Summary
Four different hybridization experiments were carried out to obtain interspecific hybrids with Spanish cultivars of lentil (Lens culinaris M.). In hybridization experiments I and II, undertaken with only pollination and pollination with the addition of gibberellic acid after fertilization, respectively, no lentil hybrids were recovered. A single interspecific hybrid with L. odemensis was obtained in experiment III using the embryo rescue protocol of Cohen et al. (1984), in this case, a crossing efficiency of 0.11% and a rescue efficiency of 2.5% were obtained. Hybridization experiment IV used a specific embryo rescue protocol developed in this study. In this experiment, ovule-embryos of 18 DAP were cultured on MS salts with 1% sucrose and 1 μ M IAA + 0.8 μ M KN; after two weeks, embryos were released from the ovular integuments and cultured on the same medium for another two weeks in upright position. Afterwards, the embryos were transferred to test tubes containing the same medium and one month later plantlets were obtained. Using the above protocol, out of a total of 1707 pollinations, 6 interspecific hybrids with L. odemensis, 2 with L. nigricans and one with L. ervoides were recovered, yielding on average a crossing efficiency of 0.53% and an average rescue efficiency of 8.26%. Taking into consideration only the interspecific crossing blocks in which hybrids were recovered, the crossing efficiency with L. odemensis was 9%, while with both L. nigircans and L. ervoides the crossing efficiencies were 3%. Rescue efficiencies based on hybrids recovered per number of ovules cultured ranged between 50–100%.
Similar content being viewed by others
Abbreviations
- DAP::
-
days after pollination
- GA3 :
-
gibberellic acid
- IAA:
-
indole-3-acetic acid
- KN::
-
kinetin
- PCR::
-
polymerase chain reaction
- RAPD:
-
random amplified polymorphic DNA
- ZEA::
-
zeatin
References
Abbo, S. & G. Ladizinsky, 1991. Anatomical aspects of hybrid embryo abortion in the genus Lens L. Botanical gazette 152: 316–320.
Abbo, S. & G. Ladizinsky, 1994. Genetical aspects of hybrid embryo abortion in the genus Lens L. Heredity 72: 193–200.
Ahmad, M., A.G. Fautrier, D.L. McNeil, D.J. Burritt & G.D. Hill, 1995. Attempts to overcome postfertilization barrier in interspecific crosses of the genus Lens. Plant Breeding 114: 558–560.
Alvarez, M.T., P. García & M. Pérez de la Vega, 1997. RAPD polymorphism in Spanish lentil landraces and cultivars. J Genet & Breed 51: 91–96.
Cohen, D., G. Ladizinsky, M. Ziv & F.J. Muehlbauer, 1984. Rescue of interspecific Lens hybrids by means of embryo culture. Plant Cell Tiss Org Cult 3: 343–347.
Dellaporta, S.L., J. Wood & J.B. Hicks, 1983. A plant DNA minipreparation: Version II. Plant Mol Biol Rep 1: 19–21.
Duran, Y., R. Fratini, P. García & M. Pérez de la Vega, 2004. An intersubspecific genetic map of Lens. Theor Appl Genet 108: 1265–1273.
Ferguson, M., 2000. Lens spp: Conserved resources, priorities for collection and future prospects. In: R. Knight (Ed.), Linking Research and Marketing Opportunities for Pulses in the 21st Century, Current Plant Science and Biotecnology in Agriculture, Vol. 34, pp. 613–620. Kluwer Academic Publishers, Dordrecht.
Ford, R., E.C.K. Pang & P.W.J. Taylor, 1997. Diversity analysis and species identification in Lens using PCR generated markers. Euphytica 96: 247–255.
Fratini, R. & M.L. Ruiz, 2002. Comparative study of different cytokinins in the induction of morphogenesis in lentil (Lens culinaris Medik.). In Vitro Cell Dev-Pl 38: 46–51.
Fratini, R. & M.L. Ruiz, 2003. A rooting procedure for lentil (Lens culinaris Medik.) and other hypogeous legumes (pea, chickpea and Lathyrus) based on explant polarity. Plant Cell Rep 21: 726–732.
Fratini, R., M.L. Ruiz & M. Pérez de la Vega, 2004. Intra-specific and inter-sub-specific crossing in lentil (Lens culinaris Medik.). Can J Plant Sci 84: 981–986.
Harlan, J.R. & J.M.J. de Wet, 1977. Pathways of genetic transfer from Tripsacum to Zea Mays. Proceedings of the National Academy of Science, USA 74: 3494–3497.
Ladizinsky, G., 1979. The origin of lentil and its wild genepool. Euphytica 28: 179–187.
Ladizinsky, G., D. Braun, D. Goshen & F.J. Muehlbauer, 1984. The biological species of the genus Lens L. [Lens nigricans]. Botanical gazette 145: 253–261.
Ladizinsky, G., D. Cohen & F.J. Muehlbauer, 1985. Hybridization in the genus Lens by means of embryo culture. Theor Appl Genet 70: 97–101.
Lazaridou, T.B., D.G. Roupakias & A.S. Economou, 1993. Embryo rescue in Vicia faba and Vicia narbonensis. Plant Cell Tiss Org Cult 33: 297–301.
Malik, K.A. & P.K. Saxena, 1992. Thidiazuron induces high frequency shoot regeneration in intact seedlings of pea (Pisum sativum), chickpea (Cicier arietinum) and lentil (Lens culinaris). Aust J Plant Physiol 19: 731–740.
Mejia-Jimenez, A., C. Muñoz, H.J. Jacobsen, W.M. Roca & S.P. Singh, 1994. Interspecific hybridization between common and tepary beans: Increased hybrid embryo growth, fertility, and efficiency through recurrent and congruity backcrossing. Theor Appl Genet 88: 324–331.
McCoy, T.J. & L.Y. Smith, 1986. Interspecific hybridization of perennial Medicago species using ovule-embryo culture. Theor Appl Genet 71: 772–783.
Muehlbauer, F.J., N.F. Weeden & D.L. Hoffman, 1989. Inhertitance and linkage relationships of morphological and isozyme loci in lentil (Lens Miller). J Hered 80: 298–303.
Muehlbauer, F.J., N.F. Weeden & D.L. Hoffman, 1989. Inhertitance and linkage relationships of morphological and isozyme loci in lentil (Lens Miller). J Hered 80: 298–303.
Muench, D.G., A.E. Slinkard & G.J. Scoles, 1991. Determination of genetic variation and taxonomy in lentil (Lens Miller) species by chloroplast DNA polymorphism. Euphytica 56: 213–218.
Murashige, T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497.
Ocampo, B., C. Conicella & J.P. Moss, 2000. Wide crossing: Opportunities and progress. In: R. Knight (Ed.), Linking Research and Marketing Opportunities for Pulses in the 21st Century, Current Plant Science and Biotecnology in Agriculture, Vol. 34, pp. 411-419. Kluwer Academic Publishers, Dordrecht.
Pickersgill, B., 1993. Interspecific hybridization by sexuals means. In: M.D. Hayward, N.O.Bosemark, I. Romagosa (Eds), Plant Breeding: Principles and prospects, pp. 63–78. Chapman & Hill, London.
Pitarelli, G.W. & J.R. Stavely, 1975. Direct hybridisation of Nicotiana rependa , N. tabacum. J Hered 66: 281–284.
Polanco, M.C. & M.L. Ruiz, 1997. Effect of benzylaminopurine on in vitro and in vivo root development in lentil (Lens culinaris Medik.). Plant Cell Rep 17: 22–26.
Polanco, M.C. & M.L. Ruiz, 2001. Factors that affect plant regeneration from in vitro culture of immature seeds in four lentil (Lens culinaris Medik.) cultivars. Plant Cell Tiss Org Cult 66: 133–139.
Saghai-Maroof, M.A., K.M. Soliman, R.A. Jorgensen & R.W. Allard, 1984. Ribosomal DNA spacer length polymorphism in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81: 8014–8018.
Vaillancourt, R.E. & A.E. Slinkard, 1992. Inhertitance of new genetic markers in lentil (Lens Miller). Euphytica 64: 227–236.
Vandenberg, A. & A.E. Slinkard, 1989. Inhertitance of four new quantitative genes in lentil. J Hered 80: 320–322.
Williams, E.G., 1987. Interspecific hybridization in pasture legumes. In: J. Janick (Ed.), Plant Breeding Reviews No 5, pp. 237–305. AVI Publishing Co., Westport.
Williams, E.G., G. Maheswaran, J.F. Hutchinson, 1987. Embryo and ovule culture in crop improvement. In: Janick J. (ed.), Plant Breeding Reviews No 5, pp. 181–236. AVI Publishing Co, Westport.
Wilson, V.E., 1972. Morphology and technique for crossing Lens esculenta Moench. Crop Sci 12: 231–232.
Wilson, V.E. & A.G. Law, 1972. Natural crossing in Lens esculenta Moench. J Am Soc Hortic Sci 97: 142–143.
Ye, G., D.L. McNeil, A.J. Conner & G.D. Hill, 2000. Improved protocol for the multiplication of lentil hybrids without genetic change by culturing single node explants. SABRAO Journal of Breeding and Genetics 32: 13–21.
Ye, G., D.L. McNeil, A.J. Conner & G.D. Hill, 2002. Multiple shoot formation in lentil (Lens culinaris) seeds. New Zealand Journal of Crop and Horticultural Science 30: 1–8.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Fratini, R., Ruiz, M.L. Interspecific Hybridization in the Genus Lens Applying in Vitro Embryo Rescue. Euphytica 150, 271–280 (2006). https://doi.org/10.1007/s10681-006-9118-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10681-006-9118-3