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Euphytica

, Volume 158, Issue 3, pp 313–321 | Cite as

Molecular markers to assess genetic diversity

  • Alan H. Schulman
Article

Abstract

Molecular markers play an essential role today in all aspects of plant breeding, ranging from the identification of genes responsible for desired traits to the management of backcrossing programs. The emergence of marker systems has, for the last 30 years, closely tracked developments in biochemistry and molecular biology. Following the demonstration that retrotransposons are ubiquitous, active, and abundant in plant genomes, markers were developed that are based on the insertional polymorphism they create upon replication. They virtually all exploit the joint that is formed, during retrotransposon integration, between genomic DNA and the long terminal repeats at either end of retrotransposon. The various retrotransposon marker systems differ in the nature of the second primer used in the amplification reactions. All except one of these marker methods is dominant (RBIP is co-dominant). Hence, the availability of effective means to generate doubled haploid populations through gametophytic embryogenesis is valuable for the efficient use of these markers. Over the last eight years, retrotransposon-based markers have been developed for crop species and trees across the plant kingdom, as well as for fungi and insects. Many retrotransposons features make them appealing as the basis of molecular marker systems. They are usually dispersed throughout the genome and produce large genetic changes at the point of insertion that can be detected with family-specific amplification primers. The ancestral state of a retrotransposon insertion is known, and subsequent changes at the locus are not subject to homoplasy. Retrotransposon markers are useful in accelerating backcrossing programs, tagging genes of interest, tracking germplasm, verifying and producing pedigrees, and examining crop evolution.

Keywords

Molecular marker Retrotransposon IRAP REMAP SSAP RBIP 

Abbreviations

AFLP®

amplified fragment length polymorphism

DH

double haploid

IRAP

inter-retrotransposon amplified polymorphism

LTR

long terminal repeat

PIC

polymorphism information content

RAPD

randomly amplified polymorphic DNA

RBIP

retrotransposon-based amplified polymorphism

REMAP

retrotransposon-microsatellite amplified polymorphism

RFLP

restriction-fragment length polymorphism

SNP

single nucleotide polymorphism

SSAP

sequence-specific amplified polymorphism

SSR

simple sequence repeat

Notes

Acknowledgements

This work is a contribution under COST Action 851: Gametic Cells and Molecular Breeding for Crop Improvement. Much of the work cited was carried out under EU contracts QLK5-2000-01502 (TEGERM) and QLRT-1999-01499 (MMEDV) as well as through the ESGEMO project supported by the Academy of Finland and the Ministry of Agriculture and Forestry of Finland. Anne-Mari Narvanto and Ursula Lönnqvist are thanked for their always excellent technical assistance.

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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  1. 1.Biotechnology and Food Research, MTT Agrifood Research Finland and MTT/BI Plant Genomics Laboratory, Institute of BiotechnologyUniversity of Helsinki and MTT Agrifood Research FinlandHelsinkiFinland

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