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A QTL controlling low temperature induced spikelet sterility at booting stage in rice

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Abstract

Low temperature is a major abiotic stress for rice cultivation, causing serious yield loss in many countries. To identify QTL controlling low temperature induced spikelet sterility in rice, the progeny of F2, BC1F1 and BC2F1 populations derived from a Reiziq × Lijiangheigu cross were exposed to 21/15°C for 15 days at the booting stage, and spikelet sterility was assessed. For genotyping, 92 polymorphic markers from 373 SSR and 325 STS primer pairs were used. A major QTL was initially indentified on the short arm of chromosome 10 by selective genotyping using highly tolerant and susceptible progeny from F2 and BC1F1 populations. The QTL (qLTSPKST10.1) was validated and mapped by genotyping the entire F2 (282 progeny) and BC1F1 (84 progeny) populations. The results from the F2 population showed that qLTSPKST10.1 could explain 20.5% of the variation in spikelet sterility caused by low temperature treatment with additive (a = 14.4) and dominant effect (d = −7.5). From the analysis of 98 selected BC2F1 progeny, the QTL located in the 3.5 cM interval between S10010.9 and S10014.4 was further confirmed. Based on the studies of 3 generations in 2 years, it was clear that the QTL on chromosome 10 is a major determinant of the control of low temperature induced spikelet sterility at booting stage.

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References

  • Abe N, Kotaka S, Toriyama K, Kobayashi M (1989) Development of the “Rice Norin-PL8” with high tolerance to cool temperature at the booting stage. Res Bull Hokkaido Agric Exp Stn 152:9–17

    Google Scholar 

  • Andaya VC, Mackill DJ (2003) QTLs conferring cold tolerance at the booting stage of rice using recombinant inbred lines from a japonica x indica cross. Theor Appl Genet 106:1084–1090

    CAS  PubMed  Google Scholar 

  • Burr K, Harper R, Linacre A (2001) One-step isolation of plant DNA suitable for PCR amplification. Plant Mol Biol Report 19:367–371

    Article  CAS  Google Scholar 

  • Chen M, Presting G et al (2002) An integrated physical and genetic map of the rice genome. Plant Cell 14:537–545

    Article  PubMed  Google Scholar 

  • Chin J, Kim J, Jiang W, Chu S, Woo M, Han L, Brar D, Koh H (2007) Identification of subspecies-specific STS markers and their association with segregation distortion in rice (Oryza sativa L.). J Crop Sci Biotechnol 10:175–184

    Google Scholar 

  • Churchill G, Doerg R (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971

    CAS  PubMed  Google Scholar 

  • Dai L, Ye C, Yu T, Xu F (2002) Studies on cold tolerance of rice, Oryza sativa L.I.Description on types of cold injury and classifications of evaluation methods on cold tolerance in rice. Southwest Chin J Agric Sci 15:41–45

    Google Scholar 

  • Dai LY, Lin XH, Ye CR, Ise KZ, Saito K, Kato A, Xu FR, Yu TQ, Zhang DP (2004) Identification of quantitative trait loci controlling cold tolerance at the reproductive stage in Yunnan landrace of rice, Kunmingxiaobaigu. Breed Sci 54:253–258

    Article  CAS  Google Scholar 

  • Farrell TC, Fox KM, Williams RL, Fukai S, Lewin LG (2006) Minimising cold damage during reproductive development among temperate rice genotypes. II. Genotypic variation and flowering traits related to cold tolerance screening. Aust J Agric Res 57:89–100

    Article  Google Scholar 

  • Han L, Zhang S (2004) Methods of characterization and evaluation of cold tolerance in rice. J Plant Genet Resour 5:75–80

    Google Scholar 

  • Jia J, Zhang D, Li C, Qu X, Wang S, Chamarerk V, Nguyen H, Wang B (2001) Molecular mapping of the reverse thermo-sensitive genic male-sterile gene (rtms1) in rice. Theor Appl Genet 103:607–612

    Article  CAS  Google Scholar 

  • Lander E, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185–199

    CAS  PubMed  Google Scholar 

  • Li H, Wang J, Liu A, Liu K, Zhang Q, Zou J (1997) Genetic basis of low-temperature-sensitive sterility in indica-japonica hybrids of rice as determined by RFLP analysis. Theor Appl Genet 95:1092–1097

    Article  CAS  Google Scholar 

  • Lin M, Lin C et al (2007) Tagging rice drought-related QTL with SSR DNA markers. Crop Environ Bioinform 4:65–76

    CAS  Google Scholar 

  • Liu FX, Sun CQ, Tan LB, Fu YC, Li DJ, Wang XK (2003) Identification and mapping of quantitative trait loci controlling cold-tolerance of Chinese common wild rice (O-rufipogon Griff.) at booting to flowering stages. Chin Sci Bull 48:2068–2071

    Article  Google Scholar 

  • McCouch SR, CGSNL (2008) Gene nomenclature system for rice. Rice 1:72–84

    Article  Google Scholar 

  • McCouch S, Teytelman L et al (2002) Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.). DNA Res 9:199–207

    Article  CAS  PubMed  Google Scholar 

  • Saito K, Miura K, Nagano K, Hayano-Saito Y, Saito A, Araki H, Kato A (1995) Chromosomal location of quantitative trait loci for cool tolerance at the booting stage in rice variety ‘Norin-PL8’. Breed Sci 45:337–340

    Google Scholar 

  • Saito K, Miura K, Nagano K, Hayano-Saito Y, Araki H, Kato A (2001) Identification of two closely linked quantitative trait loci for cold tolerance on chromosome 4 of rice and their association with anther length. Theor Appl Genet 103:862–868

    Article  CAS  Google Scholar 

  • Saito K, Hayano-Saito Y, Maruyama-Funatsuki W, Sato Y, Kato A (2004) Physical mapping and putative candidate gene identification of a quantitative trait locus Ctb1 for cold tolerance at the booting stage of rice. Theor Appl Genet 109:515–522

    Article  CAS  PubMed  Google Scholar 

  • Satake T (1976) Determination of the most sensitive stage to sterile-type cool injury in rice plants. Res Bull Hokkaido Natl Agric Exp Stn 113:1–33

    Google Scholar 

  • Takeuchi Y, Hayasaka H, Chiba B, Tanaka I, Shimano T, Yamagishi M, Nagano K, Sasaki T, Yano M (2001) Mapping quantitative trait loci controlling cool-temperature tolerance at the booting stage in temperate japonica rice. Breed Sci 51:191–197

    Article  CAS  Google Scholar 

  • The Rice Chromosome 10 Sequencing Consortium (2003) In-depth view of structure, activity, and evolution of rice chromosome 10. Science 300:1566–1569

    Article  Google Scholar 

  • Wang S, Basten C, Zeng Z (2007) Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh, NC

  • Xu L, Zhou L, Zeng Y, Wang F, Zhang H, Shen S, Li Z (2008) Identification and mapping of quantitative trait loci for cold tolerance at the booting stage in a japonica rice near-isogenic line. Plant Sci 174:340–347

    Article  CAS  Google Scholar 

  • Ye C, Fukai S, Godwin I, Reinke R, Snell P, Schiller J, Basnayake J (2009) Cold tolerance in rice varieties at different growth stages. Crop Pasture Sci 60:1–11

    Article  Google Scholar 

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Acknowledgments

This study was supported by a postdoctoral fellowship and a travel grant from University of Queensland. The authors also appreciate the assistance from the staff and postgraduate students in Molecular Genetics Laboratory at University of Queensland and Rice Genomics & Breeding Laboratory at Seoul National University.

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Authors and Affiliations

  1. School of Land, Crop and Food Sciences, University of Queensland, Brisbane, QLD, 4072, Australia

    C. Ye, S. Fukai, I. D. Godwin, Y. Zhou & C. Lambrides

  2. Department of Plant Science, Seoul National University, Seoul, 151-921, South Korea

    H. Koh & W. Jiang

  3. Department of Industry and Investment NSW, Yanco Agricultural Institute, Orange, NSW, 2703, Australia

    R. Reinke & P. Snell

  4. International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines

    C. Ye & E. Redoña

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  1. C. Ye
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  2. S. Fukai
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  3. I. D. Godwin
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  4. H. Koh
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  5. R. Reinke
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  6. Y. Zhou
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  7. C. Lambrides
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  8. W. Jiang
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  9. P. Snell
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  10. E. Redoña
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Correspondence to C. Ye.

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Ye, C., Fukai, S., Godwin, I.D. et al. A QTL controlling low temperature induced spikelet sterility at booting stage in rice. Euphytica 176, 291–301 (2010). https://doi.org/10.1007/s10681-010-0226-8

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  • DOI: https://doi.org/10.1007/s10681-010-0226-8

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