of staygreen in rice analyzed in a population of doubled haploid lines derived from an indica by japonica cross. Theor Appl Genet 108:688–698PubMedCrossRefGoogle Scholar
  18. Joshi AK, Chand R, Arun B (2002) Relationship of plant height and days to maturity with resistance to spot blotch in wheat. Euphytica 123:221–228Google Scholar
  19. Joshi AK, Chand R, Arun B, Singh RP, Ortiz R (2007a) Breeding crops for reduced-tillage management in the intensive, rice-wheat systems of South Asia. Euphytica 153(1–2):135–151Google Scholar
  20. Joshi AK, Kumari M, Singh VP, Reddy CM, Kumar S, Rane J, Chand R (2007b) Staygreen trait: variation, inheritance and its association with spot blotch resistance in spring wheat (Triticum aestivum L.). Euphytica 153(1–2):59–71Google Scholar
  21. Kohli MM, Mann CE, Rajaram S (1991) Global state and recent progress in breeding wheat for the warmer areas. In: Saundres DA (ed) Wheat for nontraditional, warm areas. CIMMYT, Mexico, pp 96–112Google Scholar
  22. Kumar U, Joshi AK, Kumari M, Paliwal R, Kumar S, Röder MS (2010) Identification of QTLs for stay green trait in wheat (Triticum aestivum L.) in the ‘Chirya 3’ × ‘Sonalika’ population. Euphytica 174(5):437–445CrossRefGoogle Scholar
  23. Phillips DA, Pierce RO, Edie SA, Foster KW, Knowles PF (1984) Delayed leaf senescence in soybean. Crop Sci 24:518–522CrossRefGoogle Scholar
  24. Rawson HM, Hindmarsh JH, Fischer RA, Stockman YM (1983) Changes in leaf photosynthesis with plant ontogeny and relationships with yield per ear in wheat cultivars and 120 progeny. Aust J Plant Physiol 10:503–514CrossRefGoogle Scholar
  25. Rehman A, Habib I, Ahmad N, Hussain M, Khan MA, Farooq J, Ali MA (2009) Screening wheat germplasm for heat tolerance at terminal growth stage. Plant Omics 2(1):9–19Google Scholar
  26. Reynolds MP (2002) Physiological approaches to wheat breeding. In: Curtis BC, Rajaram S, Gomez Macpherson H (eds) Bread wheat: improvement and production. Food and Agriculture Organization, RomeGoogle Scholar
  27. Reynolds MP, Bolota M, Delgado MIB, Amani I, Fischer RA (1994) Physiological and morphological traits associated with spring wheat yield under hot, irrigated conditions. Aust J Plant Physiol 21:717–730CrossRefGoogle Scholar
  28. Reynolds MP, Singh RP, Ibrahim OA, Ageeb A, Quick JS (1998) Evaluating physiological traits to complement empirical selection for wheat in warm environments. Euphytica 100:84–95CrossRefGoogle Scholar
  29. Reynolds MP, Nagarajan S, Razzaque MA, Ageeb OAA (2001) Breeding for adaptation to environmental factors: heat tolerance. In: Reynolds MP, Ortiz-Monasterio JI, McNab A (eds) Application of physiology in wheat breeding. CIMMYT, Mexico, pp 124–135Google Scholar
  30. Rosenow DT (1987) Breeding sorghum for drought resistance. In: Menyonga JM, Bezuneh T, Youdeowei A (ed) Proceedings of International Drought Symposium. Food and grain production in semi arid Africa, Nairobi, pp 83–89Google Scholar
  31. Rosenow DT (1994) Evaluation for drought and disease resistance in sorghum for use in molecular marker assisted selection. In: Witcombe JR, Duncan RR (eds) Proceeding conference: Use of molecular markers in sorghum and pearl millet breeding for developing countries. Norwich, pp 27–31Google Scholar
  32. Rosenow DT, Quisenberry JE, Wendt CW, Clark LE (1983) Drought tolerant sorghum and cotton germplasm. Agric Water Manag 7:207–222CrossRefGoogle Scholar
  33. Russel WA (1986) Contribution of breeding maize improvement in the United States, 1920–198s. Iowa State. J Res 61:5–34Google Scholar
  34. SAS Institute (2003) SAS user’s guide. Statistics. SAS Institute, CaryGoogle Scholar
  35. Silva SA, Carvallo FIF, Caetano VR, Oliveira AC, Coimbra JLM, Vasconcellos NJS, Lorencetti C (2000) Genetic basis of stay-green trait. J New Seeds 2(2):55–68CrossRefGoogle Scholar
  36. Singh RP, Rajaram S (1992) Genetics of adult-plant resistance to leaf rust in ‘Frontana’ and three CIMMYT wheats. Genome 35:24–31CrossRefGoogle Scholar
  37. Spano G, Di Fonzo N, Perrotta C, Platani C, Ronga G, Lawlor DW, Napier JA, Shewry PR (2003) Physiological characterization of ‘staygreen’ mutants in durum wheat. J Exp Bot 54:1415–1420PubMedCrossRefGoogle Scholar
  38. Thomas H, Howarth CJ (2000) Five ways to staygreen. J Exp Bot 51:329–337PubMedCrossRefGoogle Scholar
  39. Thorne GN (1982) Distribution between parts of the main shoot and the tillers of photosynthate produced before and after anthesis in the top three leaves of the main shoot of Hobbit and Maris Huntsman winter wheat. Ann Appl Biol 101:553–559CrossRefGoogle Scholar
  40. Vietor DM, Cralle HT, Miller FR (1989) Partitioning of 14C-photosynthate and biomass in relation to senescence characteristics of sorghum. Crop Sci 29:1049–1053CrossRefGoogle Scholar
  41. Vijayalakshmi K, Fritz AK, Paulsen GM, Bai G, Pandravada S, Gill BS (2010) Modeling and mapping QTL for senescence-related traits in winter wheat under high temperature. Mol Breed 26(2):163–175CrossRefGoogle Scholar
  42. Walulu RS, Rosenow DT, Wester DR, Nguyen HT (1994) Inheritance of the staygreen trait in sorghum. Crop Sci 34:970–972CrossRefGoogle Scholar
  43. Xu W, Rosenow DT, Nguyen HT (2000) Staygreen trait in grain sorghum: relationship between visual rating and leaf chlorophyll concentration. Plant Breed 119:365–367CrossRefGoogle Scholar
  44. Zadoks JC, Chang TT, Konzak CR (1974) A decimal code for the growth stages of cereals. Weed Res 14:415–421CrossRefGoogle Scholar
  45. Zhang CJ, Chen GX, Gao XX, Chu CJ (2006) Photosynthetic decline in flag leaves of two field-grown spring wheat cultivars with different senescence properties. South African J Bot 72:15–23Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Maya Kumari
    • 1
    • 2
  • R. N. Pudake
    • 3
  • V. P. Singh
    • 1
  • Arun K. Joshi
    • 1
    • 4
  1. 1.Department of Genetics and Plant Breeding, Institute of Agricultural SciencesBanaras Hindu UniversityVaranasiIndia
  2. 2.Molecular Biology and Genetic EngineeringDefence Institute of Bio-Energy ResearchHaldwaniIndia
  3. 3.Department of Biological SciencesGovind Ballabh Pant University Agriculture & TechnologyPantnagarIndia
  4. 4.CIMMYT South AsiaKathmanduNepal

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