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Euphytica

, Volume 187, Issue 2, pp 227–234 | Cite as

Analysis of QTL for yield-related traits in cassava using an F1 population from non-inbred parents

  • Xin Chen
  • Yuhua Fu
  • Zhiqiang Xia
  • Li Jie
  • Haiyan Wang
  • Cheng Lu
  • Wenquan Wang
Article

Abstract

A cassava F1 population raised from the cross SC6 × Mianbao was used to construct a genetic linkage map. The map incorporated 200 polymorphic amplified fragment length polymorphism, sequence-related amplified polymorphism, simple sequence repeat (SSR), and expressed sequence tag (EST)–SSR markers which fit a 1:1 segregation ratio. It comprised 20 linkage groups (LGs) and spanned a genetic distance of 1645.1 cM with an average marker interval of 8.2 cM. Fifty-seven repeatedly detected QTLs (rd-QTLs) for three phenotypic traits (fresh root yield, root dry matter content, and root starch content) were identified in the F1 population in four trials of year 2003, 2004, 2005, and 2008 by inclusive composite interval mapping. Among the 57 rd-QTLs, 25 rd-QTLs were linked to SSR/EST–SSR markers, which will help to facilitate marker-assisted selective breeding in cassava, and 15 marker intervals on ten LGs showed pleiotropic effects.

Keywords

Cassava Genetic map Breeding 

Notes

Acknowledgments

This work was supported financially by the National Basic Research and Development Program (2010CB126600) and the earmarked fund for China Agriculture Research System (CARS-12). We are grateful to Martin Fregene (the Director of Biocassava Plus at the Donald Danforth Plant Science Center, formerly of CIAT) for his gift of the genomic SSR primer pairs.

Supplementary material

10681_2012_662_MOESM1_ESM.xls (93 kb)
Supplementary material 1 (XLS 93 kb)

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Xin Chen
    • 1
    • 2
  • Yuhua Fu
    • 1
    • 2
  • Zhiqiang Xia
    • 1
    • 2
  • Li Jie
    • 1
    • 2
  • Haiyan Wang
    • 1
  • Cheng Lu
    • 1
    • 2
  • Wenquan Wang
    • 1
    • 2
  1. 1.Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural Sciences (CATAS)HaikouPeople’s Republic of China
  2. 2.Key Laboratory of Biology and Genetic Resources of Tropical CropsMinistry of AgricultureHaikouPeople’s Republic of China

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