Diallel analysis of floral morphology in radish (Raphanus sativus L.)
- 136 Downloads
We made a 5 × 5 diallel set of crosses between five inbred lines, four Japanese and one Chinese, and diallel analyses of radish floral morphology were conducted to estimate the genetic parameters underlying 13 floral traits. The modes of inheritance of 12 traits (but not anther length on the long stamen) followed the additive-dominance model. Broad-sense heritabilities of the 12 traits were 0.93–0.99, suggesting that floral morphology is highly heritable. But narrow-sense heritabilities differed among traits, ranging from 0.34 to 0.89. Pistil and stamen heights, tube length, and stigma position were explained mainly by additive effects, but anther, stigma, petal, and whole flower sizes were affected greatly by dominance effects as well as by additive effects. The correlations between the 13 traits suggested that some floral traits (e.g. stigma size and position) are more or less independent, indicating the possibility of creating lines with new floral morphologies by selecting for these genetically independent traits. A flower with a larger and higher stigma might improve the production of cross-pollinated seeds through enhancement of pollination efficiency. In that case, by selecting for stigma position in an early segregating generation and for stigma size in a later generation, it should be possible to produce new parental lines with effective floral morphology for F 1 seed production.
KeywordsDiallel analysis Floral morphology Mode of inheritance Pollination efficiency Radish Raphanus sativus L.
We thank Dr Y. Ukai, a former professor at the University of Tokyo, for valuable advice on the analyses and for fruitful comments on this article, and Dr S. Matsuura, of the Tohoku Seed Company, for useful comments on this study. This work was supported by a grant for a Research Project for Utilizing Advanced Technology in Agriculture, Forestry and Fisheries from the Ministry of Agriculture, Forestry and Fisheries of Japan and by a Grant-in-Aid for Exploratory Research from the Japan Society for the Promotion of Science.
- Falconer DS (1989) Introduction to quantitative genetics, 3rd edn. Longman, LondonGoogle Scholar
- Iwata H, Niikura S, Matsuura S et al (2000) Diallel analysis of root shape of Japanese radish (Raphanus sativus L.) based on elliptic Fourier descriptors. Breed Sci 50:73–80Google Scholar
- Jones RM (1965) Analysis of variance of the half diallel table. Heredity 20:117–121Google Scholar
- Kitamura S (1958) Daikon no hinsyu to sono hensen (varieties of radish and transitions between them). In: Nishiyama I (ed) Nihon no daikon (Japanese radish). Nihongakujyutsu Sinkoukai, Tokyo, pp 1–19Google Scholar
- Mather K, Jinks JL (1982) Chapter 9, Diallels. In Biometrical Genetics, 3rd edn. Chapman and Hall, London, pp 255–291Google Scholar
- Nishiyama I, Nei M, Miyaji Y et al. (1958) Idengaku-teki kenkyu (Genetical studies). In: Nishiyama I (ed) Nihon no daikon (Japanese radish). Nihongakujyutsu Shinkokai, Tokyo, pp 98–131Google Scholar
- Richards AJ (1997) Plant Breeding Systems, 2nd edn. Chapman & Hall, LondonGoogle Scholar
- Ukai Y (1989) A microcomputer program DIALL for diallel analysis of quantitative characters. Jpn J Breed 39:107–109Google Scholar
- Ukai Y (2002) Ryouteki keishitsu no idenkaiseki [Genetic analysis of quantitative traits]. Igaku-shuppan, TokyoGoogle Scholar