Advertisement

Euphytica

, Volume 179, Issue 3, pp 417–425 | Cite as

Determination of responses of different bean cultivars against races of Pseudomonas syringae pv phaseolicola, causal agent of halo blight of bean

  • İmam Adem Bozkurt
  • Soner Soylu
Article

Abstract

Use of resistant plant varieties combined with other disease management practices is regarded as the most practical approach to control of seed-borne bacterial disease agents. In this study, responses of different bean cultivars to nine different races of Pseudomonas syringae pv phaseolicola, the causal agent of bacterial halo blight of common bean (Phaseolus vulgaris L.), were determined. During compatible interaction in susceptible cultivars, virulent bacterial races caused water soaked lesion at sites of inoculation. Similar lesions developed in moderately resistant cultivars but symptoms were later associated with more tissue browning around the sites of inoculation. In contrast, the resistant response, produced the characteristic hypersensitive reaction (HR), was characterized as a small discrete browning and tissue collapse at site of inoculation. No local cultivars showed complete resistance to all races tested. Bean cultivars Sehirali-90 and Göynük-98 were found to be resistant or moderately resistant to five different bacterial races. Bean cultivar, Karacaşehir-90, on the other hand, was found to be resistant or moderately resistant to six different bacterial races. Analysis of bacterial growth and the accumulation of isoflavonoid bean phytoalexin, phaseollin in planta were carried out for tissues expressing compatible and incompatible interactions to enable a link to be made between reaction phenotypes and restriction of bacterial growth and phytoalexin accumulations. Development of the HR was clearly associated with the restricted multiplication of bacteria during incompatible interactions. A time-course accumulation analysis on pods treated with different races of bacterial agent showed that a strong correlation was observed between the timing and extent of cell death and accumulation of phaseollin, being rapid and extensive in incompatible interactions compared to compatible interaction.

Keywords

Bean Halo blight Phaseollin Phytoalexin Resistance 

Notes

Acknowledgments

The authors wish to thank Prof. Dr. John W. Mansfield (Department of Biology, Imperial College, and UK) for providing bacterial strains and M.H. Bennett, for technical assistance and helpful discussions for the isolation and quantification of phaseollin. The work was supported by a grant from the Mustafa Kemal University Scientific Research Fund.

References

  1. Allen DJ, Buruchara RA, Smithson JB (1998) Diseases of common bean. In: Allen DJ, Lenne JM (eds) The pathology of food and pasture legumes. CAB International, Wallingford, pp 179–235Google Scholar
  2. Bailey JA, Burden RS (1973) Biochemical changes and phytoalexin accumulation in Phaseolus vulgaris following cellular browning caused by Tobacco Necrosis Virus. Physiol Plant Pathol 3:171–177CrossRefGoogle Scholar
  3. Bailey J, Rowell P, Arnold G (1980) The temporal relationship between host cell death, phytoalexin accumulation and fungal inhibition during hypersensitive reactions of Phaseolus vulgaris to Colletotrichum lindemuthianum. Physiol Plant Pathol 17:329–339Google Scholar
  4. Bennett MH, Gallagher MDS, Bestwick CS, Rossiter JT, Mansfield JW (1994) The phytoalexin response of lettuce to challenge by Botrytis cinerea, Bremia lactucae and Pseudomonas syringae pv. phaseolicola. Physiol Mol Plant Pathol 44:321–333CrossRefGoogle Scholar
  5. Bestwick CS, Bennett MH, Mansfield JW (1995) Hrp mutant of Pseudomonas syringae pv. phaseolicola induces cell wall alterations but not membrane damage leading to the hypersensitive reaction in lettuce. Plant Physiol 108:503–516PubMedGoogle Scholar
  6. Coyne DP, Schuster ML (1983) Genetics of and breeding for resistance to bacterial pathogens in vegetable crops. Hort-Science 18:30–36Google Scholar
  7. Dixon R, Lamb C (1990) Molecular communication in interactions between plants and microbial pathogens. Annu Rev Plant Physiol Plant Mol Biol 41:339–367CrossRefGoogle Scholar
  8. Ebel J, Cosio EG (1994) Elicitors of plant defense responses. Int Rev Cytol 48:1–36Google Scholar
  9. Ferreira JJ, Campa A, Pérez-Vega E, Giraldez R (2008) Reaction of a bean germplasm collection against five races of Colletotrichum lindemuthianum identified in northern Spain and implications for breeding. Plant Dis 92:705–708CrossRefGoogle Scholar
  10. Fillingham A, Wood J, Bevan J, Crute I, Mansfield J, Taylor J, Vivian A (1992) Avirulence genes from P. s. pvs. phaseolicola and pisi confer specificity towards both host and non-host species. Physiol Mol Plant Pathol 40:1–15CrossRefGoogle Scholar
  11. Fourie D (1998) Characterization of halo blight races on dry bean in South Africa. Plant Dis 82:307–310CrossRefGoogle Scholar
  12. Fourie D (2002) Distribution and severity of bacterial diseases on dry beans (Phaseolus vulgaris L.) in South Africa. J Phytopathol 150:220–226CrossRefGoogle Scholar
  13. Gnanamanickam S, Patil S (1977a) Accumulation of anti-microbial isoflavanoids in hypersensitivly responding bean leaf tissues inoculated with Pseudomonas phaseolicola. Physiol Plant Pathol 10:159–168CrossRefGoogle Scholar
  14. Gnanamanickam S, Patil S (1977b) Phaseolitoxin suppresses bacterially induced hypersensitive reaction and phytoalexin synthesis in bean cultivars. Physiol Plant Pathol 10:169–179CrossRefGoogle Scholar
  15. Hammond-Kosack KE, Jones JDG (1996) Resistant gene-dependent plant defense responses. Plant Cell 8:1773–1791PubMedCrossRefGoogle Scholar
  16. Hargreaves J, Bailey J (1978) Phytoalexin production by hypocotyls of Phaseolus vulgaris in response to constitutive metabolites released by damaged bean cells. Physiol Plant Pathol 13:89–100CrossRefGoogle Scholar
  17. Harper S, Zewdie N, Brown I, Mansfield J (1987) Histological, physiological and genetical studies of the responses of leaves and pods of Phaseolus vulgaris to three races of Pseudomonas syringae pv. phaseolicola and to P. s. pv. coronafaciens. Physiol Mol Plant Pathol 31:153–172CrossRefGoogle Scholar
  18. Hitchin F, Jenner C, Harper S, Mansfield J, Barber C, Daniels M (1989) Determination of cultivar specific avirulence cloned from Pseudomonas syringae pv. phaseolicola race 3. Physiol Mol Plant Pathol 34:309–322CrossRefGoogle Scholar
  19. Jenner C, Hitchin E, Mansfield J, Walters K, Betterridge P, Teverson D, Taylor J (1991) Gene for gene interactions between Pseudomonas syringae pv. phaseolicola and Phaseolus. Mol Plant Microb Interact 4:553–562CrossRefGoogle Scholar
  20. Lamb C, Lawton M, Dron M, Dixon R (1989) Signals and transduction mechanisms for activation of plant defences against microbial attack. Cell 56:215–224PubMedCrossRefGoogle Scholar
  21. Mansfield JW (1982) The role of phytoalexins in disease resistance. In: Bailey JA, Mansfield JW (eds) Phytoalexins. Blackie, Glasgow, pp 253–288Google Scholar
  22. Mansfield JW (2000) Antimicrobial compounds and resistance: the role of phytoalexins and phytoanticipins. In: Slusarenko AJ, Fraser RSS, Van Loon LC (eds) Mechanisms of resistance to plant diseases. Kluwer, Dordrecht, pp 325–370Google Scholar
  23. Mansfield J, Bennett M, Bestwick C, Woods-Tör A (1997) Phenotypic expression of gene-for-gene interaction involving fungal and bacterial pathogens: variation from recognition to response. In: Crute IR, Holub EB, Burdon JJ (eds) The gene-for-gene relationship in plant-parasite interactions. CAB International, Wallingford, pp 265–291Google Scholar
  24. Mills LJ, Silbernagel MJ (1992) A rapid screening technique to combine resistance to halo blight and bean common mosaic virus in Phaseolus vulgaris L. Euphytica 58:201–208CrossRefGoogle Scholar
  25. O’Brien F, Wood R (1973) Anti-bacterial substance in hypersensitive responses induced by bacteria. Nature 242:352–353Google Scholar
  26. Osbourn AE (1996) Preformed antimicrobial compounds and plant defense against fungal attack. Plant Cell 8:1821–1831PubMedCrossRefGoogle Scholar
  27. Pastor-Corrales MA, Otoya MM, Molina A, Singh SP (1995) Resistance to Colletotrichum lindemuthianum isolates from Middle America and Andean South America in different common bean races. Plant Dis 79:63–67CrossRefGoogle Scholar
  28. Pastor-Corrales MA, Jara C, Singh SP (1998) Pathogenic variation in, sources of, and breeding for resistance to Phaeoisariopsis griseola causing angular leaf spot in common bean. Euphytica 103:161–171CrossRefGoogle Scholar
  29. Paxton JD (1981) Phytoalexins—a working redefinition. Phytopathology 101:106–109CrossRefGoogle Scholar
  30. Saettler AW (1991) Diseases caused by bacteria. In: Hall R (ed) Compendium of bean diseases. The American Phytopathological Society, St. Paul, pp 29–32Google Scholar
  31. Smith CJ (1996) Accumulation of phytoalexins: defense mechanism and stimulus response system. New Phytol 132:1–45CrossRefGoogle Scholar
  32. Soriano-Richards E, Uribe-Salas D, Ibarra-Barrera G (1998) Phaseollin stored in vacuoles an phytoalexin response in bean. Plant Pathol 74:480–485CrossRefGoogle Scholar
  33. Soylu S (2006) Accumulation of cell-wall bound phenolic compounds and phytoalexin in Arabidopsis thaliana leaves following inoculation with pathovars of Pseudomonas syringae. Plant Sci 170:942–952CrossRefGoogle Scholar
  34. Soylu S, Bennett MH, Mansfield JW (2002) Induction of phytoalexin accumulation in broad bean (Vicia faba L.,) cotyledons following treatments with biotic and abiotic elicitors. Turk J Agric For 26:343–348Google Scholar
  35. Taylor JD, Phelps K, Dudley CL (1979) Epidemiology and strategy for the control of halo blight of bean. Ann Appl Biol 93:167–172CrossRefGoogle Scholar
  36. Taylor JD, Teverson DM, Allen DJ, Pastor-Corrales MA (1996a) Identification and origin of races of Pseudomonas syringae pv. phaseolicola from Africa and other bean growing areas. Plant Pathol 45:469–478CrossRefGoogle Scholar
  37. Taylor JD, Teverson DM, Davis JHC (1996b) Sources of resistance to Pseudomonas syringae pv phaseolicola races in Phaseolus vulgaris. Plant Pathol 45:479–485CrossRefGoogle Scholar
  38. Wagara N, Mwang’ombe AW, Kimenju JW, Buruchara RA, Kimani PM (2003) Pathogenic variability in Phaeoisariopsis griseola and response of bean germplasm to different races of the pathogen. Afr Crop Sci Conf Proc 6:352–357Google Scholar
  39. Webster D, Sequeira L (1977) Expression of resistance in bean pods to an incompatible isolate of Pseudomonas syringae. Can J Bot 55:2043–2052CrossRefGoogle Scholar
  40. Webster DM, Atkin JD, Cross JE (1983) Bacterial blights of snap beans and their control. Plant Dis 67:935–940CrossRefGoogle Scholar
  41. Yaish MWF, Sosa D, Vences FJ, Vaquero F (2006) Genetic mapping of quantitative resistance to race 5 of Pseudomonas syringae pv. phaseolicola in common bean. Euphytica 152:397–404CrossRefGoogle Scholar
  42. Yoshikawa M, Yamauchi K, Masago H (1978) Glyceollin: its role in restricting fungal growth in resistance soybean hypocotyls infected with Phytophthora megasperma var. sojae. Physiol Plant Pathol 12:73–82CrossRefGoogle Scholar
  43. Zaiter HZ, Coyne DP (1984) Testing inoculation methods and sources of resistance to the halo blight bacterium (Pseudomonas syringae pv. phaseolicola) in Phaseolus vulgaris. Euphytica 33:133–141CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.Department of Plant ProtectionMustafa Kemal University, Faculty of AgricultureAntakya, HatayTurkey

Personalised recommendations