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SSR markers linked to kernel weight and tiller number in sorghum identified by association mapping

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Abstract

Sorghum is an energy crop with high biomass production potential and low input requirement. To identify markers linked to grain and biomass production traits, 43 SSR markers were mapped for association with tiller number and kernel weight using the sorghum mini core of 242 landraces. While kernel weight was evaluated in two environments, tiller number was evaluated in four environments. The number of SSR alleles was positively correlated with polymorphism information content for the markers. Association mapping found one marker (4-162) linked to kernel weight and two (40-1896 and 81-108) to tiller numbers. 4-162 and 40-1896 co-localized with previously mapped quantitative trait loci. Localized association mapping around 81-108 identified an amino-cyclopropane-carboxylate (ACC) oxidase gene as a candidate for tiller number. ACC oxidase is an ethylene forming enzyme and increased ethylene level has been shown to increase the number of tillers in the grass family. The results provide the groundwork to identify genes regulating kernel weight and tiller number in sorghum in the future.

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Acknowledgments

This study was supported by the University of Louisiana at Lafayette and the International Crops Research Institute for the Semi-Arid Tropics. We thank the reviewers for their critical comments to improve the manuscript.

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Authors and Affiliations

  1. Gene Bank, International Crops Research Institute for the Semi Arid Tropics (ICRISAT), Patancheru, 502 324, Andhra Pradesh, India

    Hari D. Upadhyaya, Shivali Sharma & Sube Singh

  2. Department of Biology, University of Louisiana at Lafayette, Lafayette, LA, 70504, USA

    Yi-Hong Wang & Karl H. Hasenstein

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  1. Hari D. Upadhyaya
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Correspondence to Yi-Hong Wang.

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Hari D. Upadhyaya and Yi-Hong Wang contributed equally to this work.

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Upadhyaya, H.D., Wang, YH., Sharma, S. et al. SSR markers linked to kernel weight and tiller number in sorghum identified by association mapping. Euphytica 187, 401–410 (2012). https://doi.org/10.1007/s10681-012-0726-9

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