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Mapping a major gene for growth habit and QTLs for ascochyta blight resistance and flowering time in a population between chickpea and Cicer reticulatum

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Abstract

Ascochyta blight is a devastating disease of chickpea. Breeders have been trying to introduce resistance from wild Cicer into cultivated chickpea, however, the effort is hampered by the frequent genetic drag of undesirable traits. Therefore, this study was aimed to identify potential markers linked to plant growth habit, ascochyta blight resistance and days to flowering for marker-assisted breeding. An interspecific F2 population between chickpea and C. reticulatum was constructed to develop a genetic linkage map. F2 plants were cloned through stem cuttings for replicated assessment of ascochyta blight resistance. A closely linked marker (TA34) on linkage group (LG) 3 was identified for plant growth habit explaining 95.2% of the variation. Three quantitative trait loci (QTLs) explaining approximately 49% of the phenotypic variation were found for ascochyta blight resistance on LG 3 and LG 4. Flowering time was controlled by two QTLs on LG3 explaining 90.2% of the variation. Ascochyta blight resistance was negatively correlated with flowering time (r = −0.22, P < 0.001) but not correlated with plant growth habit.

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Acknowledgements

We thank Dr Peter Winter and his group for providing sample microsatellite primers and technical advice. We are grateful to Professor Fred Muehlbauer, Dr Wallace Cowling, Professor Willie Erskine and Dr Judith Lichtenzveig for critical comments on the manuscript. We also thank Ms Leila Eshraghi for technical support and Miss Nola D’Souza and Professor Richard Oliver for providing some microsatellite primers. This project was supported by the Ministry of Science, Research and Technology of Iran.

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Authors and Affiliations

  1. School of Plant Biology (M084), Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia

    N. Aryamanesh, M. N. Nelson & G. Yan

  2. Centre for Legumes in Mediterranean Agriculture (CLIMA), The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia

    N. Aryamanesh, G. Yan, H. J. Clarke & K. H. M. Siddique

  3. Institute of Agriculture, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia

    N. Aryamanesh, M. N. Nelson, G. Yan, H. J. Clarke & K. H. M. Siddique

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  1. N. Aryamanesh
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  2. M. N. Nelson
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  3. G. Yan
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  4. H. J. Clarke
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  5. K. H. M. Siddique
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Correspondence to N. Aryamanesh.

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Aryamanesh, N., Nelson, M.N., Yan, G. et al. Mapping a major gene for growth habit and QTLs for ascochyta blight resistance and flowering time in a population between chickpea and Cicer reticulatum . Euphytica 173, 307–319 (2010). https://doi.org/10.1007/s10681-009-0086-2

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  • DOI: https://doi.org/10.1007/s10681-009-0086-2

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