Inheritance of seed phytate in mungbean (Vigna radiata (L.) Wilczek)
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Phytic acid (PA) is the main storage organic form of phosphorus (P) in seeds of cereals and legumes. It is a strong inhibitor against the absorption of nutrients in monogastric animals. The objective of this study was to determine the variation of total P (TP) in seeds of mungbean germplasm and investigate the inheritance of seed P compound and phytate contents. TP content in seeds of 250 accessions were found to range from 2.34 to 5.75 mg/g. The inheritance was studied in the F2 population derived from a cross between 2 accessions with the lowest and highest PA contents, viz. V1658BBR and V1141BG. Broad-sense heritability estimates of TP, inorganic P (IP), and phytate P (PhyP) contents were 80.8, 78.6 and 80.7%, respectively. The 9:7 segregation ratio of F2 population in this study indicated that high TP and PhyP were controlled by dominant alleles at 2 independent loci of major genes showing duplicated recessive epistasis.
KeywordsInositol phosphate Mungbean Phosphorus compounds Phytate Phytic acid Vigna radiata
This research was supported by Center for Agricultural Biotechnology of Kasetsart University, the Royal Golden Jubilee Scholarship of the Thailand Research Fund, and the Thailand’s National Science & Technology Development Agency.
- Alea GV, Noel MG (2007) Phytic acid in edible legume seeds. JRSCE 4(2):19–22Google Scholar
- Auesukaree C, Tochio H, Shirakawas M, Kaneko Y, Harashima S (2005) Plc1p, Agr82p, and Kcs1P, enzymes involved in inositol pyrophosphate synthesis, are essential for phosphate regulation and polyphosphate accumulation in Saccharomyces cerevisiae. J Biochem 280(26):25127–25133Google Scholar
- Coelho CMM, Santos JCP, Tsai SM, Vitorello VA (2002) Seed phytate content and phosphorus uptake and distribution in dry bean genotype. Braz J Plant 14(1):623–628Google Scholar
- Larson SR, Rutger JN, Young KA, Raboy V (2000) Isolation and genetic mapping of a non-lethal rice (Oryza sativa L.) low phytic acid 1 mutation. Crop Sci 40:1397–1405Google Scholar
- LeClerg EL, Leonard WH, Clark AG (1962) Field plot technique, 2nd edn. Burgess Publishing Co., MNGoogle Scholar
- Oltmans SE, Fehr WR, Welke GA, Cianzio SR (2004) Inheritance of low-phytate phosphorus in soybean. Crop Sci 44:433–435Google Scholar
- Pons WA Jr, Guthrie JD (1946) Determination of inorganic phosphorus in plant materials. J Ind Eng Chem 18(1):184–186Google Scholar
- R Development Core Team (2008) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN:3-900051-07-0. http://R-project.org
- Raboy V (2002) Progress in breeding low phytate crops. J Nutr 132(3):503–505Google Scholar
- Sompong U, Nakasathien S, Kaewprasit C, Srinives P (2007) Variation in phytic acid content in seed and correlation with agronomic traits of mungbean (Vigna radiata (L.) Wilczek). Agric Sci J 38:243–250 (in Thai with English abstract)Google Scholar