Skip to main content
SpringerLink
Log in

A QTL on chromosome 2DS of ‘Sumai 3’ increases susceptibility to Fusarium head blight in wheat

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Much effort has been invested in identifying molecular markers in wheat (Triticum aestivum L.) linked to quantitative trait loci (QTL) that confer resistance to Fusarium head blight (FHB) caused by Fusarium graminearum Schwabe [teleomorph Gibberella zeae (Schwein) Petch]. Even after several generations of crossing and selection by many wheat breeding programs, resistance of the Chinese spring wheat cultivar ‘Sumai 3’ (PI 481542) remains among the most effective. It therefore seems that undocumented resistance QTL present in Sumai 3 were not detected in various mapping studies. Using an extremely susceptible Tibetan landrace (‘Y1193-6’; unknown pedigree) in the creation of a mapping population with Sumai 3, the objective of this research was to identify undocumented resistance QTL in Sumai 3. This was accomplished through collecting disease index (DI) and Fusarium damaged kernel (FDK) phenotypic values along with 305 Diversity Array Technology (DArT) and 52 Simple Sequence Repeat (SSR) marker genotypes on 160 F2:6 recombinant inbred lines (RILs). Disease response evaluations were based on four (two greenhouse and two field) experiments where spray inoculation methods were used. Three QTL were identified on chromosome arms 3BS, 6BL and 2DS explaining 26.1, 10.7 and 18.9% of the phenotypic variation for DI, respectively. The same QTL were also significantly associated with reduced FDK scores and explained 28.0, 11.0 and 23.0% of phenotypic variation. Lines within the mapping population were placed in eight categories with respect to their various QTL combinations. Lines with no QTL were the most susceptible, whereas those with the Sumai 3-derived 3BS and 6BL QTL combined with the 2DS QTL from Y1193-6 were the most resistant. Though the 3BS and 6BL QTL are well-documented, the 2DS resistance QTL, which was contributed by the susceptible parent, confers increased susceptibility when derived from Sumai 3. In this study no new FHB QTL from Sumai 3 was discovered, but results suggest that Sumai 3 contains a QTL for susceptibility on chromosome arm 2DS. Selection against this QTL may potentially increase resistance levels among Sumai 3-derived populations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Akbari M, Wenzl P, Caig V, Carling J, Xia L, Yang S, Uszynski G, Mohler V, Lehmesiek A, Kuchel H, Hayden MJ, Howes N, Sharp P, Vaughan P, Rathmell B, Huttner E, Kilian A (2006) Diversity arrays technology (DArT) for high-throughput profiling of the hexaploid wheat genome. Theor Appl Genet 113:1409–1420

    Article  PubMed  CAS  Google Scholar 

  • Anderson JA, Waldron BL, Moreno-Sevilla B, Stack RW, Frohberg RC (1998) DNA markers for Fusarium head blight resistance QTL in two wheat population. In: Hart P, Ward R, Bafus R, Bedford K (eds) Proc 1998 National FHB Forum. University Printing Office, University of Michigan, East Lansing, MI, pp 57–59

  • Anderson JA, Stack RW, Liu S, Waldron BL, Field AD, Coyne C, Moreno-Sevilla B, Fetch JM, Song QJ, Cregan PB, Frohberg RC (2001) DNA markers for Fusarium head blight resistance QTL in two wheat populations. Theor Appl Genet 102:1164–1168

    Article  CAS  Google Scholar 

  • Bai G, Shaner G (1994) Scab of wheat: prospects for control. Plant Dis 78:760–766

    Article  Google Scholar 

  • Bai G, Shaner G (2004) Management and resistance in wheat and barley to Fusarium head blight. Annu Rev Phytopathol 42:135–161

    Article  PubMed  CAS  Google Scholar 

  • Buerstmayr H, Lemmens M, Hart L, Doldi L, Steiner B, Stierschneider M, Ruckenbauer P (2002) Molecular mapping of QTL for Fusarium head blight resistance in spring wheat I. Resistance to fungal spread (type II) resistance. Theor Appl Genet 104:84–91

    Article  PubMed  CAS  Google Scholar 

  • Buerstmayr H, Steiner B, Hartl L, Griesser M, Angerer N, Lengauer D, Miedaner T, Schneide B, Lemmens M (2003) Molecular mapping of QTL for Fusarium head blight resistance in spring wheat II. Resistance to fungal penetration and spread. Theor Appl Genet 107:503–508

    Article  PubMed  CAS  Google Scholar 

  • Buerstmayr H, Ban T, Anderson JA (2009) QTL mapping and marker-assisted selection for Fusarium head blight resistance in wheat: a review. Plant Breed 128:1–26

    Article  CAS  Google Scholar 

  • Draeger R, Gosman N, Steed A, Chandler E, Thomsett M, Srinivasachary S, Schondelmaier J, Buerstmayr H, Lemmens M, Schmolke M, Mesterhazy A, Nicholson P (2007) Identification of QTL for resistance to Fusarium head blight, DON accumulation and associated traits in the winter wheat variety Arina. Theor Appl Genet 15:617–625

    Article  Google Scholar 

  • Gupta PK, Balyan HS, Edwards KJ, Isaac P, Korzun V, Roder M, Gautier MF, Joudrier P, Schlatter AR, Dubcovsky J, De la Pena RC, Khairallah M, Penner G, Hayden MJ, Sharp P, Keller B, Wang RCC, Hardouin JP, Jack P, Leroy P (2002) Genetic mapping of 66 new microsatellite (SSR) loci in bread wheat. Theor Appl Genet 105:413–422

    Article  PubMed  CAS  Google Scholar 

  • Handa H, Namiki N, Xu D, Ban T (2008) Dissecting of the FHB resistance QTL on the short arm of wheat chromosome 2D using a comparative genomic approach: from QTL to candidate gene. Mol Breed 22:71–84

    Article  Google Scholar 

  • Holzapfel J, Voss HH, Miedaner T, Korzun V, Häberle J, Schweizer G, Mohler V, Zimmermann G, Hartl L (2008) Inheritance of resistance to Fusarium head blight in three European winter wheat populations. Theor Appl Genet 117:1119–1128

    Article  PubMed  Google Scholar 

  • Jaccoud D, Peng K, Feinstein D, Kilian A (2001) Diversity arrays: a solid state technology for sequence information independent genotyping. Nucleic Acids Res 29:e25

    Article  PubMed  CAS  Google Scholar 

  • Jia G, Chen P, Qin G, Bai G, Wang X, Wang S, Zhou B, Zhang S, Liu D (2005) QTL for Fusarium head blight response in a wheat DH population of Wangshuibai/Alondra‘s’. Euphytica 146:183–191

    Article  CAS  Google Scholar 

  • Jones RK, Mirocha CJ (1999) Quality parameters in small grains from Minnesota affected by Fusarium head blight. Plant Dis 83:506–511

    Article  Google Scholar 

  • Kadariya M, Glover KD, Mergoum M, Osborne LE (2008) Biplot analysis of agronomic and Fusarium head blight resistance traits in spring wheat. J Crop Improvement 22:147–170

    Article  Google Scholar 

  • Knapp SJ, Stroup WW, Ross WM (1985) Exact confidence intervals for heritability on a progeny mean basis. Crop Sci 25:192–194

    Article  Google Scholar 

  • Liu ZZ, Wang ZY (1990) Improved scab resistance in China: sources of resistance and problems. In: Saunders DA (ed) Wheat for the nontraditional warm areas, Proceedings of the international conferences, Foz do Iguacu, Brazil. International Maize and Wheat Improvement Center (CIMMYT), Mexico, pp 178–187

  • Malla S, Ibrahim AMH, Yen Y, Berzonsky W, Glover KD, Stein J (2010) QTL analysis of a putative novel source of resistance to Fusarium head blight in hard winter wheat. Int J Plant Breed 4:47–54

    Google Scholar 

  • Manly KF, Cudmore RH Jr, Meer JM (2001) MapManager QTX: cross-platform software for genetic mapping. Mamm Genome 12:930–932

    Article  PubMed  CAS  Google Scholar 

  • McMullen M, Jones R, Gallenberg D (1997) Scab of wheat and barley: a re-emerging disease of devastating impact. Plant Dis 81:1340–1348

    Article  Google Scholar 

  • Mesterhazy A (1995) Types and components of resistance to Fusarium head blight of wheat. Plant Breed 114:377–386

    Article  Google Scholar 

  • Miedaner T (1997) Review: breeding wheat and rye for resistance to Fusarium diseases. Plant Breed 116:201–220

    Article  Google Scholar 

  • Pestsova E, Ganal MW, Roder MS (2000) Isolation and mapping of microsatellite markers specific for the D genome of bread. Wheat Genome 43:689–697

    Article  CAS  Google Scholar 

  • Ren LJ, Shen XR, Zhou MP, Zhang X, Ma HX, Lu WZ, Nichoson P (2003) SSR markers for Fusarium head blight resistance QTL in three wheat populations. Agric Sci China 2:987–993

    Google Scholar 

  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023

    PubMed  Google Scholar 

  • Rosyara UR, Gonzalez-Hernandez JL, Glover KD, Gedye KR, Stein JM (2009) Family-based mapping of quantitative trait loci in plant breeding populations with resistance to Fusarium head blight in wheat as an illustration. Theor Appl Genet 118:1617–1631

    Article  PubMed  CAS  Google Scholar 

  • Rudd JC, Horsley RD, McKendry AL, Elias EM (2001) Host plant resistance genes for Fusarium head blight: sources, mechanisms, and utility in conventional breeding systems. Crop Sci 41:620–627

    Article  Google Scholar 

  • Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer length polymorphism in barley: mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81:8014–8018

    Article  PubMed  CAS  Google Scholar 

  • Schroeder HW, Christensen JJ (1963) Factors affecting resistance of wheat to scab caused by Gibberella zeae. Phytopathology 53:831–838

    Google Scholar 

  • Shen X, Zhou M, Lu W, Ohm H (2003) Detection of Fusarium head blight QTL in a wheat population using bulk segregant analysis. Theor Appl Genet 106:1041–1047

    PubMed  CAS  Google Scholar 

  • Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114. doi:10.1007/s00122-004-1740-7

    Article  PubMed  CAS  Google Scholar 

  • Song QJ, Fickus EW, Cregan PB (2002) Characterization of trinucleotide SSR motifs in wheat. Theor Appl Genet 104:286–293

    Article  PubMed  CAS  Google Scholar 

  • Sourdille P, Tavaud M, Charmet G, Bernard M (2001) Transferability of wheat microsatellite to diploid Triticeae species carrying the A, B and D genomes. Theor Appl Genet 103:346–352

    Article  CAS  Google Scholar 

  • Stack RW, McMullen MP (1998) A visual scale to estimate severity of Fusarium head blight in wheat. Circ, p 1095. North Dakota State Univ Ext Serv, Fargo, ND. Available at www.ext.nodak.edu/extpubs/plantsci/smgrains/pp1095w.htm. Accessed 15 May 2011

  • Van Os H, Stam P, Visser RGF, Van Eck HJ (2005) RECORD: a novel method for ordering loci on a genetic linkage map. Theor Appl Genet 112:30–40

    Article  PubMed  CAS  Google Scholar 

  • Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTL. J Hered 93:77–78

    Article  PubMed  CAS  Google Scholar 

  • Waldron BL, Moreno-Sevilla B, Anderson JA, Stack RW, Frohberg RC (1999) RFLP mapping of QTL for Fusarium head blight resistance in wheat. Crop Sci 39:805–811

    Article  CAS  Google Scholar 

  • Wenzl P, Carling J, Kudrna D, Jaccoud D, Huttner E, Kleinhofs A, Kilian A (2004) Diversity Arrays Technology (DArT) for whole-genome profiling of barley. Proc Natl Acad Sci USA 101:9915–9920

    Article  PubMed  CAS  Google Scholar 

  • Windels CE (2000) Economic and social impacts of Fusarium head blight: changing farms and rural communities in the Northern Great Plains. Phytopathology 90:17–21

    Article  PubMed  CAS  Google Scholar 

  • Xie Y, McNally K, Li C, Leung H, Zho Y (2006) A high-throughput genomic tool: Diversity Array Technology complementary for rice genotyping. J Int Plant Biol 48:1069–1076

    Article  CAS  Google Scholar 

  • Xu DH, Juan HF, Nohda M, Ban T (2001) QTL mapping of Type I and Type II resistance to FHB in wheat. In: Proceedings national Fusarium head blight forum, Cincinnati, 8–10 December, 2001, pp 40–42

  • Yang J, Zhu J, Williams RW (2007) Mapping the genetic architecture of complex traits in experimental populations. Bioinformatics 23:1527–1536

    Article  PubMed  CAS  Google Scholar 

  • Yao JB, Ge YF, Wang SW (1997) Chromosomal location of genes for scab resistance in wheat cultivar Sumai 3. Acta Agron Sin 23:450–453

    Google Scholar 

  • Yu YJ (1982) Monosomic analysis for scab resistance and yield components in the wheat cultivar Soo-mo. Cereal Res Commun 10:185–189

    Google Scholar 

  • Yu J (2007) Identification of new sources and mapping of QTL for FHB resistance in Asian wheat germplasm. PhD dissertation, Kansas State University, Manhattan, KS

  • Zhang M, Zhang R, Yang J, Leo P (2010) Identification of a new QTL for Fusarium head blight resistance in the wheat genotype “Wang shui-bai”. Mol Biol Rep 37:1031–1035

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Soil and Crop Sciences, Texas A&M University, College Station, TX, 77843, USA

    Bhoja R. Basnet & Amir M. H. Ibrahim

  2. Department of Plant Sciences, South Dakota State University, Brookings, SD, 57007, USA

    Karl D. Glover

  3. Department of Biology and Microbiology, South Dakota State University, Brookings, SD, 57007, USA

    Yang Yen

  4. USDA-ARS Bioscience Research Laboratory, Fargo, ND, 58102, USA

    Shiaoman Chao

Authors
  1. Bhoja R. Basnet
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Karl D. Glover
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Amir M. H. Ibrahim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yang Yen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Shiaoman Chao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Bhoja R. Basnet.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary Table 1

List of markers, linkage groups and corresponding map distances (XLS 77 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Basnet, B.R., Glover, K.D., Ibrahim, A.M.H. et al. A QTL on chromosome 2DS of ‘Sumai 3’ increases susceptibility to Fusarium head blight in wheat. Euphytica 186, 91–101 (2012). https://doi.org/10.1007/s10681-011-0495-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-011-0495-x

Keywords

Search

Navigation