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Phenotypic evaluation of interspecific recombinant inbred lines (RILs) of Phaseolus species for aluminium resistance and shoot and root growth response to aluminium–toxic acid soil

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Abstract

Aluminium (Al) toxicity limits common bean productivity in acid soil regions of the tropics. To improve Al resistance of common bean, Al-sensitive Phaseolus vulgaris (SER16) was crossed to Al-resistant P. coccineus (G35346-3Q) to create 94 F5:6 recombinant inbred lines (RILs) of the pedigree SER16 × (SER16 × G35346-3Q). RILs were characterized for resistance to Al in a hydroponic system with 0 and 20 μM Al in solution, and for shoot and root growth response to Al-toxic infertile acid soil in 75 cm long soil cylinder system using an oxisol of low Al- (12.5%; pH 4.6; fertilized) and high Al-saturation (77%; pH 4.1; unfertilized). G35346-3Q increased its taproot elongation rate by 3.5% between 24 and 48 h under 20 μM Al in solution, while the best RIL, Andean genotype ICA Quimbaya, and sensitive genotype VAX1 expressed reductions of 2.6, 12.5, and 69.5%, respectively. In the acid soil treatment the correlation between leaf area and total root length was highly significant under high Al saturation (r = 0.70***). Genotypes that were Al resistant in the hydroponic system were not necessarily tolerant to Al-toxic acid soil conditions based on shoot and root growth responses. Phenotypic evaluation using both systems allows the identification of genotypes with Al resistance combined with acid soil adaptation. Two genotypes (ALB88 and ALB91) emerged as lines with multiple traits. Results suggest that inheritance of Al resistance and acid soil tolerance in G35346-3Q is complex. Results from this work will be useful for identification of molecular markers for Al resistance in Phaseolus species and to improve acid soil adaptation in common bean.

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Abbreviations

HAl:

High aluminium soil saturation

LA:

Leaf area

LAl:

Low aluminium soil saturation

MRD:

Mean root diameter

NRT:

Number of root tips

PC:

Percent of control

RDW:

Root dry weight

R:S:

Root to shoot ratio

SDW:

Shoot dry weight

SRL:

Specific root length

TRER24h:

Tap root elongation rate between 0–24 h

TRER24-48h:

Tap root elongation rate between 24–48 h

TRER48h:

Tap root elongation rate between 0–48 h

TRL:

Total root length

VRD34d:

Visual rooting depth at 34 days

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Acknowledgments

This research was supported by Bundesministerium für Wirtschaftliche Zusammenarbeit und Entwicklung (BMZ) project (No. 05.7860.9-001.00) entitled “Fighting drought and aluminum toxicity: Integrating functional genomics, phenotypic screening and participatory evaluation with women and small-scale farmers to develop stress-resistant common bean and Brachiaria for the tropics” granted to CIAT as a restricted core project. We are very grateful to research support staff of CIAT bean program for their assistance in data collection and processing. We thank Jaumer Ricaurte and Dr. Andres Felipe Rangel for their contribution to screening for resistance to aluminium in hydroponic system.

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Authors and Affiliations

  1. Centro Internacional de Agricultura Tropical (CIAT), A. A. 6713, Cali, Colombia

    Louis Butare, Idupulapati Rao, César Cajiao, José Polania, Juan Cuasquer & Stephen Beebe

  2. Unité de Phytopathologie, Gembloux Agro-Bio Tech, Université de Liège (ULg), Passage des Déportés, 2 B-5030, Gembloux, Belgium

    Louis Butare & Philippe Lepoivre

  3. Institut des Sciences Agronomiques du Rwanda (ISAR), 47 Rue Député Kamunzizi, Kigali, 5016, Rwanda

    Louis Butare

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  1. Louis Butare
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  2. Idupulapati Rao
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  4. César Cajiao
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  5. José Polania
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  7. Stephen Beebe
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Correspondence to Stephen Beebe.

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Butare, L., Rao, I., Lepoivre, P. et al. Phenotypic evaluation of interspecific recombinant inbred lines (RILs) of Phaseolus species for aluminium resistance and shoot and root growth response to aluminium–toxic acid soil. Euphytica 186, 715–730 (2012). https://doi.org/10.1007/s10681-011-0564-1

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