Analysis of QTL for yield-related traits in cassava using an F1 population from non-inbred parents
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A cassava F1 population raised from the cross SC6 × Mianbao was used to construct a genetic linkage map. The map incorporated 200 polymorphic amplified fragment length polymorphism, sequence-related amplified polymorphism, simple sequence repeat (SSR), and expressed sequence tag (EST)–SSR markers which fit a 1:1 segregation ratio. It comprised 20 linkage groups (LGs) and spanned a genetic distance of 1645.1 cM with an average marker interval of 8.2 cM. Fifty-seven repeatedly detected QTLs (rd-QTLs) for three phenotypic traits (fresh root yield, root dry matter content, and root starch content) were identified in the F1 population in four trials of year 2003, 2004, 2005, and 2008 by inclusive composite interval mapping. Among the 57 rd-QTLs, 25 rd-QTLs were linked to SSR/EST–SSR markers, which will help to facilitate marker-assisted selective breeding in cassava, and 15 marker intervals on ten LGs showed pleiotropic effects.
KeywordsCassava Genetic map Breeding
This work was supported financially by the National Basic Research and Development Program (2010CB126600) and the earmarked fund for China Agriculture Research System (CARS-12). We are grateful to Martin Fregene (the Director of Biocassava Plus at the Donald Danforth Plant Science Center, formerly of CIAT) for his gift of the genomic SSR primer pairs.
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