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Euphytica

, Volume 169, Issue 1, pp 57–68 | Cite as

A major QTL associated with preharvest sprouting in rapeseed (Brassica napus L.)

  • Faqiang Feng
  • Pingwu Liu
  • Dengfeng Hong
  • Guangsheng Yang
Article

Abstract

Preharvest sprouting (PHS) is one of the most important factors affecting the cereal production worldwide, in regions characterized by rainfall and high humidity during harvest season. It is sometimes a problem in rapeseed (Brassica napus L.), especially in production of commercial F1 hybrids. To detect quantitative trait loci (QTL) controlling PHS, a F2 population consisting of 269 F2:3 lines was created from the cross between a PHS-tolerant line (117AB) and a PHS-susceptible line (7,605). A linkage map was constructed using 35 Simple Sequence Repeat markers and 242 Amplified Fragment Length Polymorphism markers. PHS was measured as a percentage of sprouted seeds on the mother plant, 7 days after physiological maturity. Five putative QTLs for PHS were detected and located on LG2 (N11) and LG7 (N3), respectively. Phenotypic variance explained by each QTL ranged from 4.11 to 50.78% and the five putative QTLs explained about 75.63% of the total phenotypic variance. A major QTL was identified on LG2 (N11) flanked by P3C4180 and C6C13160, which explained 50.78% of the total phenotypic variance. Meanwhile, we detected four significant epistatic interactions with a total contribution of 17.16% of the total phenotypic variance.

Keywords

Brassica napus Preharvest sprouting (PHS) Quantitative trait loci (QTL) 

Notes

Acknowledgments

This research was supported by funding from the National Key Technology Research and Development Program (No. 2006BAD01A04), Program for Changjiang Scholars and Innovation Research Team in University (No. IRT0442), the National High Technology Research and Development Program of China (863 Program) (No.2006AA10Z1B8), the Program for “948” (2003-Q04) and Double Low Rapeseed Cultivar Breeding Project from Hubei Provincial Government (2006AA206A03).

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Faqiang Feng
    • 1
  • Pingwu Liu
    • 1
  • Dengfeng Hong
    • 1
  • Guangsheng Yang
    • 1
  1. 1.National Key Laboratory of Crop Genetic Improvement, National Center of Oil Crop Improvement (Wuhan), College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanPeople’s Republic of China

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