, Volume 171, Issue 3, pp 389–396 | Cite as

Inheritance of seed phytate in mungbean (Vigna radiata (L.) Wilczek)

  • Utumporn Sompong
  • Chongrak Kaewprasit
  • Sutkhet Nakasathien
  • Peerasak Srinives


Phytic acid (PA) is the main storage organic form of phosphorus (P) in seeds of cereals and legumes. It is a strong inhibitor against the absorption of nutrients in monogastric animals. The objective of this study was to determine the variation of total P (TP) in seeds of mungbean germplasm and investigate the inheritance of seed P compound and phytate contents. TP content in seeds of 250 accessions were found to range from 2.34 to 5.75 mg/g. The inheritance was studied in the F2 population derived from a cross between 2 accessions with the lowest and highest PA contents, viz. V1658BBR and V1141BG. Broad-sense heritability estimates of TP, inorganic P (IP), and phytate P (PhyP) contents were 80.8, 78.6 and 80.7%, respectively. The 9:7 segregation ratio of F2 population in this study indicated that high TP and PhyP were controlled by dominant alleles at 2 independent loci of major genes showing duplicated recessive epistasis.


Inositol phosphate Mungbean Phosphorus compounds Phytate Phytic acid Vigna radiata 



This research was supported by Center for Agricultural Biotechnology of Kasetsart University, the Royal Golden Jubilee Scholarship of the Thailand Research Fund, and the Thailand’s National Science & Technology Development Agency.


  1. Alea GV, Noel MG (2007) Phytic acid in edible legume seeds. JRSCE 4(2):19–22Google Scholar
  2. Auesukaree C, Tochio H, Shirakawas M, Kaneko Y, Harashima S (2005) Plc1p, Agr82p, and Kcs1P, enzymes involved in inositol pyrophosphate synthesis, are essential for phosphate regulation and polyphosphate accumulation in Saccharomyces cerevisiae. J Biochem 280(26):25127–25133Google Scholar
  3. Coelho CMM, Santos JCP, Tsai SM, Vitorello VA (2002) Seed phytate content and phosphorus uptake and distribution in dry bean genotype. Braz J Plant 14(1):623–628Google Scholar
  4. Dorsch JA, Cook A, Young KA, Anderson JM, Bauman AT, Volkmann CJ, Murthy PPN, Raboy V (2003) Seed phosphorus and inositol phosphate phenotype of barley low phytic acid genotypes. Phytochem 62:691–706CrossRefGoogle Scholar
  5. Dost K, Tokul O (2006) Determination of phytic acid in wheat and wheat products by reverse phase high performance liquid chromatography. Anal Chim Acta 558:22–27CrossRefGoogle Scholar
  6. Hitz WD, Carlson TJ, Kerr PS, Sebastian SA (2002) Biochemical and molecular characterization of a mutation that confers a decreased raffinosacharide and phytic acid phenotype on soybean seeds. Plant Physiol 128:650–660CrossRefPubMedGoogle Scholar
  7. Israel DW, Kwanyuen P, Burton JW (2006) Genetic variability for phytic acid phosphorus and inorganic phosphorus in seeds of soybean in maturity group V, VI and VII. Crop Sci 46:67–71CrossRefGoogle Scholar
  8. Larson SR, Rutger JN, Young KA, Raboy V (2000) Isolation and genetic mapping of a non-lethal rice (Oryza sativa L.) low phytic acid 1 mutation. Crop Sci 40:1397–1405Google Scholar
  9. LeClerg EL, Leonard WH, Clark AG (1962) Field plot technique, 2nd edn. Burgess Publishing Co., MNGoogle Scholar
  10. Lorenz A, Scott M, Lamkey KR (2008) Genetic variation and breeding potential of phytate and inorganic phosphorus in a maize population. Crop Sci 48:79–84CrossRefGoogle Scholar
  11. Mebrahtu T, Mohamed A, Elmi A (1997) Accumulation of phytate in vegetable soybean genotypes harvested at four developmental stages. Plant Food Hum Nutr 50:179–187CrossRefGoogle Scholar
  12. Ockenden I, Falk DE, Lott JNA (1997) Stability of phytate in barley and beans during storage. J Agric Food Chem 45:1673–1677CrossRefGoogle Scholar
  13. Ockenden I, Dorsch JA, Reid MM, Lin L, Grant LK, Raboy V, Lott JNA (2004) Characterization of the storage of phosphorus, inositol phosphate and cation in grain tissue of four barley (Hordeum vulgare L.) low phytic acid genotypes. Plant Sci 167:1131–1142CrossRefGoogle Scholar
  14. Oltmans SE, Fehr WR, Welke GA, Cianzio SR (2004) Inheritance of low-phytate phosphorus in soybean. Crop Sci 44:433–435Google Scholar
  15. Pilu R, Panzeri D, Gavazzi G, Rasmussen SK, Consoni G, Nielsen E (2003) Phenotypic, genetic and molecular characterization of a maize low phytic acid mutant (lpa241). Theor Appl Genet 107:980–987CrossRefPubMedGoogle Scholar
  16. Pilu R, Landoni M, Cassani E, Doria E, Nielsen E (2005) The maize lpa241 mutation causes a remarkable variability of expression and some pleiotropic effects. Crop Sci 45:2096–2105CrossRefGoogle Scholar
  17. Pons WA Jr, Guthrie JD (1946) Determination of inorganic phosphorus in plant materials. J Ind Eng Chem 18(1):184–186Google Scholar
  18. R Development Core Team (2008) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN:3-900051-07-0.
  19. Raboy V (2002) Progress in breeding low phytate crops. J Nutr 132(3):503–505Google Scholar
  20. Raboy V (2003) Molecules of interest: myo-inositol-1, 2, 3, 4, 5, 6-hexakisphosphate. Phytochem 64:1033–1043CrossRefGoogle Scholar
  21. Raboy V, Dickinson DB (1984) Effect of phosphorous and zinc nutrition on soybean seed phytic acid and zinc. Plant Physiol 75:1094–1098CrossRefPubMedGoogle Scholar
  22. Raboy V, Gerbasi PF, Young KA, Stoneberg SD, Pickett SG, Bauman AT, Murphy PPN, Sheridan WF, Ertl DS (2000) Origin and seed phenotype of maize low phytic acid 1-1 and low phytic acid 2-1. Plant Physiol 124:355–368CrossRefPubMedGoogle Scholar
  23. Raboy V, Young KA, Dorsch JA, Cook A (2001) Genetic and breeding of seed phosphorus and phytic acid. J Plant Physiol 158:489–497CrossRefGoogle Scholar
  24. Safrany ST, Cafrey JJ, Yang X, Shears SB (1999) Diphosphoinositol polyphosphates: the final frontier for inositide research? Biol Chem 380:945–951CrossRefPubMedGoogle Scholar
  25. Sandberg AS, Ahderinne R (1986) HPLC method for determination of inositol tri-, tetra-, penta- and hexaphosphates in foods and intestinal contents. J Food Sci 51(3):547–550CrossRefGoogle Scholar
  26. Sharpley AN, Charpa SC, Wedepohl R, Sims JY, Daniel TC, Reddy KR (1994) Managing agricultural phosphorus for protection of surface waters: issues and options. J Environ Qual 23:437–451CrossRefGoogle Scholar
  27. Shi J, Wang H, Wu Y, Hazebroek J, Meeley RB, Ertl DS (2003) The maize low-phytic acid mutant lpa2 is caused by mutation in an inositol phosphate kinase gene. Plant Physiol 131:507–515CrossRefPubMedGoogle Scholar
  28. Shi J, Wang H, Hazebroek J, Ertl DS, Harp T (2005) The maize low-phytic acid 3 encodes a myo-inositol kinase that plays a role in phytic acid biosynthesis in developing seeds. Plant J 42:708–719CrossRefPubMedGoogle Scholar
  29. Shi J, Wang H, Schellin K, Li B, Faller M, Stoop JM, Meeley RB, Ertl DS, Ranch JP, Glassman K (2007) Embryo-specific silencing of a transporter reduces phytic acid content of maize and soybean seeds. Nature Biotech 25:930–937CrossRefGoogle Scholar
  30. Sompong U, Nakasathien S, Kaewprasit C, Srinives P (2007) Variation in phytic acid content in seed and correlation with agronomic traits of mungbean (Vigna radiata (L.) Wilczek). Agric Sci J 38:243–250 (in Thai with English abstract)Google Scholar
  31. York JD, Odom AR, Murphy R, Ives EB, Wente SR (1999) A phospholipase C-dependent inositol polyphosphate kinase pathway required for efficient messenger RNA export. Science 285:96–100CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Utumporn Sompong
    • 1
    • 2
  • Chongrak Kaewprasit
    • 3
  • Sutkhet Nakasathien
    • 4
  • Peerasak Srinives
    • 1
  1. 1.Department of Agronomy, Faculty of Agriculture at Kamphaeng SaenKasetsart UniversityKamphaeng Saen, Nakhon PathomThailand
  2. 2.Center for Agricultural BiotechnologyKasetsart UniversityKamphaeng Saen, Nakhon PathomThailand
  3. 3.Science Program (Chemistry), Faculty of Liberal Arts and ScienceKasetsart UniversityKamphaeng Saen, Nakhon PathomThailand
  4. 4.Department of Agronomy, Faculty of AgricultureKasetsart UniversityBangkhen, BangkokThailand

Personalised recommendations