, Volume 158, Issue 3, pp 287–294 | Cite as

Genetic markers for doubled haploid response in barley

  • Xi-Wen Chen
  • Luís Cistué
  • María Muñoz-Amatriaín
  • Miguel Sanz
  • Ignacio Romagosa
  • Ana-María Castillo
  • María-Pilar Vallés


In order to analyse the genetic control of anther culture response in barley, a doubled-haploid (DH) population from the cross between a medium responsive cultivar ‘Dobla’ and the model cultivar ‘Igri’ was produced. A linkage map was constructed with 91 markers. A sub-population of 41 lines was characterised for different components of the anther culture response, and was used for quantitative trait loci (QTL) analysis. The vrs1 locus region on chromosome 2H, which determines inflorescence row type, was coincident with the largest putative QTL for number of embryos (nEMB) and albino plants. A region of chromosome 6H was associated with QTLs for nEMB and green plants. QTLs for number and percentage of green plants were located on the long arm of chromosome 5H. Therefore, new QTLs for main components of barley anther culture response were identified on chromosomes 2H, 5H and 6H, indicating that anther culture response in barley could be controlled by relative few genes of large effect. This work is a useful step towards the identification of new regions on the barley genome that could be associated with fundamental biological process implicated in the anther culture response.


Anther culture Barley Doubled haploid (DH) Microspore embryogenesis QTLs 



X-W. Chen was recipient of a fellowship form the Ministerio de Educación y Cultura from the Spanish government. The research was supported by Projects PB97-1159, and AGL2001-1631 from Plan Comisión Interministerial de Ciencia y Tecnología of Spain.


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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Xi-Wen Chen
    • 1
    • 3
  • Luís Cistué
    • 1
  • María Muñoz-Amatriaín
    • 1
  • Miguel Sanz
    • 1
  • Ignacio Romagosa
    • 2
  • Ana-María Castillo
    • 1
  • María-Pilar Vallés
    • 1
  1. 1.Departamento de Genética y Producción Vegetal, Estación Experimental de Aula DeiCSICZaragozaSpain
  2. 2.Area de Conreus ExtensiusCentre UdL-IRTALleidaSpain
  3. 3.Department of Biochemistry and Molecular BiologyNankai UniversityTianjinPeople’s Republic of China

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