, Volume 151, Issue 1, pp 49–61 | Cite as

Genetics of resistance to 3 isolates of Ascochyta fabae on Faba bean (Vicia faba L.) in controlled conditions

  • Mohamed Kharrat
  • Joël Le Guen
  • Bernard Tivoli


Genetics of resistance to Ascochyta fabae Speg. in Vicia faba L. was studied with a final objective to develop resistant faba bean varieties to Ascochyta blight. The study was conducted separately on 3 single spore isolates (AF10-2 and AF13-2 from Tunisia and AF4-3 from France) and belonging to different groups of virulence (GV1 and GV2). Important general combining ability (GCA) effects were found especially with isolates AF10-2 and AF4-3. Specific combining ability (SCA), although significant for the 3 isolates, was important only with AF13 -2, but less important than GCA. Additive gene effects were predominant to non-additive effects. Lines 29H and A8817 transmitted to their progenies resistance to the 3 isolates, whereas 14–12 and 19TB conferred resistance to their progenies only with isolates AF13-2 and AF4-3, respectively. In the material studied, resistance was generally controlled by dominant genes but also could be attributed to recessive genes although less frequent. Analysis of segregation in the F2 of 2 crosses between the resistant lines (A8817 and 29H) and the susceptible line (14–12) with isolate AF4-3 revealed dominant monogenic control at the level of leaves in the 2 resistant lines and, in addition, a recessive gene controlling resistance of stems. Non-allelic interactions were occasionally manifested and their origin appeared to be due to line 19TB. A recurrent selection scheme was proposed with the objective to develop improved open-pollination populations and synthetic varieties responding to the objective of the national Tunisian research programme on faba bean.

Key words

Ascochyta fabae diallel analysis faba bean genetic resistance Vicia faba 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Belqadi, L., 2003. Diversité, conservation et valorisation des ressources génétiques marocaines de fève (Vicia faba L.). PhD thesis, Institut Agronomique et Vétérinaire Hassan II, Rabat, Morocco, 138p.Google Scholar
  2. Bond, D.A. & M. Pope, 1980. Ascochyta fabae on winter beans (Vicia faba): Pathogen spread and variation in host resistance. Plant Pathology 29: 59–65.Google Scholar
  3. Clulow, S.A., B.G. Lewis & P. Mathews, 1992. Expression of resistance to Mycosphaerella pinodes in Pisum sativum. Plant Pathology 41: 362–369.Google Scholar
  4. Diekmann, M.B., 1982. Survey on pests and diseases of faba beans (Vicia faba L.) in Egypt, Morocco and Tunisia. Fabis Newsletter 4: 44–45.Google Scholar
  5. Gaunt, R.E., 1983. Shoot diseases caused by fungal pathogens. In: P.D. Hebblethwaite (ed.), The Faba Bean (Vicia faba L.), pp. 463–492. Butterworths.Google Scholar
  6. Griffing, B., 1956. Concept of general and specific combining ability in relation to diallel crossing systems. Australian Journal of Biological Science 9: 463–493.Google Scholar
  7. Hampton, J.G., 1980. The significance of Ascochyta fabae in broad beans in the Manawatu, and methods for its control. New Zealand Journal of Experimental Agriculture 8: 305–308.Google Scholar
  8. Hanounik, S.B. & N.F. Maliha, 1984. Resistance in Vicia faba to Ascochyta fabae. Fabis Newsletter 9: 33–36.Google Scholar
  9. Hanounik, S.B. & L.D. Robertson, 1989. Resistance in Vicia faba germ plasm to blight caused by Ascochyta fabae. Plant Disease 73(3): 202–205.Google Scholar
  10. Hayman, B.I., 1954a. The analysis of diallel tables. Biometrics 10: 235–244.CrossRefGoogle Scholar
  11. Hayman, B.I., 1954b. The theory and analysis of diallel crosses. Genetics 39: 789–809.Google Scholar
  12. Hayman, B.I., 1958. The theory and analysis of diallel crosses II. Genetics 43: 63–85.PubMedGoogle Scholar
  13. Iqbal, S.M., A. Ghafoor, M. Bashir & A. Baksh, 1988. Reaction of faba bean genotypes to various diseases in Pakistan. Fabis Newsletter 21: 40–42.Google Scholar
  14. Jellis, G.J. & E. Punithalingam, 1991. Discovery of Didymella fabae sp. nov., the teleomorph of Ascochyta fabae, on faba bean straw. Plant Pathology 40: 150–157.Google Scholar
  15. Jellis, G.J., G. Lockwood & R.G. Aubury, 1984a. Further evaluation of chlorothalonil for control of Ascochyta fabae in Faba beans. Annals of Applied Biology 104: 58–59 (supplement).Google Scholar
  16. Jellis G.J., G. Lockwood & R.G. Aubury, 1984b. Resistance to Ascochyta blight (Ascochyta fabae) in a winter-hardy line of faba bean (Vicia faba equina). Fabis Newsletter 10: 27–29.Google Scholar
  17. Jinks, J.L., 1954. The analysis of continuous variation in a diallel cross of Nicotiana rustica varieties. Genetics 39: 767–788.PubMedGoogle Scholar
  18. Jinks, J.L., 1956. The F2 and backcross generations from a set of diallel crosses. Heredity, 10: 1–30.Google Scholar
  19. Kamel, A., H. Halila, H. Ben Salah, M. Harrabi & M. Deghaies, 1989. Faba bean diseases in Tunisia. Fabis Newsletter 24: 29–32.Google Scholar
  20. Kharbanda, P.D. & C.C. Bernier, 1979. Effectiveness of seed and foliar application of fungicides to control Ascochyta blight of faba beans, Candian Journal of Plant Science 59: 661–666.Google Scholar
  21. Kharrat, M., I. Amri, M. Chérif & M. Harrabi, 1996. Surveillance des maladies de la fève en Tunisie. In: B. Ezzahiri, A. Lyamani, A. Farh & M. El Yamani (Eds.), pp. 21–26. Proc. of regional symposium on cereal and food legume diseases, 11–14 November 1996, Rabat, Morocco.Google Scholar
  22. Kharrat, M., J. Le Guen & B. Tivoli, 2000a. Genetic of resistance to Ascochyta fabae on faba bean (Vicia faba L.). Petria 10: 241–245.Google Scholar
  23. Kharrat, M., B. Tivoli & J. Le Guen, 2000b. Characterisation of Tunisian Ascochyta fabae isolates, causal agent of blight of faba bean. Annales de l'INRAT 73: 91–104.Google Scholar
  24. Kohpina, S., R. Knight & F.L. Stoddard, 2000. Genetics of resistance to ascochyta blight in two populations of faba bean. Euphytica 112(2): 101–107.CrossRefGoogle Scholar
  25. Lockwood, G., G.J. Jellis & R.G. Aubury, 1985. Genotypic influences on the incidence of infection by Ascochyta fabae in winter-hardy faba beans (Vicia faba). Plant Pathology 34: 341–346.Google Scholar
  26. Madeira, A.C., J.A. Clark & S. Rossall, 1988. Growth, light interception and disease in field bean (Vicia faba): The effect of late infection by Ascochyta fabae. Annals of Applied Biology 112: 585–595.CrossRefGoogle Scholar
  27. Mather, K. & J.L. Jinks, 1982. Biometrical genetics: The study of continuous variation. 3rd (ed.) Chapman and Hall Publishers, New York.Google Scholar
  28. Maurin, N., 1989. Biologie d'Ascochyta fabae Speg. et étude des relations hôte-parasite en vue de l'appréciation de la résistance de la féverole à l'anthracnose. PhD Thesis, Univ. of Rennes I, France, p. 133.Google Scholar
  29. Maurin, N. & B. Tivoli, 1992. Variation in the resistance of Vicia faba to Ascochyta fabae in relation to disease development in field trials. Plant Pathology 41: 737–744.Google Scholar
  30. Maurin, N., B. Tivoli & C. Onfroy, 1990. Mieux connaître les maladies et leur agent pathogène pour mieux les combattre: Exemple de l'anthracnose de la féverole. Perspectives Agricoles 146: 36–45.Google Scholar
  31. Metoui, O., A. Porta-Puglia, M. Marrakchi, M. Kharrat & R. Angelini, 2002. The Vicia faba diamine oxydase system and its role in response to Ascochyta fabae and to wounding. Journal of Plant Pathology 84(1): 19–25.Google Scholar
  32. Pritchard, P.R., P.S. Rowe & S. Rossall, 1989. A comparison of infection of resistant and susceptible lines of field bean (Vicia faba) by Ascochyta fabae. Plant Pathology 38: 266–270.Google Scholar
  33. Ramsey, M., R. Knight & J. Paull, 1995. Ascochyta and chocolate spot resistant faba beans (Vicia faba L.) for Australia. In: Improving production and utilisation of grain legumes. Proc. of the 2nd European conference on grain legumes, p 164–165, 9–13 July 1995, Copenhagen, Denmark.Google Scholar
  34. Rashid, K.Y., C.C. Bernier & R.L. Conner, 1991a. Genetics of resistance in faba bean inbred lines to five isolates of Ascochyta fabae. Canadian Journal of Plant Pathology 13: 218–225.CrossRefGoogle Scholar
  35. Rashid, K.Y., C.C. Bernier & R.L. Conner, 1991b. Evaluation of fava bean for resistance to Ascochyta fabae and development of host differentials for race. Plant Disease 75(8): 852–855.]Google Scholar
  36. Roman, B., Z. Satovic, C.M. Avila, D. Rubiales, M.T. Moreno, & A.M. Torres, 2003. Locating genes associated with Ascochyta fabae resistance in Vicia faba. Australian Journal of Agricultural Research 54: 85–90.CrossRefGoogle Scholar
  37. Sillero, J.C., C.M. Avila, M.T. Moreno & D. Rubiales, 2001. Identification of resistance to Ascochyta fabae in Vicia faba germplasm. Plant Breeding 120(6): 529–531.CrossRefGoogle Scholar
  38. Slim Khaldi, R. & S. Zekri, 2002. Etude des légumineuses alimentaires dans les systèmes de production du nord de la Tunisie:Situation actuelle et possibilités de développement. R. Slim Khaldi & S. Zekri (eds.), 92 p., La page Infographique, Tunisie.Google Scholar
  39. Tivoli, B., B. Reynaud, N. Maurin, P. Berthélem & J. Le Guen, 1987. Comparison of some methods for evaluation of reaction of different faba bean genotypes to Ascochyta fabae. Fabis Newsletter 17: 35–38.Google Scholar
  40. Tivoli, B., P. Berthélem, J. Le Guen & C. Onfroy, 1988. A study of the performance of certain faba bean genotypes in relation to Botrytis fabae and Ascochyta fabae in France. Fabis Newsletter 21: 36–40.Google Scholar
  41. Van Breukelen, E.W.M., 1985. Screening faba beans for resistance to Ascochyta fabae by artificial inoculation of seedlings. Euphytica 34: 425–430.CrossRefGoogle Scholar
  42. Yakop, U.M, J.G. Paul & M. Ramsey, 1997. Variation in the resistance of faba bean (Vicia faba L.) to Ascochyta blight in Australia. Abstract of a poster presented in the 3rd International Food legume Conference, Adelaide, Australia, (22–26 September 1997), p.159, book of abstracts.Google Scholar

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Mohamed Kharrat
    • 1
  • Joël Le Guen
    • 2
  • Bernard Tivoli
    • 2
  1. 1.INRATField Crop Lab.ArianaTunisia
  2. 2.INRADomaine de la MotteLe Rheu Cedex RennesFrance

Personalised recommendations