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Molecular detection of rye (Secale cereale L.) chromatin in wheat line 07jian126 (Triticum aestivum L.) and its association to wheat powdery mildew resistance

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Abstract

Powdery mildew (Pm), caused by Blumeria graminis f. sp. tritici (Bgt), is one of the most serious diseases for common wheat in many regions around the world. Seeking for new resistance source is urgently required to meet the challenge of the rapid loss of resistance due to the co-evolution of the pathogen’s virulence. Wheat line 07jian126 (Triticum aestivum L.) is highly resistant to the Pm disease prevailing in Sichuan province of China. Previous study showed that a SSR marker Xbarc183 was linked to the Pm resistance in 07jian126, which might be controlled by a single dominant gene, designated as Pm07J126. In this study, two additional F2 populations were used to confirm the linkage between Pm07J126 and Xbarc183. Furthermore, rye chromatin was detected in 07jian126 by molecular analysis of a rye-specific SCAR marker O5 which co-segregated with Pm07J126. This result indicated that Pm07J126 might originate from rye. The reaction patterns to 21 Bgt isolates and molecular marker analysis implied that Pm07J126 might be different from the known rye-derived Pm genes Pm7, Pm8, Pm17 and PmJZHM2RL. Chromosome observation, molecular marker, and A-PAGE analysis suggested that 07jian126 might be a rye introgression line and neither contain 1RS translocation nor secalins gene. Consequently, 07jian126 could be considered as a valuable resource for Pm resistance development of wheat. Besides, the molecular markers Xbarc183 and O5 are useful in marker-assisted selection of Pm07J126 in wheat breeding programs.

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Acknowledgments

This work was supported by Main Direction Program of Knowledge Innovation of Chinese Academy of Sciences (No. KSCX3-EW-N-02-2) and Key Technology R&D Program of Sichuan province.

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Authors and Affiliations

  1. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China

    Shuiyang Yu, Hai Long, Jie Zhang, Guangbing Deng, Zhifen Pan & Maoqun Yu

  2. College of Life Science, Sichuan Normal University, Chengdu, 610101, China

    Shuiyang Yu & Erliang Zhang

  3. Horticultural Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, P.R. China

    Hong Yang

  4. Graduate University of the Chinese Academy of Sciences, Beijing, 100039, China

    Jie Zhang

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  1. Shuiyang Yu
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  2. Hai Long
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  5. Guangbing Deng
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Correspondence to Erliang Zhang or Maoqun Yu.

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Yu, S., Long, H., Yang, H. et al. Molecular detection of rye (Secale cereale L.) chromatin in wheat line 07jian126 (Triticum aestivum L.) and its association to wheat powdery mildew resistance. Euphytica 186, 247–255 (2012). https://doi.org/10.1007/s10681-012-0649-5

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