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Euphytica

, Volume 159, Issue 1–2, pp 233–239 | Cite as

RAPD markers linked to a rust resistance gene in cultivated groundnut (Arachis hypogaea L.)

  • Suvendu Mondal
  • Anand M. Badigannavar
  • G. S. S. Murty
Article

Abstract

Groundnut rust (Puccinia arachidis Speg.) is an important air borne pathogen, which causes substantial losses in groundnut yield and quality. Although large numbers of accessions were identified as rust resistant in wild, interspecific derivative and cultivated groundnut species, transfer of resistance to well-adapted cultivars is limited due to linkage drag, which worsens yield potential and market acceptance. A F2 mapping population comprising 117 individuals was developed from a cross between the rust resistant parent VG 9514 and rust susceptible parent TAG 24. Rust resistance was governed by single dominant gene in this cross. We identified 11 (out of 160) RAPD primers that exhibited polymorphism between these two parents. Using a modified bulk segregant analysis, primer J7 (5′CCTCTCGACA3′) produced a single coupling phase marker (J71350) and a repulsion phase marker (J71300) linked to rust resistance. Screening of the entire F2 population using primer J7 revealed that the coupling phase marker J71350 was linked with the rust resistance gene at a distance of 18.5 cM. On the other hand, the repulsion phase marker J71300 was completely linked with rust resistance. Additionally, both J71300 (P = 0.00075) and J71350 (P < 0.00001) were significantly associated with the rust resistance. Marker J71300 identified all homozygous rust resistant genotypes in the F2 population and was present in all the eight susceptible genotypes tested for validation. Thus, J71300 should be applicable for marker-assisted selection (MAS) in the groundnut rust resistance breeding programme in India. To the best of our knowledge this is the first report on the identification of RAPD markers linked to rust resistance in groundnut.

Keywords

Bulk segregant analysis Inheritance Peanut Puccinia arachidis resistance RAPD markers 

Notes

Acknowledgements

The authors thank Dr Niranjan Baisakh and Dr B.D. Singh for their critical comments and the International Atomic Energy Agency, Vienna for partial financial support under the Coordinated Research Project No. 12818.

References

  1. Bromfield KR, Bailey WK (1972) Inheritance of resistance to Puccinia arachidis in peanut. Phytopathology 62:748Google Scholar
  2. Burrow MD, Simpson CE, Paterson AH, Starr JL (1996) Identification of peanut (Arachis hypogaea L.) RAPD markers diagnostic of root-knot nematode (Meloidigyne arenaria (Neal) Chitwood) resistance. Mol Breed 2:368–379CrossRefGoogle Scholar
  3. FAOSTAT (2004) Available at http://apps.fao.org/cgi-bin/nph-do.pl?subset = agriculture
  4. Garcia GM, Stalker HT, Shroeder E, Kochert GA (1996) Identification of RAPD, SCAR and RFLP markers tightly linked to nematode resistance genes introgressed from Arachis cardenasii to A. hypogaea. Genome 39:836–845PubMedGoogle Scholar
  5. Gowda MVC, Motagi BN, Naidu GK, Diddimani SB, Sheshagiri R (2002) GPBD 4: a Spanish bunch groundnut genotype resistant to rust and late leaf spot. Int Arachis Newslett 22:29–32Google Scholar
  6. Haley SD, Miklas PN, Stavely JR, Byrum J, Kelly JD (1993) Identification of RAPD markers linked to a major rust resistance gene block in common bean. Theor Appl Genet 86:505–512CrossRefGoogle Scholar
  7. Halward TM, Stalker HT, Larue EA, Kochert G (1991) Genetic variation detectable with molecular markers among unadapted germplasm resources of cultivated peanut and related wild species. Genome 34:1013–1020Google Scholar
  8. Halward TM, Stalker HT, Larue EA, Kochert G (1992) Use of single primer DNA amplifications in genetic studies of peanut (Arachis hypogaea L.). Plant Mol Biol 18:315–323PubMedCrossRefGoogle Scholar
  9. He G, Prakash CS (1997) Identification of polymorphic DNA markers in cultivated peanut (Arachis hypogaea L.). Euphytica 97:143–149CrossRefGoogle Scholar
  10. Herselman L (2003) Genetic variation among Southern African cultivated peanut (Arachis hypogaea L.) genotypes as revealed by AFLP analysis. Euphytica 133:319–327CrossRefGoogle Scholar
  11. Herselman L, Thwaites R, Kimmins FM, Courtois B, Van der Merwe PJA, Seal SE (2004) Identification and mapping of AFLP markers linked to peanut (Arachis hypogaea L.) resistance to the aphid vector of groundnut rosette disease. Theor Appl Genet 109:1426–1433 Google Scholar
  12. Johnson E, Miklas PN, Stavely JR, Matinez-Cruzado H (1995) Coupling and repulsion phase RAPDs for marker assisted selection of PI 181996 rust resistance in common bean. Theor Appl Genet 90:659–664CrossRefGoogle Scholar
  13. Knauft DA (1987) Inheritance of rust resistance in groundnut. In: Groundnut rust disease: proceedings of a discussion group meeting 24–28th Sept. 1984. ICRISAT, Patancheru, pp183–187Google Scholar
  14. Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175Google Scholar
  15. Kotresh H, Fakrudin B, Punnuri SM, Rajkumar BK, Thudi M, Paramesh H, Lohithaswa H, Kuruvinashetti MS (2006) Identification of two RAPD markers genetically linked to a recessive allele of a Fusarium wilt resistance gene in Pigeonpea (Cajanus cajan L. Mill sp.). Euphytica 149:113–120CrossRefGoogle Scholar
  16. Lincoln SE, Daly MJ, Lander ES (1993) Constructing genetic linkage maps with MAPMAKER EXP V3.0: a tutorial and reference manual. A whitehead Institute of Biomedical research technical reporthttp://www.mapmaker@genome.wi.mit.edu
  17. Mace ES, Phong DT, Upadhyaya HD, Chandra S, Crouch JH (2006) SSR analysis of cultivated groundnut (Arachis hypogaea L.) germplasm resistant to rust and late leaf spot diseases. Euphytica 152:317–330CrossRefGoogle Scholar
  18. Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832PubMedCrossRefGoogle Scholar
  19. Mondal S, Ghosh S, Badigannavar AM (2005) RAPD polymorphism among groundnut genotypes differing in disease reaction to late leaf spot and rust. Int Arachis Newslett 25:27–30Google Scholar
  20. Naidu RA, Kimmins FM, Deom CM, Subrahmanyam P, Chiyembekeza AJ, van der Merwe PJA (1999) Groundnut rosette. A virus disease affecting groundnut production in sub-Saharan Africa. Plant Disease 83:700–709CrossRefGoogle Scholar
  21. Nigam SN, Dwivedi SL, Gibbons RW (1980) Groundnut Breeding at ICRISAT. In: Proceedings international workshop on groundnut, 13–17th Oct 1980. ICRISAT, India, pp62–68Google Scholar
  22. Pande S, Rao NJ (2001) Resistance of wild Arachis species to late leaf spot and rust in greenhouse trials. Plant Disease 85:851–855CrossRefGoogle Scholar
  23. Patil SH, Kale DM, Deshmukh SN, Fulzele GR, Weginwar BG (1995) Semi-dwarf, early maturing and high yielding new groundnut variety, TAG-24. J Oilseed Res 12:254–257Google Scholar
  24. Raina SN, Rani V, Kojima T, Ogihara Y, Singh KP, Devarumath RM (2001) RAPD and ISSR fingerprint as useful genetic markers for analysis of genetic diversity, varietal identification and phylogenetic relationship in peanut (Arachis hypogaea L.) cultivars and wild species. Genome 44:763–772PubMedCrossRefGoogle Scholar
  25. Saghai-Maroof MA, Saliman KM, Jorgensen RA, Allard RW (1984) Ribisomal DNA spacer length polymorphism in barley: mendelian inheritance, chromosomal location and population dynamics. Proc Natl Acad Sci USA 81:8014–8018PubMedCrossRefGoogle Scholar
  26. SAS Institute Inc (1998) SAS/STAT user’s guide, Release 6.03 ed. SAS Institute Inc, Cary, NCGoogle Scholar
  27. Singh AK, Subrahmanyam P, Moss JP (1984) The dominant nature of resistance to Puccinia arachidis in certain wild Arachis species. Oleagineux 39:535–537Google Scholar
  28. Subrahmanyam P, McDonald D (1982) Groundnut rust- its survival and carry-over in India. Proc Indian Acad Sci (Plant Sci) 91:93–100Google Scholar
  29. Subrahmanyam P, McDonald D, Waliyar F, Reddy LJ, Nigam SN, Gibbons RW, Rao VR, Singh AK, Pande S, Reddy PM, Subba Rao PV (1995) Screening methods and sources of resistance to rust and late leaf spot of groundnut. In: Information bulletin no. 47. ICRISAT, Patancheru IndiaGoogle Scholar
  30. Subrahmanyam P, Rao VR, Mcdonald D, Moss JP, Gibbons RW (1989) Origins of resistance to rust and late leaf spot in peanut (Arachis hypogaea Fabaceae). Econ Bot 43:444–455Google Scholar
  31. Subramanian V, Gurtu S, Nageswara Rao RC, Nigam SN (2000) Identifiaction of DNA polymorphism in cultivated groundnut using random amplified polymorphic DNA (RAPD) assay. Genome 43:656–660PubMedCrossRefGoogle Scholar
  32. Tanksley SD (1983) Molecular markers in plant breeding. Plant Mol Biol Rep 1:3–8CrossRefGoogle Scholar
  33. Varman PV (1999) A foliar disease resistant line developed through interspecific hybridization in groundnut (Arachis hypogaea). Indian J Agri Sci 69:67–68Google Scholar
  34. Vijayalakshmi S, Yadav K, Kushwaha C, Sarode SB, Srivastava CP, Chand R, Singh BD (2005) Identification of RAPD markers linked to the rust (Uromyces fabae) resistance gene in pea (Pisum sativum). Euphytica 144:265–274CrossRefGoogle Scholar
  35. Vindhiyavarman P, Raveendran TS, Ganapathi T (1993) Inheritance of rust resistance in groundnut. Madras Agri J 80:175–176Google Scholar
  36. Wu JY, Wu HK, Mei-Chu Chung (2002) Co-dominant RAPD markers closely linked with two morphological genes in rice (Oryza sativa L.). Bot Bull Acad Sin 43:171–180Google Scholar
  37. Ynturi P, Jenkins JN, McCarty JC, Gutierrez OA, Saha S (2006) Association of root knot nematode resistance genes with simple sequence repeat markers on two chromosomes in cotton. Crop Sci 46:2670–2674CrossRefGoogle Scholar
  38. Yong L, Boshou L, Wang SY, Dong L, Jiang H (2005) Identification of AFLP markers for resistance to seed infection by Aspergillus flavus in peanut (Arachis hypogaea L.). Acta Agronomica Sinica 31:1349–1353Google Scholar
  39. Young RA, Kelly JD (1997) RAPD markers linked to three major anthracnose resistance genes in common bean. Crop Sci 37:940–946CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Suvendu Mondal
    • 1
  • Anand M. Badigannavar
    • 1
  • G. S. S. Murty
    • 1
  1. 1.Nuclear Agriculture and Biotechnology DivisionBhabha Atomic Research CentreMumbaiIndia

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