Skip to main content
SpringerLink
Log in

Identification and sequence characterisation of molecular markers polymorphic between male kiwifruit (Actinidia chinensis var. deliciosa (A. Chev.) A. Chev.) accessions exhibiting different flowering time

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Fruit set in kiwifruit is strongly dependent on pollination, which is limited by the lack of efficient male pollen donors, among other factors. We searched for molecular markers that could be polymorphic in relation to flowering time in order to classify male kiwifruit plants to discard those that are not likely to perform as efficient pollen donors. Random amplified polymorphic DNA (RAPD) and modified amplified fragment length polymorphism (AFLP) markers were generated using 41 male kiwifruit plants in two flowering groups, early- and late-flowering males (with respect to the female cultivar ‘Hayward’). One RAPD and nine modified-AFLP markers polymorphic between male plants exhibiting different flowering time were identified, sequenced and analysed in databases. Unweighted pair group method with arithmetic average (UPGMA) clustering and multidimensional scaling showed that these markers could be used to classify the male plants into flowering groups. Analysis of molecular variance (AMOVA) agreed with this classification, showing that most of the genetic variation is found between flowering groups. Sequence analysis based on a database search revealed that the polymorphism PolM contains a 7-nucleotide long element involved in the repression of the phytochrome A gene, that Pol4 is a partial sequence of a phytochrome B gene, and that sequences Pol3, Pol5, Pol7, and Pol9 show high identity with ESTs from kiwifruit buds treated with hydrogen cyanamide. Clustering analysis supported the previous classification of males into flowering groups, making it feasible to predict male plants’ flowering times with respect to the cultivar ‘Hayward’ based upon these molecular markers.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410

    CAS  PubMed  Google Scholar 

  • Bañuelos GR, Argumedo R, Patel K, Ng V, Zhou F, Vellanoweth RL (2008) The developmental transition to flowering in Arabidopsis is associated with an increase in leaf chloroplastic lipoxygenase activity. Plant Sci 174:366–373. doi:10.1016/j.plantsci.2007.12.009

    Article  PubMed  Google Scholar 

  • Böhlenius H, Huang T, Charbonnel-Campaa L, Brunner AM, Jansson S, Strauss SH, Nilsson O (2007) CO/FT regulatory module controls timing of flowering and seasonal growth cessation in trees. Science 312:1040–1043. doi:10.1126/science.1126038

    Article  Google Scholar 

  • Brendel V, Xing L, Zhu W (2004) Gene structure prediction from consensus spliced alignment of multiple ESTs matching the same genomic locus. Bioinformatics 20:1157–1169. doi:10.1093/bioinformatics/bth058

    Article  CAS  PubMed  Google Scholar 

  • Bruce MB, Deng XW, Quail PH (1991) A negatively acting DNA sequence element mediates phytochrome-directed regression of phyA gene transcription. EMBO J 10:3015–3024

    CAS  PubMed  Google Scholar 

  • Cipriani G, Di Bella R, Testolin R (1996) Screening RAPD primers for molecular taxonomy and cultivar fingerprinting in the genus Actinidia. Euphytica 90:169–174. doi:10.1007/BF00023855

    Article  CAS  Google Scholar 

  • Cipriani G, Testolin R, Gardner R (1998) Restriction-site variation of PCR-amplified chloroplast DNA regions and its implication for the evolution and taxonomy of Actinidia. Theor Appl Genet 96:389–396. doi:10.1007/s001220050754

    Article  CAS  Google Scholar 

  • Crowhurst RN, Gleave AP, MacRae EA et al (2008) Analysis of expressed sequence tags from Actinidia: applications of a cross species EST database for gene discovery in the areas of flavor, health, color and ripening. BMC Genomics 9:351. doi:10.1186/1471-2164-9-351

    Article  PubMed  Google Scholar 

  • Cutler RW, Chundet R, Handa T, Anuntalabhochai S (2006) Development of sequence characterized DNA markers linked to a temperate dependence for flower induction in lychee (Litchi chinensis Sonn.) cultivars. Sci Hortic 107:264–270. doi:10.1016/j.scienta.2005.08.005

    Article  CAS  Google Scholar 

  • Excoffier L, Smouse PE, Quattro JM (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131:479–491

    CAS  PubMed  Google Scholar 

  • Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinform Online 1:47–50

    CAS  PubMed  Google Scholar 

  • Ferguson AR (2007) The need for characterisation and evaluation of germplasm: kiwifruit as an example. Euphytica 154:371–382. doi:10.1007/s10681-006-9188-2

    Article  Google Scholar 

  • Fraser LG, Harvey CF, Crowhurst RN, De Silva HN (2004) EST-derived microsatellites from Actinidia species and their potential for mapping. Theor Appl Genet 108:1010–1016. doi:10.1007/s00122-003-1517-4

    Article  CAS  PubMed  Google Scholar 

  • Fraser LG, McNeilage MA, Tsang GK, Harvey CF, De Silva HN (2005) Cross-species amplification of microsatellite loci within the dioecious, polyploid genus Actinidia (Actinidiaceae). Theor Appl Genet 112:149–157. doi:10.1007/s00122-005-0117-x

    Article  CAS  PubMed  Google Scholar 

  • Fraser LG, Tsang GK, Datson PM, De Silva HN, Harvey CF, Gill GP, Crowhurst RN, McNeilage MA (2009) A gene-rich linkage map in the dioecious species Actinidia chinensis (kiwifruit) reveals putative X/Y sex-determining chromosomes. BMC Genomics 10:102. doi:10.1186/1471-2164-10-102

    Article  PubMed  Google Scholar 

  • Gill GP, Harvey CF, Gardner RC, Fraser LG (1998) Development of sex-linked PCR markers for gender identification in Actinidia. Theor Appl Genet 97:439–445. doi:10.1007/s001220050914

    Article  CAS  Google Scholar 

  • González MV, Coque M, Herrero M (1994) Pollinator selection in kiwifruit (Actinidia deliciosa). J Hortic Sci 70:375–387

    Google Scholar 

  • González MV, Coque M, Herrero M (1995a) Stigmatic receptivity limits the effective pollination period in kiwifruit. J Am Soc Hortic Sci 120:199–202

    Google Scholar 

  • González MV, Coque M, Herrero M (1995b) Papillar integrity as an indicator of stigmatic receptivity in kiwifruit (Actinidia deliciosa). J Exp Bot 46:263–269

    Article  Google Scholar 

  • Harvey CF, Gill GP, Fraser LG, McNeilage MA (1997) Sex determination in Actinidia. 1. Sex-linked markers and progeny sex ratio in diploid A. chinensis. Sex Plant Reprod 10:149–154. doi:10.1007/s004970050082

    Article  Google Scholar 

  • Huang W-G, Cipriani G, Morgante M, Testolin R (1998) Microsatellite DNA in Actinidia chinensis: isolation, characterisation, and homology in related species. Theor Appl Genet 97:1269–1278. doi:10.1007/s001220051019

    Article  CAS  Google Scholar 

  • Huang H, Li Z, Li J, Kubisiak TL, Layne DR (2002) Phylogenetic relationships in Actinidia as revealed by RAPD analysis. J Am Soc Hortic Sci 127:759–766

    CAS  Google Scholar 

  • Kobayashi S, Goto-Yamamoto N, Hirochika H (2004) Retrotransposon-induced mutations in grape skin color. Science 304:982. doi:10.1126/science.1095011

    Article  PubMed  Google Scholar 

  • Korkovelos AE, Mavromatis AG, Huang WG, Hagidimitriou M, Giakoundis A, Goulas CK (2008) Effectiveness of SSR molecular markers in evaluating the phylogenetic relationships among eight Actinidia species. Sci Hortic 116:305–310. doi:10.1016/j.scienta.200801.011

    Article  CAS  Google Scholar 

  • Kumar A, Hirochika H (2001) Applications of retrotransposons as genetic tools in plant biology. Trends Plant Sci 6:127–134. doi:10.1016/S1360-1385(00)01860-4

    Article  CAS  PubMed  Google Scholar 

  • Lin C (2000) Photoreceptors and regulation of flowering time. Plant Physiol 123:39–50

    Article  CAS  PubMed  Google Scholar 

  • McPherson HG, Richardson AC, Snelgar WP, Currie MB (2001) Effects of hydrogen cyanamide on budbreak and flowering in kiwifruit (Actinidia deliciosa ‘Hayward’). N Z J Crop Hortic Sci 29:277–285

    CAS  Google Scholar 

  • Mockler T, Yang H, Yu X, Parikh D, Cheng Y, Dolan S, Lin C (2003) Regulation of photoperiodic flowering by Arabidopsis photoreceptors. Proc Natl Acad Sci USA 100:2140–2145. doi:10.1073/pnas.0437826100

    Article  CAS  PubMed  Google Scholar 

  • Negi MS, Devic M, Delseny M, Lakshmikumaran M (2000) Identification of AFLP fragments linked to seed coat colour in Brassica juncea and conversion to a SCAR marker for rapid selection. Theor Appl Genet 101:281–285. doi:10.1007/s001220051463

    Article  Google Scholar 

  • Noguera FJ, Capel J, Alvarez JI, Lozano R (2005) Development and mapping of a codominant SCAR marker linked to the andromonoecious gene of melon. Theor Appl Genet 110:714–720. doi:10.1007/s00122-004-1897-0

    Article  CAS  PubMed  Google Scholar 

  • Nybom H, Hall HK (1991) Minisatellite DNA ‘fingerprints’ can distinguish Rubus cultivars and estimate their degree of relatedness. Euphytica 53:107–114. doi:10.1007/BF00023790

    Article  CAS  Google Scholar 

  • Palombi MA, Damiano C (2002) Comparison between RAPD and SSR molecular markers in detecting genetic variation in kiwifruit (Actinidia deliciosa A. Chev.). Plant Cell Rep 20:1061–1066. doi:10.1007/s00299-001-0430-z

    Article  CAS  Google Scholar 

  • Pearson WR, Lipman DJ (1988) Improved tools for biological sequence analysis. Proc Natl Acad Sci USA 85:2444–2448

    Article  CAS  PubMed  Google Scholar 

  • Prado MJ, Herrera MT, Vazquez RA, Romo S, González MV (2005) Micropropagation of two selected male kiwifruit and analysis of genetic variation with AFLP markers. HortScience 40:740–746

    CAS  Google Scholar 

  • Prado MJ, Romo S, Novo M, Rey M, Herrera MT, González MV (2006) Molecular characterization of three commercial cultivars and a new pollinator in kiwifruit. HortScience 41:90–95

    CAS  Google Scholar 

  • Prado MJ, González MV, Romo S, Herrera MT (2007) Adventitious plant regeneration on leaf explants from adult male kiwifruit and AFLP analysis of genetic variation. Plant Cell Tissue Organ Cult 88:1–10. doi:10.1007/s11240-006-9116-0

    Article  Google Scholar 

  • Ranamukhaarachchi DG, Kane ME, Guy CL, Li KB (2000) Modified AFLP technique for rapid genetic characterization in plants. BioTechniques 29:858–866

    CAS  PubMed  Google Scholar 

  • Reed JW, Nagatani A, Elich TD, Fagan M, Chory J (1994) Phytochrome A and phytochrome B have overlapping but distinct functions in Arabidopsis development. Plant Physiol 104:1139–1149

    CAS  PubMed  Google Scholar 

  • Shirkot P, Sharma DR, Mohapatra T (2002) Molecular identification of sex in Actinidia deliciosa var. deliciosa by RAPD markers. Sci Hortic 94:33–39. doi:10.1016/S0304-4238(01)00357-0

    Article  CAS  Google Scholar 

  • Sneath PHA, Sokal RR (1973) Numerical taxonomy. The principles and practice of numerical classification. WH Freeman, San Francisco

    Google Scholar 

  • Testolin R, Cipriani G, Gottardo L, Costa G (1995) Valutazione de selezione maschili di actinidia come impollinatori per la cv. ‘Hayward’. Riv Fruttic Ortofloric 57:63–68

    Google Scholar 

  • Testolin R, Huang WG, Laín O, Messina R, Vecchione A, Cipriani G (2001) A kiwifruit (Actinidia spp.) linkage map based on microsatellites and integrated with AFLP markers. Theor Appl Genet 103:30–36. doi:10.1007/s00122-001-0555-z

    Article  CAS  Google Scholar 

  • Thorp TG, Zhang J, Lay-Yee M (1990) Horticultural characteristics of seven pistillate and three staminate New Zealand cultivars of kiwifruit. N Z J Crop Hortic Sci 18:233–240

    Google Scholar 

  • Urusaki N, Tokumoto M, Tarora K, Ban Y, Kayano T, Tanaka H, Oku H, Chinen I, Terauchi R (2002) A male and hermaphrodite specific RAPD marker for papaya (Carica papaya L.). Theor Appl Genet 104:281–285. doi:10.1007/s001220100693

    Article  Google Scholar 

  • Valverde F, Mouradov A, Soppe W, Ravenscroft D, Samach A, Coupland G (2004) Photoreceptor regulation of CONSTANS protein in photoperiodic flowering. Science 303:1003–1006. doi:10.1126/science.1091761

    Article  CAS  PubMed  Google Scholar 

  • Walton EF, Fowke PJ (1993) Effect of hydrogen cyanamide on kiwifruit shoot flower number and position. J Hortic Sci 68:529–534

    CAS  Google Scholar 

  • Walton EF, Podivinsky E, Wu R-M (2001) Bimodal patterns of floral gene expression over the two seasons that kiwifruit flowers develop. Physiol Plant 111:396–404. doi:10.1111/j.1399-3054.2001.1110318.x

    Article  CAS  PubMed  Google Scholar 

  • Weising K, Fung RWM, Keeling DJ, Atkinson RG, Gardner RC (1996) Characterisation of microsatellites from Actinidia chinensis. Mol Breed 2:117–131. doi:10.1007/BF00441427

    Article  CAS  Google Scholar 

  • Wingender E, Chen X, Hehl R, Karas H, Liebich I, Matys V, Meinhardt T, Prüß M, Reuter I, Schacherer F (2000) TRANSFAC: an integrated system for gene expression regulation. Nucleic Acids Res 28:316–319

    Article  CAS  PubMed  Google Scholar 

  • Xu W-J, Wang B-W, Cui K-M (2004) RAPD and SCAR markers linked to sex determination in Eucommia ulmoides Oliv. Euphytica 136:233–238. doi:10.1023/B:EUPH.0000032741.99885.c4

    Article  CAS  Google Scholar 

  • Yakubov B, Barazani O, Golan-Goldhirsh A (2005) Combination of SCAR primers and touchdown-PCR for sex identification in Pistacia vera L. Sci Hortic 103:473–478. doi:10.1016/j.scienta.2004.06.008

    Article  CAS  Google Scholar 

  • Ye Z, Rodríguez R, Tran A, Hoang H, de los Santos D, Brown S, Vellanoweth RL (2000) The developmental transition to flowering represses ascorbate peroxidase activity and induces enzymatic lipid peroxidation in leaf tissue in Arabidopsis thaliana. Plant Sci 158:115–127. doi:10.1016/S0168-9452(00)00316-2

    Article  CAS  PubMed  Google Scholar 

  • Yu K, Pauls KP (1994) Optimization of DNA-extraction and PCR procedures for random amplified polymorphic DNA (RAPD) analysis in plants. In: Griffin HG, Griffin AM (eds) PCR technology: current innovations. CRC Press Inc, Boca Raton, pp 93–100

    Google Scholar 

  • Zhang J, Thorp TG (1986) Morphology of nine pistillate and three staminate New Zealand clones of kiwifruit (Actinidia deliciosa (Chev.) Liang and Ferguson var. deliciosa). N Z J Bot 24:589–613

    Google Scholar 

  • Zhen Y, Li Z, Huang H (2004) Molecular characterization of kiwifruit (Actinidia) cultivars and selections using SSR markers. J Am Soc Hortic Sci 129:374–382

    CAS  Google Scholar 

Download references

Acknowledgments

Marina Novo and Silvia Romo contributed equally to this work. This is a contribution of the Interuniversity Research Group in Biotechnology and Reproductive Biology of Woody Plants. This research was supported by the Xunta de Galicia (Regional Government of Galicia, project PGIDIT04RAG291002PR). We thank SERIDA (Regional Government of the Principado de Asturias) and Kiwi Atlantico S.A. for providing the plant material, Drs. E. Labrador and B. Dopico (Univ. Salamanca, Spain) for letting us use their laboratory facilities, and Verónica López for her technical assistance. We also wish to thank Dr. Pablo Presa (Univ. Vigo, Spain) for his help with the AMOVA analysis.

Author information

Authors and Affiliations

  1. Departamento de Fisiología Vegetal, Universidad de Santiago de Compostela, Campus sur, 15872, Santiago de Compostela, Spain

    Marina Novo, Silvia Romo & María Victoria González

  2. Departamento de Biología Vegetal y Ciencia del Suelo, Universidad de Vigo, 36310, Vigo, Spain

    Manuel Rey & María Jesús Prado

Authors
  1. Marina Novo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Silvia Romo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Manuel Rey
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. María Jesús Prado
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. María Victoria González
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to María Victoria González.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Novo, M., Romo, S., Rey, M. et al. Identification and sequence characterisation of molecular markers polymorphic between male kiwifruit (Actinidia chinensis var. deliciosa (A. Chev.) A. Chev.) accessions exhibiting different flowering time. Euphytica 175, 109–121 (2010). https://doi.org/10.1007/s10681-010-0192-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-010-0192-1

Keywords

Search

Navigation