, Volume 177, Issue 2, pp 267–275 | Cite as

Diagnostic value of molecular markers linked to the eyespot resistance gene Pch1 in wheat

  • Nina Meyer
  • Volker Lind
  • Magdalena Heindorf
  • Viktor Korzun
  • Wolfgang Friedt
  • Frank Ordon


Eyespot is a major disease of common wheat (Triticum aestivum L.) in temperate climates and causes yield losses of up to 40%. The causal agents of eyespot are Oculimacula acuformis (syn. Tapesia acuformis; anamorph: Helgardia acuformis syn. Pseudocercosporella herpotrichoides var. acuformis) and O. yallundae (syn. T. yallundae; anamorph: H. yallundae syn. P. h. var. herpotrichoides). Pch1 located on chromosome 7DL is the most important and most effective resistance gene, but its use in practical breeding is limited because of difficulties in phenotyping and the fact that markers like XustSSR2001-7DL are often population-specific in German wheat cultivars. Therefore, based on results obtained for endopeptidase Ep-D1a, which is very closely linked to Pch1, molecular markers located in the terminal region of chromosome 7DL were analysed in three DH (double haploid) populations. In a next step these molecular markers were validated on a set of German winter wheat cultivars to obtain information on their usefulness for marker assisted selection (MAS). Based on the analysis of 127 DH-lines, linkage to Pch1 (Ep-D1) was obtained for Xorw1, Xorw5, Xorw6, Xcfd175, Xbarc76, Xwmc14, and Xcfa2040. Analyses of 104 German winter wheat cultivars showed that Xorw1, Xorw6 and the SSR Xcfd175 of these markers are well suited for MAS in German wheat breeding.


Triticum aestivum Oculimacula spp. DH Doubled haploid 



This research project (AiF no. 14475 BR/1) was financially supported by the Federal Ministry of Economics and Technology (BMWi), administered via the Arbeitsgemeinschaft industrieller Forschungsvereinigungen e.V. (AiF) and the Gemeinschaft zur Förderung der privaten deutschen Pflanzenzüchtung e.V. (GFP). We wish to thank Karin Ernst for excellent technical assistance. We also thank the companies KWS Lochow GmbH and Deutsche Saatveredelung AG for providing marker data and plant material. We would like to thank Dr. Robert McIntosh and the unknown reviewers for helpful suggestions to improve the manuscript.


  1. Bang R (1986) Experimentelle Untersuchungen zur genetischen Analyse und zur Verbesserung der Widerstandsfähigkeit des Saatweizens Triticum aestivum L. gegenüber Pseudocercosporella herpotrichoides (Fron) Deighton. Dissertation, Martin-Luther-Universität Halle-Wittenberg, GermanyGoogle Scholar
  2. Bierman SM, Fitt BDL, van den Bosch F, Bateman GL, Jenkyn JF, Welham SJ (2002) Changes in populations of the eyespot fungi Tapesia yallundae and T. acuformis under different fungicide regimes in successive crops of winter wheat, 1984–2000. Plant Pathol 51:191–201CrossRefGoogle Scholar
  3. Bockus WW, Su Z, Garrett KA, Gill BS, Stack P, Fritz AK, Roozeboom KL, Martin TJ (2007) Numbers of experiments needed to determine wheat disease phenotypes for four wheat diseases. Plant Dis 91:103–108CrossRefGoogle Scholar
  4. Bundessortenamt (1994, 2003, 2004, 2007, 2008) Beschreibende Sortenliste. Getreide, Mais, Ölfrüchte, Leguminosen (großkörnig), Hackfrüchte (außer Kartoffeln). Landbuchverlag, Hannover, GermanyGoogle Scholar
  5. Chapman NH, Burt C, Dong H, Nicholson P (2008) The development of PCR-based markers for the selection of eyespot resistance genes Pch1 and Pch2. Theor Appl Genet 117:425–433CrossRefPubMedGoogle Scholar
  6. Chapman NH, Burt C, Nicholson P (2009) The identification of candidate genes associated with Pch2 eyespot resistance in wheat using cDNA-AFLP. Theor Appl Genet 118:1045–1057CrossRefPubMedGoogle Scholar
  7. Crous PW, Groenewald JZ, Gams W (2003) Eyespot of cereals revisited: ITS phylogeny reveals new species relationships. Eur J Plant Pathol 109:841–950CrossRefGoogle Scholar
  8. de la Peña RC, Murray TD, Jones SS (1996) Linkage relations among eyespot resistance gene Pch2, endopeptidase Ep-A1b, and RFLP marker Xpsr121 on chromosome 7A of wheat. Plant Breed 115:273–275CrossRefGoogle Scholar
  9. de la Peña RC, Murray TD, Jones SS (1997) Identification of an RFLP interval containing Pch2 on chromosome 7AL in wheat. Genome 40:249–252CrossRefGoogle Scholar
  10. Doussinault G, Dosba F (1977) An investigation into increasing the variability for resistance to eyespot in wheat. Eyespot-variability in the subtribe Triticinae. Z Pflanzenzücht 79:122–133Google Scholar
  11. Freier U (1982) Prüfung von Aegilops-Arten auf Resistenz gegen Pseudocercosporella herpotrichoides (Fron) Deighton. Dissertation, Martin-Luther-Universität Halle-Wittenberg, GermanyGoogle Scholar
  12. Groenewald JZ, Marais AS, Marais GF (2003) Amplified fragment length polymorphism-derived microsatellite sequence linked to the Pch1 and Ep-D1 loci in common wheat. Plant Breed 122:83–85CrossRefGoogle Scholar
  13. Lange-de la Camp M (1966) Die Wirkungsweise von Cercosporella herpotrichoides Fron, dem Erreger der Halmbruchkrankheit des Getreides, III. Art und Ausmaß des Schadens–Bekämpfungsmöglichkeiten. Phytopathol Z 56:363–392CrossRefGoogle Scholar
  14. Langridge P, Chalmers K (2005) The principle: identification and application of molecular markers. In: Lorz H, Wenzel G (eds) Biotechnology in agriculture and forestry. Molecular marker systems, vol 55. Springer, Heidelberg, pp 3–22Google Scholar
  15. Law CN, Scott PR, Worland AJ, Hollins TW (1976) The inheritance of resistance to eyespot (Cercosporella herpotrichoides) in wheat. Genet Res Camb 25:73–79Google Scholar
  16. Leonard JM, Watson CJW, Carter AH, Hansen JL, Zemetra RS, Santra DK, Campbell KG, Riera-Lizarazu O (2008) Identification of a candidate gene for the wheat endopeptidase Ep-D1 locus and two other STS markers linked to the eyespot resistance gene Pch1. Theor Appl Genet 116:261–270CrossRefPubMedGoogle Scholar
  17. Li HJ, Arterburn M, Jones SS, Murray TD (2004) A new source of resistance to Tapesia yallundae associated with a homoeologous group 4 chromosome in Thinopyrum ponticum. Phytopathology 94:932–937CrossRefPubMedGoogle Scholar
  18. Li HJ, Arterburn M, Jones SS, Murray TD (2005) Resistance to eyespot of wheat, caused by Tapesia yallundae derived from Thinopyrum intermedium homoelogous group 4 chromosome. Theor Appl Genet 111:932–940CrossRefPubMedGoogle Scholar
  19. Lind V (1999) Variation of resistance to Pseudocercosporella herpotrichoides (Fron) deighton in wheat genotypes carrying the gene Pch-1. Plant Breed 118:281–287CrossRefGoogle Scholar
  20. Maia N (1967) Obtention de blés tendres résistants au piétin-verse par croisements interspécifiques blés × Aegilops. C R Acad Agric Fr 53:149–154Google Scholar
  21. McMillin DE, Allan RE, Roberts DE (1986) Association of an isozyme locus and strawbreaker foot rot resistance derived from Aegilops ventricosa in wheat. Theor Appl Genet 72:743–747CrossRefGoogle Scholar
  22. Melchinger AE (1990) Use of molecular markers in breeding for oligogenic disease resistance. Plant Breed 104:1–19CrossRefGoogle Scholar
  23. Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832CrossRefPubMedGoogle Scholar
  24. Mielke H (1995) Studien zum Befall des Weizens mit Pseudocercosporella herpotrichoides (Fron) Deighton unter Berücksichtigung der Sorten- und Artenanfälligkeit sowie der Bekämpfung des Erregers. Mitteil Biol Bundesanst Land- u Forstwirtsch Berlin-Dahlem 314:197Google Scholar
  25. Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325CrossRefPubMedGoogle Scholar
  26. Ordon F, Habekuss A, Kastirr U, Rabenstein F, Kühne T (2009) Virus resistance in cereals: sources of resistance, genetics and breeding. J Phytopathol 157:535–545CrossRefGoogle Scholar
  27. Santra DK, Watt C, Little L, Kidwell KK, Campbell KG (2006) Comparisom of a modified assay method for the endopeptidase marker Ep-D1b with the sequence tag site marker XustSSR2001-7DL for strawbreaker foot rot resistance in wheat. Plant Breed 125:13–18CrossRefGoogle Scholar
  28. Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114CrossRefPubMedGoogle Scholar
  29. Vahl U, Müller G, Thiele A, Thiele M (1987) Multiple endopeptidasen als biochemische marker für die resistenz von winterweizen gegenüber pseudocercosporella herpotrichoides (Fron) Deighton. Plant Breed 99:218–225CrossRefGoogle Scholar
  30. Van Ooijen JW (2006) JoinMap® 4. Software for the calculation of genetic linkage maps in experimental populations. Plant Research International, WageningenGoogle Scholar
  31. Van Ooijen JW, Boer MP, Jansen RC, Maliepaard C (2002) MapQTL® 4.0. Software for the calculation of QTL positions on genetic maps. Plant Research International, WageningenGoogle Scholar
  32. William HM, Trethowan R, Crosby-Galvan FM (2007) Wheat breeding assisted by markers: CIMMYT’s experience. Euphytica 157:307–319CrossRefGoogle Scholar
  33. Yildirim A, Jones SS, Murray TD (1998) Mapping a gene conferring resistance to Pseudocercosporella herpotrichoides on chromosome 4 V of Dasypyrum villosum in a wheat background. Genome 41:1–6CrossRefGoogle Scholar
  34. Yildirim A, Jones SS, Murray TD, Line RF (2000) Evaluation of Dasypyrum villosum populations for resistance to cereal eyespot and stripe rust pathogens. Plant Dis 84:40–44CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Nina Meyer
    • 1
  • Volker Lind
    • 1
  • Magdalena Heindorf
    • 1
  • Viktor Korzun
    • 2
  • Wolfgang Friedt
    • 3
  • Frank Ordon
    • 1
  1. 1.Institute for Resistance Research and Stress Tolerance, Julius Kuehn-Institute (JKI)Federal Research Centre for Cultivated PlantsQuedlinburgGermany
  2. 2.KWS LOCHOW GMBHBergenGermany
  3. 3.Plant Breeding DepartmentResearch Centre for Bio Systems Land Resources and Nutrition (IFZ), Justus-Liebig-University GiessenGiessenGermany

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