A new male sterile mutant LZ in wheat (Triticum aestivum L.)
- 296 Downloads
Genetic male sterility (GMS) genes in wheat (Triticum aestivum L.) can be used for commercial hybrid seed production. A new wheat GMS mutant, LZ, was successfully used in the 4E-ms system for producing hybrid wheat, a new approach of producing hybrid seed based on GMS. Our objective was to analyse the genetic mechanism of male sterility and locate the GMS gene in mutant LZ to a chromosome. We firstly crossed male sterile line 257A (2n = 42) derived from mutant LZ to Chinese Spring and several other cultivars for determining the self-fertility of the F1 hybrids and the segregation ratios of male-sterile and fertile plants in the F2 and BC1 generations. Secondly, we conducted nullisomic analysis by crossing male sterile plants of line 257A to 21 self-fertile nullisomic lines as male to test the F1 fertilities and to locate the GMS gene in mutant LZ to a chromosome. Thirdly, we conducted an allelism test with Cornerstone, which has ms1c located on chromosome 4BS. All F1s were male fertile and the segregation ratio of male-sterile: fertile plants in all BC1 and F2 populations fitted 1:1 and 1:3 ratios, respectively. The male sterility was stably inherited, and was not affected by environmental factors in two different locations or by the cytoplasm of wheat cultivars in four reciprocal cross combinations. The results of nullisomic analysis indicated the gene was on chromosome 4B. The allelism test showed that the mutant LZ was allelic to ms1c. We concluded that the mutant LZ has common wheat cytoplasm and carries a stably inherited monogenic recessive gene named ms1g.
KeywordsWheat Genetic male sterility Genetic analysis Nullisomic analysis Allelism
This research was supported by the National Natural Science Fund (30170593).
- Driscoll CJ (1981) New approaches to wheat breeding. In: Evens LT, Peacock WJ (eds) Wheat science today and tomorrow. Cambridge University Press, Great Britain, pp 97–106Google Scholar
- Deng JY, Gao ZL (1980) Discovery and identification of a dominant male sterile gene in wheat. Acta Agron Sin 6(2):84–98 (in Chinese) Google Scholar
- Fossati A, Ingold M (1970) A male sterile mutant in Triticum aestivum. Wheat Info Serv 30:8–10Google Scholar
- Kaul MLH (1988) Male sterility in higher plants. Springer-Verlag, Berlin & HeidelbergGoogle Scholar
- Liu BH, Deng JY (1986) The chromosome localization and telomere analyses of a dominant male sterility gene in wheat. Chinese Sci 2:157–165 (in Chinese)Google Scholar
- Liu BH, Yang L (1991) Breeding of dwarfing-sterile wheat and its potential values in wheat breeding. Chin Sci Bull 36(4):306–308 (in Chinese)Google Scholar
- Maan SS, Kianian SF (2001) Third dominant male sterility gene in common wheat. Wheat Info Serv 93:27–31Google Scholar
- McIntosh RA, Hart GE, Devos KM, Gale MD, RogersWJ (1998). Catalogue of gene symbols for wheat. In: AE Slinkard (ed) Proc 9th Int Wheat Genet Symp, vol 5. University of Saskatchewan Extension Press, Saskatoon, Canada, 1998Google Scholar
- Sears ER (1954) The aneuploids of common wheat. Missouri Agric Exp Sta Res Bull 572:1–58Google Scholar
- Xue XZ, Ji WQ, Wang QY, Xu XT (1991) Establishment of stable self-fertile nullisomic lines of the wheat cultivar Abbondanza. Acta Agron Sin 17(6):417–423 (in Chinese) Google Scholar
- Xue XZ, Ji WQ, Wang QY, Zhao HX, Chen JL (1995) Nullisomic analysis of wheat characteristics. Acta Agri Boreali-Occidentalis Sin 4(4):1–5 (in Chinese)Google Scholar