Skip to main content
SpringerLink
Log in

Molecular genetic analysis of flour color using a doubled haploid population in bread wheat (Triticum aestivum L.)

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Flour color is an important trait in the assessment of flour quality for the production of many end products. In this study, quantitative trait loci (QTLs) with additive effects, epistatic effects, and QTL × environment (QE) interactions for flour color in bread wheat (Triticum aestivum L.) were studied, using a set of 168 doubled haploid (DH) lines derived from a Huapei 3 × Yumai 57 cross. A genetic map was constructed using 283 simple sequence repeats (SSR) and 22 expressed sequence tags (EST)-SSR markers. The DH and parents were evaluated for flour color in three environments. QTL analyses were performed using QTLNetwork 2.0 software based on a mixed linear model approach. A total of 18 additive QTLs and 24 pairs of epistatic QTLs were detected for flour color, which were distributed on 19 of the 21 chromosomes. One major QTL, qa1B, closely linked to barc372 0.1 cM, could account for 25.64% of the phenotypic variation of a* without any influence from the environments. So qa1B could be used in the molecular marker-assisted selection (MAS) in wheat breeding programs. The results showed that both additive and epistatic effects were important genetic basis for flour color, and were also sometimes subject to environmental modifications. The information obtained in this study should be useful for manipulating the QTLs for flour color by MAS in wheat breeding programs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Börner A, Schumann E, Furste A, Coster H, Leithold B, Röder MS, Weber WE (2002) Mapping of quantitative trait loci determining agronomic important characters in hexaploid wheat (Triticum aestivum L.). Theor Appl Genet 105:921–936

    Article  PubMed  Google Scholar 

  • Cao G, Zhu J, He C, Gao Y, Yan J, Wu P (2001) Impact of epistasis and QTL×environment interaction on the developmental behavior of plant height in rice (Oryza sativa L.). Theor Appl Genet 103:153–160

    Article  CAS  Google Scholar 

  • Chen JF, Ren ZL, Gao LF, Jia JZ (2005) Developing new SSR markers from EST of wheat. Acta Agro Sini 31:154–158

    Google Scholar 

  • CIE (1976) CIE, Committee TC-1.3 CIE, Technical note. J Opt Soc Am 64:896–897

    Google Scholar 

  • Doerge RW (2002) Mapping and analysis of quantitative trait loci in experimental populations. Nat Rev 3:43–52

    CAS  Google Scholar 

  • Dudley JW, Lamkey KR, Geadelmann JL (1996) Evaluation of populations for their potential to improve three maize hybrids. Crop Sci 36:1553–1559

    Google Scholar 

  • Ellis MH, Rebetzke GJ, Azanza F, Richards RA, Spielmeyer W (2005) Molecular mapping of gibberellin responsive dwarfing genes in bread wheat. Theor Appl Genet 111:423–430

    Article  PubMed  CAS  Google Scholar 

  • Fan CC, Yu XQ, Xing YZ, Xu CG, Luo LJ, Zhang QF (2005) The main effects, epistatic effects and environmental interactions of QTLs on the cooking and eating quality of rice in a doubled-haploid line population. Theor Appl Genet 110:1445–1452

    Article  PubMed  CAS  Google Scholar 

  • Guo CQ, Bai ZA, Liao PA, Jin WK (2004) New high quality and yield wheat variety Yumai 57. China Seed Ind 4:54

    Google Scholar 

  • Hai Y, Kang MH (2007) Breeding of Hupei 3 new wheat variety with high yield and early maturing. Henan Agric Sci 5:36–37

    Google Scholar 

  • Huang XQ, Coster H, Ganal MW, Röder MS (2003) Advanced backcross QTL analysis for the identification of quantitative trait loci alleles from wild relatives of wheat (Triticum aestivum L.). Theor Appl Genet 106:1379–1389

    PubMed  CAS  Google Scholar 

  • Huang XQ, Cloutier S, Lycar L, Radovanovic N, Humphreys DG, Noll JS, Somers DJ, Brown PD (2006) Molecular detection of QTLs for agronomic and quality traits in a doubled haploid population derived from two Canadian wheats (Triticum aestivum L.). Theor Appl Genet 113:753–766

    Article  PubMed  CAS  Google Scholar 

  • Hutchings JB (1994) Food color and appearance. Chapman and Hall, Great Britain; Blackie Academic and Professional, London

  • Karakousis A, Gustafson JP, Chalmers KJ, Barr AR, Langridge P (2003) A consensus map of barley integrating SSR, RFLP, and AFLP markers. Aust J Agric Res 54:1173–1185

    Article  CAS  Google Scholar 

  • Knott DR (1984) The genetic nature of mutations of a gene for yellow pigment linked to Lr19 in ‘Agatha’ wheat. Can J Genet Cytol 26:392–393

    Google Scholar 

  • Kruger JE, Anderson MH, Dexter JE (1994) Effect of flour refinement on raw cantonese noodle color and texture. Cereal Chem 71:177–182

    CAS  Google Scholar 

  • Li X, Yang W, Li Y, Liu D, Yan H, Meng Q, Zhang T (2005) A SSR marker for leaf rust resistance gene Lr19 in wheat. Sci Agric Sini 38:1156–1159

    CAS  Google Scholar 

  • Lin HX, Yamamoto T, Sasaki T, Yano M (2000) Characterization and detection of epistatic interactions of three QTLs, Hd1, Hd2 and Hd3, controlling heading date in rice using nearly isogenic lines. Theor Appl Genet 101:1021–1028

    Article  CAS  Google Scholar 

  • Liu DC, Gao MQ, Guan RX, Li RZ, Cao SH, Guo XL, Zhang AM (2002) Mapping Quantitative trait loci for plant height in wheat (Triticum aestivum L.) using a F2:3 population. Acta Genet Sini 9:706–711

    Article  Google Scholar 

  • Liu GF, Yang J, Xu HM, Zhu J (2007a) Influence of epistasis and QTL × environment interaction on heading date of rice (Oryza sativa L.). J Genet Genomics 34:608–615

    Article  PubMed  Google Scholar 

  • Liu Y, Yan H, Yang W, Meng Q, Zhang T, Liu D (2007b) Identification of a SRAP markers linked to leaf rust resistance gene Lr19 in wheat. Acta Agric Boreali Sini 22:193–196

    Google Scholar 

  • Ma JF, Shen RF, Zhao ZQ, Wissuwa M, Takeuchi Y, Ebitani T, Yano M (2002) Response of rice to Al stress and identification of quantitative trait loci for Al tolerance. Plant Cell Physiol 43:652–659

    Article  PubMed  CAS  Google Scholar 

  • Ma W, Appels R, Bekes F, Larroque O, Morell MK, Gale KR (2005) Genetic characterisation of dough rheological properties in a wheat doubled haploid population: additive genetic effects and epistatic interactions. Theor Appl Genet 111:410–422

    Article  PubMed  CAS  Google Scholar 

  • Ma XQ, Tang JH, Teng WT, Yan JB, Meng YJ, Li JS (2007) Epistatic interaction is an important genetic basis of grain yield and its components in maize. Mol Breed 20:41–51

    Article  Google Scholar 

  • Mares DJ, Campbell AW (2001) Mapping components of flour and noodle color in Australian wheat. Aust J Agric Res 52:1297–1309

    Article  CAS  Google Scholar 

  • Miskelly DM (1984) Flour components affecting paste and noodle color. J Sci Food Agric 35:463–471

    Article  Google Scholar 

  • Parker GD, Langridge P (2000) Development of a STS marker linked to a major locus controlling flour color in wheat (Triticum aestivum L.). Mol Breed 6:169–174

    Article  CAS  Google Scholar 

  • Parker GD, Chalmers KJ, Rathjen AJ, Langridge P (1998) Mapping loci associated with flour color in wheat (Triticum aestivum L.). Theor Appl Genet 97:238–245

    Article  CAS  Google Scholar 

  • Pestsova E, Ganal MW, Röder MS (2000) Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome 43:689–697

    Article  PubMed  CAS  Google Scholar 

  • Rebetzke GJ, Ellis MH, Bonnett DG, Richards RA (2007) Molecular mapping of genes for Coleoptile growth in bread wheat (Triticum aestivum L.). Theor Appl Genet 114:1173–1183

    Article  PubMed  CAS  Google Scholar 

  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023

    PubMed  Google Scholar 

  • Saito K, Hayano-Saito Y, Maruyama-Funatsuki W, Sato Y, Kato A (2004) Physical mapping and putative candidate gene identification of a quantitative trait locus Ctb1 for cold tolerance at the booting stage of rice. Theor Appl Genet 109:515–522

    Article  PubMed  CAS  Google Scholar 

  • Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114

    Article  PubMed  CAS  Google Scholar 

  • Sun XD, Wang LK, Ren HB, Lan J (2002) The application of tristimulus colorimeter in the determination of flour color. Technol Oil Food 10:31–33

    Google Scholar 

  • Wang DL, Zhu J, Li ZK, Paterson AH (1999) Mapping QTLs with epistatic effects and QTL×environment interactions by mixed linear model approaches. Theor Appl Genet 99:1255–1264

    Article  Google Scholar 

  • Yang J, Zhu J (2005) Predicting superior genotypes in multiple environments based on QTL effects. Theor Appl Genet 110:1268–1274

    Article  PubMed  Google Scholar 

  • Zhang X, Tian JC (2008) The color advantage of Chinese wheat with high whiteness and analysis of factors affecting color formation. Sci Agri Sini 41:347–353

    CAS  Google Scholar 

  • Zhang LP, Yan J, Xia XC, He ZH, Sutherland MW (2006) QTL mapping for kernel yellow pigment content in common wheat Mapping QTLs for polyphenol oxidase activity in a DH population from common wheat. Acta Agro Sini 32:41–45

    Google Scholar 

Download references

Acknowledgements

The authors gratefully thank Professor Yan Hai (Henan Academy of Agricultural Sciences, Zhengzhou, China) for kindly providing the research materials and Dr. Xianchun Xia (Chinese Academy of Agricultural Sciences, Beijing, China) for donating some primers. The present research was supported by the National Natural Science Foundation of China (30671270), Hi-Tech Research and Development (863) Program of China (2006AA10Z1E9 and 2006AA100101), and Improved Variety Project of Shandong Province (LN2006-6).

Author information

Authors and Affiliations

  1. State Key Laboratory of Crop Biology, Group of Quality Wheat Breeding of Shandong Agricultural University, No. 61 Daizong Road, Tai’an, 271018, China

    Kun-Pu Zhang, Liang Zhao, Bin Liu & Ji-Chun Tian

  2. Agronomic Department, Dezhou College, Dezhou, 253500, China

    Guang-Feng Chen

  3. Dezhou Academy of Agricultural Sciences, Dezhou, 253500, China

    Xian-Bin Xu

Authors
  1. Kun-Pu Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Guang-Feng Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Liang Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bin Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xian-Bin Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ji-Chun Tian
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ji-Chun Tian.

Additional information

Kun-Pu Zhang and Guang-Feng Chen contributed equally to this study.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, KP., Chen, GF., Zhao, L. et al. Molecular genetic analysis of flour color using a doubled haploid population in bread wheat (Triticum aestivum L.). Euphytica 165, 471–484 (2009). https://doi.org/10.1007/s10681-008-9756-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-008-9756-8

Keywords

Search

Navigation