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Molecular identification of Pm12-carrying introgression lines in wheat using genomic and EST-SSR markers

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Abstract

Pm12, transferred from Aegilops speltoides (2n = 2x = 14, genome SS) to wheat, confers effective resistance to powdery mildew worldwide. By applying bulked segregant analysis in a BC3F2 segregating population consisting of 305 plants, 18 wheat genomic and EST-SSR markers linked to the resistance gene were identified. Pm12 was located in the 6SS portion of the T6BS-6SS.6SL translocation chromosome based on the physical bin positions of the genomic and EST-SSR markers in the Chinese Spring group six deletion stocks and their linkage relationship to the resistance gene. Twenty eight recombinants among 305 F2 plants indicated a low frequency of recombination between the alien chromosome segment and wheat chromosome 6B. Since recombination events occurred on both sides of Pm12, the materials generated provide opportunities for further reduction of alien chromatin by intercrossing selected individuals and using markers to select the required plants.

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Acknowledgements

The authors are grateful to Dr. R. McIntosh for improving the manuscript. This work was financially supported by the National Fund for Distinguished Young Scholars (30425039), National Natural Science Foundation of China (30200174, 30571151), Fok Ying Tung Education Foundation (94021) and the State High Tech Programs (2006AA100102, 2006AA10Z1E9, 2006AA10Z1C4).

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  1. Hao Xie

    Present address: Beijing University of Agriculture, Beijing, 102206, P. R. China

Authors and Affiliations

  1. Department of Plant Genetics & Breeding and State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis Research & Untilization, Ministry of Education, China Agricultural University, Beijing, 100094, P. R. China

    Wei Song, Hao Xie, Qing Liu, Chaojie Xie, Zhongfu Ni, Tsomin Yang, Qixin Sun & Zhiyong Liu

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  1. Wei Song
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Correspondence to Qixin Sun or Zhiyong Liu.

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Wei Song and Hao Xie are contributed equally to this work.

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Song, W., Xie, H., Liu, Q. et al. Molecular identification of Pm12-carrying introgression lines in wheat using genomic and EST-SSR markers. Euphytica 158, 95–102 (2007). https://doi.org/10.1007/s10681-007-9432-4

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