, Volume 159, Issue 3, pp 333–341 | Cite as

Mapping of a gene (Vir) for a non-glaucous, viridescent phenotype in bread wheat derived from Triticum dicoccoides, and its association with yield variation

  • J. R. Simmonds
  • L. J. Fish
  • M. A. Leverington-Waite
  • Y. Wang
  • P. Howell
  • J. W. Snape


The introgression of desirable genes or alleles from the wild relatives of hexaploid wheat can be a valuable source of genetic variation for wheat breeders to enhance modern varieties. The UK Group 1 bread making variety Shamrock is an example where the introgression of genetic material from wild emmer (Triticum dicoccoides) has been used to develop a modern cultivar. A striking character of Shamrock is its unique viridescent colour compared to other UK wheats, a trait that coincides with a non-glaucous phenotype. A doubled haploid population segregating for the trait (Shamrock × Shango) was examined to map the location of Vir, and analyse any associated pleiotropic effects. The viridescence gene located to the distal end of the short arm of chromosome 2B. QTL analysis of productivity traits shows an association between Vir and a significant delay in senescence, resulting in an extension of the grain filling period. A stable yield QTL, accounting for up to a quarter of the variation in one case, was also identified at or near Vir, indicating significant yield benefits either by linkage or pleiotropy.


Glaucosity Stay-green Triticum dicoccoides Viridescence Wheat Yield 



We would like to thank the Department for Environment, Food and Rural Affairs (DEFRA) and the Biotechnology and Biological Sciences Research Council (BBSRC) for their financial assistance through the LINK project “Investigating Wheat Functionality through Breeding and End-use” (FQS23). We are also grateful to Syngenta Crop Protection UK Limited and RAGT Seeds Ltd for their contributions to the phenotype data for this study. JIC is sponsored by the UK Biotechnology and Biological Sciences Research Council.


  1. Bassam B, Caetano Anolles G, Gresshoff P (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal Biochem 196:80–83PubMedCrossRefGoogle Scholar
  2. Carver BF, Johnson RC, Rayburn AL (1989) Genetic analysis of photosynthetic variation in hexaploid and tetraploid wheat and their interspecific hybrids. Photosynth Res 20:105–118Google Scholar
  3. Driscoll CJ (1966) Gene-centromere distances in wheat by aneuploid F2 observations. Genetics 54:131–135PubMedGoogle Scholar
  4. Evans LT (1993) Crop evolution, adaptation and yield. Cambridge University Press, Cambridge, UKGoogle Scholar
  5. Evans LT, Dunstone RL (1970) Some physiological aspects of evolution in wheat. Aust J Biol Sci 26:295–307Google Scholar
  6. Fahima T, Röder M, Grama A, Nevo E (1998) Microsatellite DNA polymorphism divergence in Triticum dicoccoides accessions highly resistant to yellow rust. Theor Appl Genet 96:187–195CrossRefGoogle Scholar
  7. Garcia del Moral LF, Rharrabti Y, Villegas D, Royo C (2003) Evaluation of grain yield and its components in durum wheat under Mediterranean conditions. Agron J 95:266–274CrossRefGoogle Scholar
  8. Gerechter-Amitai ZK, Stubbs RW (1970) A valuable source of yellow rust resistance in Israeli populations of wild emmer, Triticum dicoccoides Koren. Euphytica 19:12–21CrossRefGoogle Scholar
  9. Grama A, Gerechter-Amitai ZK, Blum A (1983) Wild emmer as a donor of genes for resistance to stripe rust and for high protein content. In: Sakamoto S (ed) Proc 6th Int Wheat Genet Symp, Kyoto, Japan: Plant Germplasm Institute, University of Kyoto, 187–192Google Scholar
  10. Jaccoud D, Peng K, Feinstein D, Kilian A (2001) Diversity arrays: a solid state technology for sequence information independent genotyping. Nucl Acids Res 29(4):e25PubMedCrossRefGoogle Scholar
  11. Jenson NF, Driscoll CJ (1962) Inheritance of the waxless character in wheat. Crop Sci 2:504–505CrossRefGoogle Scholar
  12. Kearsey MJ, Hyne V (1994) QTL analysis a simple ‘marker regression’ approach. Theor Appl Genet 89:698–702CrossRefGoogle Scholar
  13. Kushnir U, Halloran GM (1984) Transfer of high kernel weight and high protein from wild tetraploid wheat (Triticum turgidum/dicoccoides) to bread wheat (T. aestivum) using homologous and homoeologous recombination. Euphytica 33:249–255CrossRefGoogle Scholar
  14. Laurie DA, Reymondie S (1991) High frequencies of fertilization and haploid seedling production in crosses between commercial hexaploid wheat varieties and maize. Plant Breed 106:182–189CrossRefGoogle Scholar
  15. Monneveux P, Reynolds MP, Gonzalez-Santoyo H, Pena RJ, Mayr L, Zapata F (2004) Relationships between grain yield, flag leaf morphology, carbon isotope discrimination and ash content in irrigated wheat. J Agron Crop Sci 190:395–401CrossRefGoogle Scholar
  16. Nevo E, Gerechter-Amitai ZK, Beiles A (1991) Resistance of wild emmer wheat to stem rust: ecological, pathological and allozyme associations. Euphytica 53:121–130CrossRefGoogle Scholar
  17. Nevo E, Gerechter-Amitai ZK, Beiles A, Golenberg EM (1986) Resistance of wild wheat to stripe rust: predictive method by ecology and allozyme genotypes. Plant Syst Evol 153:13–30CrossRefGoogle Scholar
  18. Nevo E, Moseman JG, Beiles A, Zohary D (1985) Patterns of resistance of Israeli wild emmer wheat to pathogens. I. Predictive method by ecology and allozyme genotypes for powdery mildew and leaf rust. Genetica 67:209–222CrossRefGoogle Scholar
  19. NIAB (2002) Pocket guide to varieties of cereals, oilseeds and pulses—Autumn 2002. Cambridge Marketing LimitedGoogle Scholar
  20. Peng J, Korol AB, Fahima T, Roder MS, Ronin YI, Li YC, Nevo E (2000) Molecular genetic maps in wild emmer wheat, Triticum dicoccoides: genome-wide coverage, massive negative interference, and putative quasi-linkage. Genome Res 10:1509–1531PubMedCrossRefGoogle Scholar
  21. Richards RA, Rawson HM, Johnson DA (1986) Glaucousness in wheat: its development and effect on water-use efficiency, gas exchange and photosynthetic tissue temperatures. Aust J Plant Physiol 13:465–473Google Scholar
  22. Snape JW, Foulkes JM, Simmonds J, Leverington M, Fish LJ, Wang Y, Ciavarrella M (2007) Dissecting gene × environmental effects on wheat yields via QTL and physiological analysis. Euphytica 154:401–408CrossRefGoogle Scholar
  23. Somers DJ, Isaac P, Edwards K (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114PubMedCrossRefGoogle Scholar
  24. Thomas H, Howarth CJ (2000) Five ways to stay green. J Exp Biol 51:329–337Google Scholar
  25. Tsunewaki K, Ebana K (1999) Production of near-isogenic lines of common wheat for glaucousness and genetic basis of this trait clarified by their use. Genes Genet Syst 74:33–41CrossRefGoogle Scholar
  26. Verma V, Foulkes MJ, Worland AJ, Sylvester-Bradley R, Caligari PDS, Snape JW (2004) Mapping quantitative trait loci for flag leaf senescence as a yield determinant in winter wheat under optimal and drought-stressed environments. Euphytica 135:255–263CrossRefGoogle Scholar
  27. Wenzl P, Carling J, Kudrna D, Jaccoud D, Huttner E, Kleinhofs A, Kilian A (2004) Diversity Arrays Technology (DArT) for whole genome profiling of barley. Proc Natl Acad Sci USA 101:9915–9920PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • J. R. Simmonds
    • 1
  • L. J. Fish
    • 1
  • M. A. Leverington-Waite
    • 1
  • Y. Wang
    • 1
  • P. Howell
    • 2
  • J. W. Snape
    • 1
  1. 1.Crop Genetics DepartmentJohn Innes CentreNorwichUK
  2. 2.Syngenta Crop Protection UK LimitedCambridgeUK

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