, 181:429 | Cite as

QTL mapping for chromium-induced growth and zinc, and chromium distribution in seedlings of a rice DH population

  • Boyin Qiu
  • Fanrong Zeng
  • Dawei Xue
  • Weihui Zhou
  • Shafaqat Ali
  • Guoping Zhang


Chromium contamination in soil has become a severe threat to crop production and food safety. The experiment was conducted using a rice DH population to detect the QTLs associated with Cr tolerance. Seventeen putative QTLs associated with growth traits included three additive loci and fourteen epistatic loci. These loci were distributed on 11 rice chromosomes, and their contribution to the phenotypic variation ranged from 2.44 to 10.08%. Two QTLs located at the similar genetic region on chromosome ten were associated with shoot Cr concentration and translocation from roots to shoots, respectively; and they accounted for 11.65 and 11.22% of the phenotypic variation. In addition, six QTLs related to Zn concentration and translocation was found on chromosomes 1, 2, 4, 5, 7 and 12. Meanwhile epistatic effect existed in the two additive QTLs of qRZC1 and qRZC7. Most of QTLs controlling Zn concentration had small genotypic variance and qSRZ4 related to Zn translocation showed growth condition-dependent expression.


Chromium Zinc Chromosome Double haploid population QTL analysis Rice (Oryza sativa L.) 









Quantitative trait loci


Chlorophyll content


Root length


Plant height


Shoot dry weight


Root dry weight


Shoot Cr concentration


Root Cr concentration


Shoot Zn concentration


Root Zn concentration


Ratio of shoot Cr concentration to root Cr concentration


Ratio of shoot Zn concentration to root Zn concentration


Reduction of SRZ in control to Cr treatment



The project was supported by Zhejiang Bureau of Science and Technology (2009C12050) and Wenzhou Bureau of Science and Technology. We are indebted to Mr. Zhihong Zhu for his help in QTL analysis.


  1. Becquer T, Quantin C, Sicot M, Boudot JP (2003) Chromium availability in ultramafic soils from New Caledonia. Sci Total Environ 301:251–261PubMedCrossRefGoogle Scholar
  2. Chen YX, Zhu ZX, He ZY (1994) Mechanisms of chromium transformations in soils and its effects on rice growth. J Zhejiang Agric Univ 20(1):1–7Google Scholar
  3. Costa M (2000) Chromium and nickel. In: Zalups RK, Koropatnick J (eds) Molecular biology and toxicology of metals. Taylor and Francis, Great Britain, pp 113–114Google Scholar
  4. Dasgupta T, Hossain SA, Meharg AA, Price AH (2004) An arsenate tolerance gene on chromosome 6 of rice. New Phytol 163:45–49CrossRefGoogle Scholar
  5. Davies FT, Puryear JD, Newton RJ, Egilla JN, Grossi JAS (2002) Mycorrhizal fungi increase chromium uptake by sunflower plants: influence on tissue mineral concentration, growth, and gas exchange. J Plant Nutr 25:2389–2407CrossRefGoogle Scholar
  6. Dong Y, Ogawa T, Lin D, Koh H, Kamiunten H, Matsuo M, Cheng S (2006) Molecular mapping of quantitative trait loci for zinc toxicity tolerance in rice seedling (Oryza sativa L.). Field Crops Res 95:420–425CrossRefGoogle Scholar
  7. Järup L, Berglund M, Elinder CG, Nordberg G, Vahter M (1998) Health effects of cadmium exposure-a review of the literature and a risk estimate. Scand J Work Environ Health 24:1–51PubMedGoogle Scholar
  8. Lu C, Shen L, Tan Z, Xu Y, He P, Chen Y, Zhu L (1996) Comparative mapping of QTLs for agronomic traits of rice across environments using a doubled haploid population. Theor Appl Genet 93:1211–1217CrossRefGoogle Scholar
  9. Ma JF, Shen R, Zhao Z, Wissuwa M, Takeuchi Y, Ebitani T, Yano M (2002) Response of rice to Al stress and identification of quantitative trait loci for Al tolerance. Plant Cell Physiol 43:652–659PubMedCrossRefGoogle Scholar
  10. Mao CZ, Yang L, Zheng BS WUYR, Liu FY, Yi KK, Wu P (2004) Comparative mapping of QTLs for Al tolerance in rice and identification of positional Al-induced genes. J Zhejiang Univ Sci 5(6):634–643PubMedCrossRefGoogle Scholar
  11. McCouch SR, Cho YG, Yano M, Paul E, Blinstrub M (1997) Report on QTL nomenclature. Rice Genet Newsl 14:11–13Google Scholar
  12. McLaughlin MJ, Parkerb DR, Clarke JM (1999) Metals and micronutrients-food safety issues. Field Care 60:143–163CrossRefGoogle Scholar
  13. Mishra S, Shanker K, Srivastava MM, Srivastava S, Shrivastav R, Dass S et al (1997) A study on the uptake of trivalent and hexavalent chromium by paddy (Oryza sativa): possible chemical modifications in rhizosphere. Agric Ecosyst Environ 62:53–58CrossRefGoogle Scholar
  14. Narasimhamoorthy B, Bouton HJ, Olsen MK, Sledge KM (2007) Quantitative trait loci and candidate gene mapping of aluminum tolerance in diploid alfalfa. Theor Appl Genet 114:901–913PubMedCrossRefGoogle Scholar
  15. Nguyen VT, Burow MD, Nguyen HT, Le BT, Le TD, Paterson AH (2001) Molecular mapping of genes conferring aluminum tolerance in rice (Oryza sativa L.). Theor Appl Genet 102:1002–1010CrossRefGoogle Scholar
  16. Norton JG, Aitkenhead JM, Khowaja SF, Whalley RW, Price HA (2008) A bioinformatic and transcriptomic approach to identifying positional candidate genes without fine mapping: an example using rice root-growth QTLs. Genomics 92:344–352PubMedCrossRefGoogle Scholar
  17. Nriagu JO (1988) Production and uses of chromium, chromium in natural and human environment. Wiley, New York, pp 81–105 USA7Google Scholar
  18. Qiu BY, Zhou WH, Xue DW, Zeng FR, Ali S, Zhang GP (2010) Identification of Cr-tolerant lines in a rice (Oryza sativa) DH population. Euphytica 174:199–207CrossRefGoogle Scholar
  19. Shahandeh H, Hossner LR (2000) Plant screening for chromium phytoremediation. Int J Phytoremed 2(1):31–51CrossRefGoogle Scholar
  20. Teng S, Qian Q, Zeng DL, Kunihiro Y, Fujimoto K, Huang DN, Zhu LH (2004) QTL analysis of leaf photosynthetic rate and related physiological traits in rice (Oryza sativa L.). Euphytica 135:1–7CrossRefGoogle Scholar
  21. Ueno D, Emi Koyama E, Kono I, Ando T, Yano M, Ma JF (2009) Identification of a novel major quantitative trait locus controlling distribution of Cd between roots and shoots in rice. Plant Cell Physiol 50:2223–2233PubMedCrossRefGoogle Scholar
  22. Wallace A, Soufi SM, Cha JW, Romney EM (1976) Some effects of chromium toxicity on bush bean plants grown in soil. Plant Soil 44:471–473CrossRefGoogle Scholar
  23. Wan J, Zhai H, Wan J, Ikehashi H (2003) Detection and analysis of QTLs ferrous iron toxicity tolerance in rice. Euphytica 131:201–206CrossRefGoogle Scholar
  24. Wang DL, Zhu J, Li ZK, Paterson AH (1999) Mapping QTLs with epistatic effects and QTL × environment interactions by mixed linear model approaches. Theor Appl Genet 99:1255–1264CrossRefGoogle Scholar
  25. Wang XY, Wu P, Wu YR, Yan XL (2002) Molecular marker analysis of manganese toxicity tolerance in rice under green house conditions. Plant Soil 238:227–233CrossRefGoogle Scholar
  26. Wissuwa M, Ismail MA, Yanagihara S (2006) Effects of zinc deficiency on rice growth and genetic factors contributing to tolerance. Plant Physiol 142:731–741PubMedCrossRefGoogle Scholar
  27. Wu P, Luo A, Zhu J, Yang J, Huang N, Senadhira D (1997) Molecular markers linked to genes underlying seedling tolerance for ferrous iron toxicity. Plant Soil 196:317–320CrossRefGoogle Scholar
  28. Xu YB, Shen LS, McCouch SR, Zhu LH (1998) Extension of the rice DH population genetic map with microsatellite markers. Chin Sci Bull 42:149–152Google Scholar
  29. Xue Y, Wan JM, Jiang L, Liu LL, Su N, Zhai HQ, Ma JF (2006) QTL analysis of aluminum resistance in rice (Oryza sativa L.). Plant Soil 287:375–383CrossRefGoogle Scholar
  30. Xue DW, Chen MC, Zhang GP (2009) Mapping of QTLs associated with cadmium tolerance and accumulation during seedling stage in rice (Oryza sativa L.). Euphytica 165:587–596CrossRefGoogle Scholar
  31. Yamamoto T, Yonemaru J, Yano M (2009) Towards the understanding of complex traits in rice: substantially or superficially? DNA Res 16:141–154PubMedCrossRefGoogle Scholar
  32. Yang J, Zhu J (2005) Predicting superior genotypes in multiple environments based on QTL effects. Theor Appl Genet 110:1268–1274PubMedCrossRefGoogle Scholar
  33. Yang QH, Lu W, Hu ML, Wang CM, Bang RX, Yano M, Wan JM (2003) QTL and epistatic interaction underlying leaf chlorophyll and H2O2 content variation in rice. Acta Genet Sin 30:245–250 (in Chinese)PubMedGoogle Scholar
  34. Yoshida S, Forna DA, Cock JH, Gomez KA (1976) Laboratory manual for physiological studies of rice. International Rice Research Institute, Los Banos, pp 62–63Google Scholar
  35. Zeng FR, Mao Y, Cheng WD, Wu FB, Zhang GP (2008) Genotypic and environmental variation in chromium, cadmium and lead concentrations in rice. Environ Pollut 153(2):309–314PubMedCrossRefGoogle Scholar
  36. Zhang J, Zhu YG, Zeng DL, Cheng WD, Qian Q, Duan GL (2008) Mapping quantitative trait loci associated with arsenic accumulation in rice (Oryza sativa). New Phytol 177:350–356PubMedGoogle Scholar
  37. Zhu LH, Chen Y, Xu YB, Xu JC, Cai HW, Ling ZZ (1993) Construction of a molecular map of rice and gene mapping using a double haploid population of a cross between Indica and Japonica varieties. Rice Genet Newsl 10:132–134Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Boyin Qiu
    • 1
  • Fanrong Zeng
    • 1
  • Dawei Xue
    • 2
  • Weihui Zhou
    • 1
  • Shafaqat Ali
    • 1
  • Guoping Zhang
    • 1
  1. 1.Agronomy DepartmentZhejiang UniversityHangzhouChina
  2. 2.College of Life and Environment Sciences, Hangzhou Normal UniversityHangzhouChina

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