, Volume 162, Issue 1, pp 117–128 | Cite as

Development of a complete set of disomic rape-radish chromosome-addition lines

  • H. Budahn
  • O. Schrader
  • H. Peterka


After crosses of multiple rape-radish chromosome additions with rape, nine different monosomic additions (2n = 4x = 38 + 1) of individual radish chromosomes, a–i, were isolated, having the genomic background of winter oil seed rape and radish cytoplasm. Extra chromosomes were identified with RAPD markers, specific for individual radish chromosomes. All radish-plasmic monosomic additions, except that of chromosome f, had low seed production due to pistilloid stamens. Rape cytoplasm was substituted by crossing the monosomic additions as pollinators to rape followed by reselection of the radish chromosome additions. The monosomic additions in rape cytoplasm showed normal male fertility. Average transmission rates of radish chromosomes via egg cells and pollen cells were 37% and 27%, respectively. Lowest male transmission was found for chromosomes e and f with 0.04 and 0.01. Monosomic additions were self-pollinated to produce disomic additions. Monosomic and disomic addition progenies were discriminated by a prescreening with quantitative double primer (dp) RAPDs and cytological confirmation of preselected candidates by FISH analysis using a Raphanus-specific probe. Usability of this complete set of nine disomic rape-radish additions (2n = 4x = 38 + 2) was discussed.


Brassica napus Raphanus sativus FISH Chromosome-specific marker Interspecific hybridization Monosomic alien addition line 



The authors are grateful to V. Ripley for critical reading the manuscript. They thank A. Garve, U. Höfling and K. Meyer for their excellent technical assistance.


  1. Ananiev EV, Phillips RL, Rines HW (1998) Chromosome-specific molecular organization of maize (Zea mays L.) centromeric regions. Proc Natl Acad Sci USA 95:13073–13078PubMedCrossRefGoogle Scholar
  2. Bannerot H, Boulidard L, Cauderon Y, Tempe J (1974) Transfer of cytoplasmic male sterility from Raphanus sativus to Brassica oleracea. Proc EUCARPIA Meet Crop Sect Cruciferae 25:52–54Google Scholar
  3. Bannerot H, Boulidard L, Chupeau Y (1977) Unexpected difficulties met with the radish cytoplasm in Brassica oleracea. Eucarpia Cruciferae Newlett 2:16Google Scholar
  4. Baukloh H (1976) Untersuchungen zur Wirtspflanzeneignung der Kruziferen gegenüber dem Rübennematoden Heterodera schachtii (Schmidt), unter besonderer Berücksichtigung der Resistenzzüchtung. PhD thesis, Georg-August-Universität, GöttingenGoogle Scholar
  5. Bett KE, Lydiate DJ (2003) Genetic analysis and genome mapping in Raphanus. Genome 46:423–430PubMedCrossRefGoogle Scholar
  6. Biyashev RM, Ragab RA, Maughan PJ, Saghai-Maroof MA (1997) Molecular mapping, chromosomal assignment, and genetic diversity analysis of phytochrome loci in barley (Hordeum vulgare). J Hered 88:21–26Google Scholar
  7. Budar F, Pelletier G (2001) Male sterility in plants: occurrence, determinism, significance and use. CR Acad Sci Paris Sér III 324:543–550Google Scholar
  8. Chen BY, Simonsen V, Lanner-Herrera C, Heneen WK (1992) A Brassica campestris-alboglabra addition line and its use for gene mapping, intergenomic gene transfer and the generation of trisomics. Theor Appl Genet 84:592–599Google Scholar
  9. Cheng BF, Chen BY, Heneen WK (1994) Addition of Brassica alboglabra Bailey chromosomes to B. campestris L. with special emphasis on seed color. Heredity 73:185–189CrossRefGoogle Scholar
  10. Chetelat RT, Rick CM, Cisneros P, Alpert KB, DeVerna JW (1998) Identification, transmission, and cytological behaviour of Solanum lycopersicoides Dun. Monosomic alien addition lines in tomato (Lycopersicon esculentum Mill.). Genome 41:40–50CrossRefGoogle Scholar
  11. Chevre AM, Eber F, Barret P, Dupuy P, Brace J (1997) Identification of the different Brassica nigra chromosomes from both sets of B. oleracea–B. nigra and B. napus–B. nigra addition lines with a special emphasis on chromosome transmission and self-incompatibility. Theor Appl Genet 94:603–611CrossRefGoogle Scholar
  12. Cho S, Garvin DF, Muehlbauer GJ (2006) Transcriptome analysis and physical mapping of barley genes in wheat–barley chromosome addition lines. Genetics 172:1277–1285PubMedCrossRefGoogle Scholar
  13. Delourme R, Foisset N, Horais R, Barret P, Champagne G, Cheung WY, Landry BS, Renard M (1998) Characterization of the radish introgression carrying the Rfo restorer gene for the Ogu-INRA cytoplasmic male sterility in rapeseed (Brassica napus L.) Theor Appl Genet 97:129–134CrossRefGoogle Scholar
  14. Fantes JA, MacKay GR (1979) The production of disomic addition lines of Brassica campestris. Cruciferae Newslett 4:36–37Google Scholar
  15. Gao D, Guo D, Jung C (2001) Monosomic addition lines of Beta corolliflora Zoss in sugar beet: cytological and molecular-marker analysis. Theor Appl Genet 103:240–247CrossRefGoogle Scholar
  16. Gao D, Jung C (2002) Monosomic addition lines of Beta corolliflora in sugar beet: plant morphology and leaf spot resistance. Plant Breed 121:81–86CrossRefGoogle Scholar
  17. Garriga-Calderé F, Huigen DJ, Filotico F, Jacobsen E, Ramanna MS (1997) Identification of alien chromosomes through GISH and RFLP analysis and the potential for establishing potato lines with monosomic additions of tomato chromosomes. Genome 40:666–673PubMedCrossRefGoogle Scholar
  18. Hernandez P, Rubio MJ, Martin A (1995) Development of RAPD markers in tritordeum and addition lines of Hordeum chilense and Triticum aestivum. Plant Breed 115:52–56CrossRefGoogle Scholar
  19. Heyn FW (1976) Transfer of restorer genes from Raphanus to cytoplasmic male sterile Brassica napus. Eucarpia Cruciferae Newslett 1:15–16Google Scholar
  20. Hirai K, Irifube K, Tanaka R, Morikawa H (1995) Molecular and cytological characterization of a highly repeated DNA sequence in Raphanus sativus. Genome 38:1237–1243PubMedGoogle Scholar
  21. Jahier J, Chevre AM, Tanguy AM, Eber F (1989) Extraction of disomic addition lines Brassica napus-B. nigra. Genome 32:408–413Google Scholar
  22. Jena KK, Khush GS (1989) Monosomic alien addition of rice—production, morphology, cytology, and breeding behaviour. Genome 32:449–455 Google Scholar
  23. Jung C, Koch R, Fischer F, Brandes A, Wricke G, Herrmann RG (1992) DNA markers closely linked to nematode resistance genes in sugar beet (Beta vulgaris L) mapped using chromosome additions and translocations originating from wild beets of the Procumbentes section. Mol Gen Genet 232:271–278PubMedGoogle Scholar
  24. Kaneko Y, Matsuzawa Y, Sarashima M (1987) Breeding of the chromosome addition lines of radish with single kale chromosome. Jpn J Breed 37:438–452Google Scholar
  25. Kaneko Y, Yano H, Bang SW, Matsuzawa Y (2001) Production and characterization of Raphanus sativus-Brassica rapa monosomic chromosome addition lines. Plant Breed 120:163–168CrossRefGoogle Scholar
  26. Karpechenko GD (1924) Hybrids of Raphanus sativus L. × Brassica oleracea L J Genet 14:375–396CrossRefGoogle Scholar
  27. Krämer R, Scholze P, Marthe F, Ryschka U, Klocke E, Schumann G (2003) Improvement of disease resistance in cabbage: 1. Turnip mosaic virus, TuMV. Gesunde Pflanzen 55:193–198CrossRefGoogle Scholar
  28. Kynast RG, Okagaki RJ, Galatowitsch MW, Granath SR, Jacobs MS, Stec AO, Rines HW, Phillips RL (2004) Dissecting the maize genome by using chromosome addition and radiation hybrid lines. Proc Natl Acad Sci USA 101:9921–9926PubMedCrossRefGoogle Scholar
  29. Leino M, Teixeira R, Landgren M, Glimelius K (2003) Brassica napus lines with rearranged Arabidopsis mitochondria display CMS and a range of developmental aberrations. Theor Appl Genet 106:1156–1163PubMedGoogle Scholar
  30. Li LJ, Arumuganathan K, HW Rines RL Phillips, Riera-Lizarazu O, Sandhu D, Zhou Y, Gill KS (2001) Flow cytometric sorting of maize chromosome 9 from an oat–maize chromosome addition line. Theor Appl Genet 102:658–663CrossRefGoogle Scholar
  31. Lubberts JH, Toxopeus H (1982) The development of beet cyst nematode-resistant fodder rape and white mustard (in Dutch). Zaadbelangen 36:66–69Google Scholar
  32. McGrath JM, Quiros CF (1990) Generation of alien chromosome addition lines from synthetic Brassica napus: morphology, cytology, fertility and chromosome transmission. Genome 33:374–383Google Scholar
  33. Multani DS, Jena KK, Brar DS, Reyes de los BG, Angeles ER, Khush GS (1994) Development of monosomic alien addition lines and introgression of genes from Oryza australiensis Domin. to cultivated rice, O. sativa. Theor Appl Genet 88:102–109CrossRefGoogle Scholar
  34. Peffley EB, Corgan JN ,Horak KE, Tanksley SD (1985) Electrophoretic analysis of Allium alien addition lines. Theor Appl Genet 71:176–184Google Scholar
  35. Peterka H, Budahn H, Schrader O, Ahne R, Schütze W (2004) Transfer of resistance against the beet cyst nematode from radish (Raphanus sativus) to rape (Brassica napus) by monosomic chromosome addition. Theor App Genet 109:30–41CrossRefGoogle Scholar
  36. Quiros CF, Ochoa O, Kianian SF, Douches D (1987) Analysis of the Brassica oleracea genome by the generation of B. campestris–oleracea chromosome addition lines: characterization by isozymes and rDNA genes. Theor Appl Genet 74:758–766Google Scholar
  37. Quiros CF, Ochoa O, Hosaka Y, Kianian SK , McGrath M, Oard M, This P, Delseny M, Truco MJ, Perez I, Arus P (1988) Alien addition lines in Brassicas—construction, characterization and their possible applications. Hortscience 23:777Google Scholar
  38. Riera-Lizarazu O, Vales MI, Ananiev EV, Rines HW, Phillips RL (2000) Production and characterization of maize chromosome 9 radiation hybrids derived from an oat–maize addition line. Genetics 156:327–339PubMedGoogle Scholar
  39. Rod J (1993) Assessment of Raphanus sativus resistance to clubroot (Plasmodiophora brassicae). Ann Appl Biol 122:124–125Google Scholar
  40. Schmidt T, Junghans H, Metzlaff M (1990) Construction of Beta procumbens-specific DNA probes and their application for the screening of Beta vulgaris × Beta procumbens (2n = 19) addition lines. Theor Appl Genet 79:177–181CrossRefGoogle Scholar
  41. Silue D, Nashaat NI, Tirilly Y (1996) Differential responses of Brassica oleracea and B. rapa accessions to seven isolates of Peronospora parasitica at the cotyledon stage. Plant Disease 80:142–144CrossRefGoogle Scholar
  42. Singh RJ, Kollipara KP, Hymowitz Th (1998) Monosomic alien addition lines derived from Glycine max (L.) Merr. and G. tomentella Hayata: production, Characterization, and Breeding Behavior. Crop Sci 38:1483–1489CrossRefGoogle Scholar
  43. Struss D, Bellin U, Röbbelen G (1991) Development of B-genome chromosome addition lines of B. napus using different interspecific Brassica hybrids. Plant Breed 106:209–214CrossRefGoogle Scholar
  44. Suen DF, Wang CK ,Lin RF, Kao YY, Lee FM, Chen CC (1997) Assignment of DNA markers to Nicotiana sylvestris chromosomes using monosomic alien addition lines. Theor Appl Genet 94:331–337CrossRefGoogle Scholar
  45. This P, Ochoa O, Quiros CF (1990) Dissection of the Brassica nigragenome by monosomic addition lines. Plant Breed 105:211–220CrossRefGoogle Scholar
  46. Tsuro M, Suwabe K, Kubo N, Matsumoto S, Hirai M (2005) Construction of a molecular linkage map of radish (Raphanus sativus L.), based on AFLP and Brassica-SSR markers. Breed Sci 55:107–111CrossRefGoogle Scholar
  47. Welsh J, McClelland M (1991) Genomic fingerprinting with AP-PCR using pairwise combination of primers: application to genetic mapping of the mouse. Nucleic Acids Res 19:5275–5279PubMedCrossRefGoogle Scholar
  48. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535PubMedCrossRefGoogle Scholar
  49. Yang YW, Tai PY, Chen Y, Li WH (2002) A study of the phylogeny of Brassica rapa, B. nigra, Raphanus sativus, and their related genera using noncoding regions of chloroplast DNA. Mol Phylogenet Evol 23:268–275 PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  1. 1.Federal Centre for Breeding Research on Cultivated PlantsInstitute of Horticultural CropsQuedlinburgGermany

Personalised recommendations