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The Introduction of Transgenes to Control Blackheart in Pineapple (Ananas Comosus L.) cv. Smooth Cayenne by Microprojectile Bombardment

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Summary

A transformation technique for the introduction of transgenes to control blackheart by particle bombardment has been developed for pineapple cv. Smooth Cayenne. Leaf callus cultures capable of high frequency organogenesis with a short regeneration time were used as explant material. Gus and gfp reporter genes were used to observe and determine transient and stable expression. The ppo gene, isolated from pineapple, was introduced to control blackheart. Co-transformation occurred with constructs containing the nptII gene conferring geneticin resistance. We have recovered 15 independent transgenic gus and gfp lines each from 8 separate experiments and 22 ppo lines from 11 experiments. Gus, gfp, ppo and nptII positive plants have been regenerated, which have been shown by Southern blot analysis to be stable transgenics containing multiple copies of the introduced genes. These results show that biolistic gene delivery in pineapple can be successfully achieved at an acceptable efficiency of 0.21–1.5% for genetic improvement of ’Smooth Cayenne’, the industry standard throughout the world.

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Abbreviations

BAP:

6-Benzyl amino purine

gfp :

Green fluorescent protein

gus :

β-glucuronidase

NAA:

α-Naphthalene acetic acid

nptII :

Neomycin phosphotransferase

ppo :

Polyphenol oxidase

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Authors and Affiliations

  1. Department of Primary Industries & Fisheries, Maroochy Research Station, PO Box 5083, S.C.M.C., Nambour, Queensland, 4560, Australia

    H. L. Ko, P. R. Campbell, M. P. Jobin-Décor, K. L. Eccleston & M. K. Smith

  2. Benitec Australia Ltd., PO Box 4193, St. Lucia, QLD 4067, Australia

    M. W. Graham

Authors
  1. H. L. Ko
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  2. P. R. Campbell
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  3. M. P. Jobin-Décor
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  4. K. L. Eccleston
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  5. M. W. Graham
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  6. M. K. Smith
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Correspondence to H. L. Ko.

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Ko, H.L., Campbell, P.R., Jobin-Décor, M.P. et al. The Introduction of Transgenes to Control Blackheart in Pineapple (Ananas Comosus L.) cv. Smooth Cayenne by Microprojectile Bombardment. Euphytica 150, 387–395 (2006). https://doi.org/10.1007/s10681-006-9124-5

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  • DOI: https://doi.org/10.1007/s10681-006-9124-5

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