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Euphytica

, Volume 162, Issue 1, pp 117–128 | Cite as

Development of a complete set of disomic rape-radish chromosome-addition lines

  • H. Budahn
  • O. Schrader
  • H. Peterka
Article

Abstract

After crosses of multiple rape-radish chromosome additions with rape, nine different monosomic additions (2n = 4x = 38 + 1) of individual radish chromosomes, a–i, were isolated, having the genomic background of winter oil seed rape and radish cytoplasm. Extra chromosomes were identified with RAPD markers, specific for individual radish chromosomes. All radish-plasmic monosomic additions, except that of chromosome f, had low seed production due to pistilloid stamens. Rape cytoplasm was substituted by crossing the monosomic additions as pollinators to rape followed by reselection of the radish chromosome additions. The monosomic additions in rape cytoplasm showed normal male fertility. Average transmission rates of radish chromosomes via egg cells and pollen cells were 37% and 27%, respectively. Lowest male transmission was found for chromosomes e and f with 0.04 and 0.01. Monosomic additions were self-pollinated to produce disomic additions. Monosomic and disomic addition progenies were discriminated by a prescreening with quantitative double primer (dp) RAPDs and cytological confirmation of preselected candidates by FISH analysis using a Raphanus-specific probe. Usability of this complete set of nine disomic rape-radish additions (2n = 4x = 38 + 2) was discussed.

Keywords

Brassica napus Raphanus sativus FISH Chromosome-specific marker Interspecific hybridization Monosomic alien addition line 

Notes

Acknowledgments

The authors are grateful to V. Ripley for critical reading the manuscript. They thank A. Garve, U. Höfling and K. Meyer for their excellent technical assistance.

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  1. 1.Federal Centre for Breeding Research on Cultivated PlantsInstitute of Horticultural CropsQuedlinburgGermany

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