Abstract
Two wide hybrids, Diplotaxis erucoides (2n = 14) × Brassica rapa (2n = 20) and B. maurorum (2n = 20) × B. rapa, were developed using the sequential ovary–ovule culture. Reciprocal crosses failed, possibly as a consequence of strong unilateral incompatibility. The F 1 hybrids in each combination were completely male sterile and morphologically intermediate to the respective parents. DNA marker polymorphism and chromosome counts confirmed their hybrid nature. High frequency of bivalents in the F 1 and the presence of trivalents/quadrivalents in the derived amphiploids suggested genomic duplications and homoeology of the parental genomes. Up to three homoeologous pairs between the D. erucoides (DeDe) and B. rapa (AA) genomes, and one between B. maurorum (BmBm) and B. rapa genomes were observed. Successful synthesis of the F 1 hybrids and amphiploids of B. rapa with D. erucoides and B. maurorum, and allosyndetic chromosome pairing are expected to permit introgressions of desirable loci into the cultivated Brassica germplasm, especially for resistance to Alternaria brassicae and Albugo candida.
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Acknowledgements
This study was partly supported by the funds provided under the Indian Council of Agricultural Research aided research project “National network for management of Alternaria blight in Brassica juncea and vegetable crops”. Sincere thanks to Prof. Adam Lukaszewski for helpful suggestions. Gratitude is also expressed to Prof. Shyam Prakash for the supply of seed samples of the wild crucifers.
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Garg, H., Banga, S., Bansal, P. et al. Hybridizing Brassica rapa with wild crucifers Diplotaxis erucoides and Brassica maurorum . Euphytica 156, 417–424 (2007). https://doi.org/10.1007/s10681-007-9391-9
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DOI: https://doi.org/10.1007/s10681-007-9391-9