Abstract
Jatropha curcas (Euphorbiaceae) is an oil-bearing species with multiple uses and considerable potential as a bioenergy crop. The present investigation has been undertaken to assess the extent of genetic diversity in a representative set of 42 accessions of J. curcas encompassing different crop growing regions in India along with a non-toxic genotype from Mexico as a prelude for utilization of promising and genetically divergent materials in the breeding programmes. Molecular polymorphism was 42.0% with 400 RAPD primers and 33.5% with 100 ISSR primers between accessions indicating modest levels of genetic variation in the Indian germplasm. The within-population variation based on RAPD polymorphism was 64.0% and was on par with the inter-population variation. Polymorphic ISSR markers have been identified that could differentiate the Indian accessions from the Mexican genotype and two of them were converted to SCAR markers. The SCAR primer pair ISPJ1 amplified a 543 bp fragment in all the Indian populations, while ISPJ2 with a specific amplicon of 1,096 bp was specific to the Mexican genotype. Population-specific bands have been identified for the accession from Kerala (2 RAPD markers), Neemuch-1 from Rajasthan (1 each of RAPD and ISSR markers) and the non-toxic genotype from Mexico (17 RAPD and 4 ISSR markers), which serve as diagnostic markers in genotyping. The study indicates an immediate need for widening the genetic base of J. curcas germplasm through introduction of accessions with broader geographical background.
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Acknowledgements
This research is supported by the RSAD project on J. curcas sponsored by the Government of Andhra Pradesh, India. The authors thank the Principal Investigators of the cooperating centers of the RSAD project for sparing the Indian accessions and Prof. Klaus Becker, University of Hohenheim, Stuttgart, Germany for the non-toxic Mexican genotype. The authors thank the Project Director, Directorate of Oilseeds Research for extending all the facilities for carrying out the investigation and Dr. V. Dinesh Kumar (DOR) and Dr. J. Hu (USDA, USA) for their helpful suggestions during this investigation.
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Basha, S.D., Sujatha, M. Inter and intra-population variability of Jatropha curcas (L.) characterized by RAPD and ISSR markers and development of population-specific SCAR markers. Euphytica 156, 375–386 (2007). https://doi.org/10.1007/s10681-007-9387-5
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DOI: https://doi.org/10.1007/s10681-007-9387-5