Molecular identification of Pm12-carrying introgression lines in wheat using genomic and EST-SSR markers
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Pm12, transferred from Aegilops speltoides (2n = 2x = 14, genome SS) to wheat, confers effective resistance to powdery mildew worldwide. By applying bulked segregant analysis in a BC3F2 segregating population consisting of 305 plants, 18 wheat genomic and EST-SSR markers linked to the resistance gene were identified. Pm12 was located in the 6SS portion of the T6BS-6SS.6SL translocation chromosome based on the physical bin positions of the genomic and EST-SSR markers in the Chinese Spring group six deletion stocks and their linkage relationship to the resistance gene. Twenty eight recombinants among 305 F2 plants indicated a low frequency of recombination between the alien chromosome segment and wheat chromosome 6B. Since recombination events occurred on both sides of Pm12, the materials generated provide opportunities for further reduction of alien chromatin by intercrossing selected individuals and using markers to select the required plants.
KeywordsPowdery mildew resistance Pm12 Aegilops speltoides SSR Introgression lines
The authors are grateful to Dr. R. McIntosh for improving the manuscript. This work was financially supported by the National Fund for Distinguished Young Scholars (30425039), National Natural Science Foundation of China (30200174, 30571151), Fok Ying Tung Education Foundation (94021) and the State High Tech Programs (2006AA100102, 2006AA10Z1E9, 2006AA10Z1C4).
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