Skip to main content
SpringerLink
Log in

A novel blast resistance locus in a rice (Oryza sativa L.) cultivar, Chumroo, of Bhutan

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

The rice cultivar ‘Chumroo’ is commonly cultivated in the mid- and high-altitude areas of Bhutan. This cultivar has shown durable blast resistance in that area, without evidence of breakdown, for over 20 years. Chumroo was inoculated with 22 blast isolates selected from the race differential standard set of Japan. The cultivar showed resistance to all the isolates. To identify the resistance gene(s), Chumroo was crossed with a susceptible rice cultivar, Koshihikari. The F1 plants of the cross showed resistance. Segregation analyses of 300 F3 family lines fitted the segregation ratio of 1:2:1 and indicated that a single dominant gene controls the resistance to a blast isolate Ao 92-06-2 (race 337.1). The Chumroo resistance locus (termed Pi46(t)) was mapped between two SSR markers, RM6748 and RM5473, on the terminal region of the long arm of chromosome 4, using linkage analysis with SSR markers. The nearest marker, RM5473, was linked to the putative resistance locus at a map distance of 3.2 cM. At the chromosomal region, no true resistance genes were identified, whereas two field resistance genes were present. Therefore, we designated Pi46(t) as a novel blast resistance locus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Abe S (2004) Breeding of a blast resistant multiline variety of rice, Sasanishiki BL. JARQ 38:149–154

    Google Scholar 

  • Asaga K (1981) A procedure for evaluating field resistance to blast in rice varieties. J Cent Agric Exp Stn 35:51–138 (in Japanese with English summary)

    Google Scholar 

  • Bonman JM (1992) Durable resistance to rice blast disease—environmental influences. Euphytica 63:115–123

    Article  Google Scholar 

  • Borlaug NE (1953) New approach to the breeding of wheat varieties resistant to Puccinia graminis tritici. Phytopathology 43:467

    Google Scholar 

  • Chen DH, dela Vina M, Inukai T, Mackill DJ, Ronald PC, Nelson RJ (1999) Molecular mapping of the blast resistance gene, Pi44(t), in a line derived from a durably resistant rice cultivar. Theor Appl Genet 98:1046–1053

    Article  CAS  Google Scholar 

  • Couch BC, Kohn LM (2002) A multilocus gene genealogy concordant with host preference indicates segregation of a new species, Magnaporthe oryzae, from M. grisea. Mycologia 94:683–693

    Article  PubMed  CAS  Google Scholar 

  • Ezuka A (1972) Field resistance of rice varieties to blast disease. Rev Plant Prot Res 5:1–21

    Google Scholar 

  • Flor HH (1956) The complementary genetic systems in flax and flax rust. Adv Genet 8:29–54

    Article  Google Scholar 

  • Fukuta Y, Xu D, Kobayashi N, Telebanco-Yanoria MJ, Hairmansis A, Hayashi N (2009) Genetic characterization of universal differential varieties for blast resistance developed under the IRRI-Japan Collaborative Research Project using DNA markers in rice (Oryza sativa L.). JIRCAS Working Report No 63, pp 35–68

  • Hayashi N (2005) MAFF microorganism genetic resources manual 18: rice blast fungus. National Institute of Agrobiological Sciences, Tsukuba

    Google Scholar 

  • Hayashi N, Fukuta Y (2009) Proposal for a new international system of differentiating races of blast (Pyricularia oryzae Cavara) by using LTH monogenic lines in rice (Oryza sativa L.). JIRCAS Working Report No 63, pp 11–16

  • Hayashi N, Kobayashi N, Vera Cruz CM, Fukuta Y (2009) Protocols for the sampling of diseased specimens and evaluation of blast disease in rice. JIRCAS Working Report No 63, pp 17–33

  • Ise K (1996) Statistical analysis. In: Yamamoto R, Horisue N, Ikeda R (eds) Rice breeding manual. Yokendo, Tokyo, pp 244–251 (in Japanese)

    Google Scholar 

  • Ishizaki K (2007) Studies on practical application of multiline cultivar, Koshihikari, with blast resistance genes in Niigata Prefecture. J Niigata Agric Res Inst 8:1–37 (in Japanese with English summary)

    Google Scholar 

  • Jensen NF (1952) Intra-varietal diversification in oat breeding. Agron J 44:30–34

    Article  Google Scholar 

  • Kinoshita T, Rothschild G (1995) A report of the coordinating committee of rice genetics cooperative. Rice Genet Newsl 12:5–6

    Google Scholar 

  • Kiyosawa S (1982) Genetics and epidemiological modeling of breakdown of plant disease resistance. Ann Rev Phytopathol 20:93–117

    Article  Google Scholar 

  • Kiyosawa S (1984) Establishment of differential varieties for pathogenicity test of rice blast. Rice Genet Newsl 1:95–97

    Google Scholar 

  • Kobayashi N, Terebanco-Yanoria MJ, Tsunematsu H, Kato H, Imbe T, Fukuta Y (2007) Development of new sets of international standard differential varieties for blast resistance in rice (Oryza sativa L.). JARQ 41:31–37

    Google Scholar 

  • Kobayashi N, Ebron LA, Fujita D, Fukuta Y (2009) Identification of blast resistance genes in IRRI-bred rice varieties by segregation analysis based on a differential system. JIRCAS Working Report No 63, pp 69–86

  • Koizumi S, Iwano M, Zenbayashi K, dela Peña FA, Sonoda R, Nakajima T, Arai M, Nakajima T, Miyasaka A, Ashizawa T, Yasuda N, Noguchi M (2007) Distribution of pathogenic races of the rice blast fungus in Japan in 2001. Misc Publ Natl Agric Res Cent 7:1–63 (in Japanese)

    Google Scholar 

  • Kojima Y, Ebitani T, Kaneda H, Doi M, Ishibashi T, Kidani Y, Mukaino N, Yamaguchi T, Omoteno M, Yamamoto Y (2003) Development and utilization of isogenic lines Koshihikari Toyama II. Development of isogenic lines Koshihikari Toyama BL. Bull Toyama Agric Res Cent 20:13–32 (in Japanese with English summary)

    Google Scholar 

  • Lander ES, Green O, Abrahanson J (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  PubMed  CAS  Google Scholar 

  • McCouch SR, Teytelman L, Xu Y, Lobos KB, Clare K, Walton M, Fu B, Maghirang R, Li Z, Xing Y, Zhang Q, Kono I, Yano M, Fjellstrom R, DeClerck G, Schneider D, Cartinhour S, Ware D, Stein L (2002) Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.). DNA Res 9:199–207

    Article  PubMed  CAS  Google Scholar 

  • Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325

    Article  PubMed  CAS  Google Scholar 

  • Noda T, Hayashi N, Du PV, Dinh HD, E LV (1999) Distribution of pathogenic races of rice blast fungus in Vietnam. Ann Phytopathol Soc Jpn 65:526–530

    Google Scholar 

  • Panaud O, Chen X, McCouch SR (1996) Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.). Mol Gen Genet 252:597–607

    PubMed  CAS  Google Scholar 

  • Parlevliet JE (1979) Components of resistance that reduce the rate of epidemic development. Ann Rev Phytopathol 17:203–222

    Article  Google Scholar 

  • Silué D, Notteghem JL, Tharreau D (1992) Evidence of a gene-for-gene relationship in the Oryza sativa–Magnaporthe grisea pathosystem. Phytopathology 82:577–580

    Article  Google Scholar 

  • Telebanco-Yanoria MJ, Imbe T, Kato H, Tsunematsu H, Ebron LA, Vera Cruz CM, Kobayashi N, Fukuta Y (2008) A set of standard differential blast isolates (Magnaporthe grisea (Herbert) Barr.) from the philippines for rice (Oryza sativa L.) resistance. JARQ 42:23–34

    Google Scholar 

  • Temnykh S, Park WD, Ayres N, Cartinhour S, Hauck N, Lipovich L, Cho TG, Ishii T, McCouch SR (2000) Mapping and genome organization of microsatellite sequences in rice (Oryza sativa L.). Theor Appl Genet 100:697–712

    Article  CAS  Google Scholar 

  • Terashima T, Fukuoka S, Saka N, Kudo S (2008) Mapping of a blast field resistance gene Pi39(t) of elite rice strain Chubu 111. Plant Breed 127:485–489

    Article  Google Scholar 

  • Thinlay, Koizumi S, Ashizawa T, Zenbayashi K (2000a) Characterization of resistance to blast in Bhutanese rice cultivars. Jpn J Phytopathol 66:267

    Google Scholar 

  • Thinlay, Zeigler RS, Finckh MR (2000b) Pathogenic variability of Pyricularia grisea from the high- and mid-elevation zone of Bhutan. Phytopathology 90:621–628

    Article  PubMed  CAS  Google Scholar 

  • Xu X, Chen H, Fujimura T, Kawasaki S (2008) Fine mapping of a strong QTL of field resistance against rice blast, Pikahei-1(t), from upland rice Kahei, utilizing a novel resistance evaluation system in the greenhouse. Theor Appl Genet 117:997–1008

    Article  PubMed  Google Scholar 

  • Yamada M, Kiyosawa S, Yamaguchi T, Hirano T, Kobayashi T, Kushibuchi K, Watanabe S (1976) Proposal of a new method for differentiating races of Pyricularia oryzae Cavara in Japan. Ann Phytopathol Soc Jpn 42:216–219

    Google Scholar 

  • Yasuda N, Fujita Y, Noguchi M (2004) Identification of avirulence genes in the rice blast fungus corresponding to three resistance genes in Japanese differentials. J Gen Plant Pathol 70:202–206

    Article  CAS  Google Scholar 

  • Yasuda N, Noguchi MT, Fujita Y (2005) Identification of an Avirulence gene in Magnaporthe grisea corresponding to a resistance gene at Pik locus. Phytopathology 95:768–772

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We thank Dr. T. Nagamine and Dr. T. Ishii, National Agricultural Research Center for Western Region (WeNARC), Dr. M. Kawase, National Institute of Agrobiological Sciences, and Dr. K. Matsuya, Japan International Cooperation Agency for their technical advice. We also thank Dr. E. Araki, WeNARC, for providing the SSR markers. We also thank the field technicians of the Technical Support Section, WeNARC, who provided the plant materials. We also thank Ms. N. Kobayashi, Ms. I. Ikeda, Ms. K. Hanada, Ms. Y. Tashima, and Ms. M. Oda for their technical assistance.

Author information

Authors and Affiliations

  1. National Agricultural Research Center for Western Region, 6-12-1 Nishi-Fukatsu, Fukuyama, Hiroshima, 721-8514, Japan

    Kei Matsushita, Shuichi Iida & Osamu Ideta

  2. National Agricultural Research Center, 3-1-1 Kannondai, Tsukuba, Ibaraki, 305-8666, Japan

    Nobuko Yasuda, Shinzo Koizumi & Yoshikatsu Fujita

  3. National Plant Protection Center, P.O. Box 670, Simtokha, Thimphu, Bhutan

    Thinlay

  4. National Agricultural Research Center for Tohoku Region, 3 Shimo-furumichi, Yotsuya, Daisen, Akita, 014-0102, Japan

    Taketo Ashizawa

  5. National Institute of Crop Science, 2-1-18 Kannondai, Tsukuba, Ibaraki, 305-8518, Japan

    Yoshihiro Sunohara & Hideo Maeda

Authors
  1. Kei Matsushita
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nobuko Yasuda
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Thinlay
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shinzo Koizumi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Taketo Ashizawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yoshihiro Sunohara
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Shuichi Iida
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Osamu Ideta
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Hideo Maeda
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Yoshikatsu Fujita
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kei Matsushita.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Matsushita, K., Yasuda, N., Thinlay et al. A novel blast resistance locus in a rice (Oryza sativa L.) cultivar, Chumroo, of Bhutan. Euphytica 180, 273–280 (2011). https://doi.org/10.1007/s10681-011-0405-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-011-0405-2

Keywords

Search

Navigation