Molecular mapping of partial resistance to powdery mildew in winter wheat cultivar Folke
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The Swedish winter wheat (Triticum aestivum L.) cultivar Folke has a long record of partial and race non-specific resistance to powdery mildew (caused by Blumeria graminis f. sp. tritici) in the field. The aim of the present study was to map the main genetic factors behind the partial resistance in Folke and identify molecular markers for use in marker-assisted selection. A population of 130 recombinant inbred lines was developed from a cross between Folke and the moderately susceptible spring wheat line T2038. The population was tested for powdery mildew resistance over two years at two locations in Norway and genotyped with DArT and SSR markers. Composite interval mapping detected a total of eight quantitative trait loci (QTL) for powdery mildew resistance; six with resistance from Folke on 2BS, 2DL, 5AL, 5BS and 6BS and two with resistance from T2038 on 5BS and 7AL. None of the loci with resistance from Folke mapped to chromosomal regions with known race-specific resistance genes, which confirmed the race non-specific nature of the resistance in this cultivar. The molecular markers linked to the reported QTL will be useful as a tool for selecting partial and potentially durable resistance to powdery mildew based on the resistance in Folke.
KeywordsWheat Triticum aestivum L. Powdery mildew Blumeria graminis f. sp. tritici Partial resistance Molecular markers
Funding for this research was received from the Research Council of Norway, Graminor Breeding Ltd, and the Norwegian Council of Genetic Resources. The authors are thankful to Yalew Tarkegne for technical assistance with the field trials and to Anne Guri Marøy for the molecular marker genotyping. We also thank Martin Ganal at TraitGenetics Inc., Gatersleben, Germany for providing unpublished primer sequences.
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